Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.
How do antibodies become validated for Simple Western?
- R&D Systems, Novus Biologics, and valued partners such as Cell Signaling Technology, Bethyl Laboratories, and OriGene are continually validating their antibodies for use with Simple Western.
- Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
- Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.
You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.
*Antibodies validated for Simple Western by supplier and backed by any applicable supplier guarantees
Simple Western Validated Antibodies
| Protein Target | Antibody Type | Vendor / Cat # | Protein Isoform | Citations & Supporting Data | Host Species | Sample | Separation | Dilution | kDa on Simple Western |
|---|---|---|---|---|---|---|---|---|---|
| 11 beta-HSD2 | Primary |
R&D Systems MAB8630 |
Unmodified | Datasheet | Mouse | Primary Human term placenta | Size | 1:100, 1:200, 1:400 | 46 |
| 14-3-3 beta | Primary |
Invitrogen Antibodies PA1-37002 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:1000 | |
| 14-3-3 beta | Primary |
Santa Cruz Biotechnology sc-1657 |
Pan | Datasheet | Mouse | Mouse brain lysates | Size | 1:10, 1:50, 1:100 | 33 |
| 14-3-3 beta | Primary |
Cell Signaling Technology 9636 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 epsilon | Primary |
Cell Signaling Technology 8312 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 epsilon | Primary |
Cell Signaling Technology 9635 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 eta | Primary |
Cell Signaling Technology 9640 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 gamma | Primary |
Novus Biologicals NB100-406 |
Unmodified | Datasheet | Mouse | HeLa lysate 1.0 mg/mL | Size | 1:12.5 | 36 |
| 14-3-3 gamma | Primary |
Novus Biologicals NBP2-81016 |
Datasheet | Mouse | 1:12.5 | ||||
| 14-3-3 gamma | Primary |
Cell Signaling Technology 9637 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 gamma Discontinued |
Primary |
Santa Cruz Biotechnology sc-731 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Size | 1:100 | 39 | |
| 14-3-3 gamma* | Primary |
OriGene TA301660 |
Datasheet | Mouse | HeLa | Size | 1:12.5 | 35 | |
| 14-3-3 tau/theta | Primary |
Novus Biologicals NBP2-27370 |
Unmodified | Datasheet | Rabbit | Human Brain lysate 0.5 mg/mL | Size | 1:10 | 34 |
| 14-3-3 tau/theta | Primary |
Novus Biologicals NBP2-27202 |
Unmodified | Datasheet | Rabbit | Human Brain lysate 0.5 mg/mL | Size | 1:50 | 33 |
| 14-3-3 tau/theta | Primary |
Cell Signaling Technology 9638 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 zeta | Primary |
Cell Signaling Technology 9639 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Brain | Size | 1:50 | |
| 14-3-3 zeta* | Primary |
R&D Systems MAB2669 |
Unmodified | Datasheet | Mouse | SH-SY5Y human neuroblastoma cell line | Size | 2 µg/mL | 34 |
| 15-PGDH/HPGD | Primary |
Novus Biologicals NB200-179 |
Unmodified | Datasheet | Rabbit | Lovo lysate 0.5 mg/mL | Size | 1:500 | 34 |
| 15-PGDH/HPGD* | Primary |
R&D Systems AF5660 |
Unmodified | Datasheet | Goat | Human liver tissue | Size | 10 µg/mL | 34 |
| 15-PGDH/HPGD* | Primary |
Cell Signaling Technology 94019 |
Datasheet | Rabbit | RT-4 | Size | 1:50–1:250 | 33 | |
| 17 beta-HSD1/HSD17B1 | Primary |
Abcam ab51045 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Adipose | Size | 1:10 | 60 |
| 17 beta-HSD1/HSD17B1* | Primary |
R&D Systems MAB7178 |
Unmodified | Datasheet | Mouse | Human placenta tissue | Size | 2.5 µg/mL | 40 |
| 17 beta-HSD1/HSD17B1* | Primary |
R&D Systems AF7178 |
Datasheet | Sheep | Human placenta | Size | 2.5 µg/mL | 40 | |
| 3-Nitro-L-tyrosine | Primary |
Cell Signaling Technology 9691 |
Nitrate |
Citations
(1) Datasheet |
Rabbit | Size | 1:10 | ||
| 4-1BB/TNFRSF9/CD137* | Primary |
R&D Systems MAB8381 |
Unmodified | Datasheet | Mouse | HDLM2 human Hodgkin’s lymphoma cells | Size | 1:20 | 49, 57 |
| 4-1BB/TNFRSF9/CD137* | Primary |
R&D Systems AF838 |
Unmodified | Datasheet | Goat | HDLM2 human Hodgkin’s lymphoma cells | Size | 1:20 | 58 |
| 4-Hydroxynonenal | Primary |
Abcam ab46545 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Redox EVs | 1:25 | ||
| 4-Hydroxynonenal* | Primary |
R&D Systems MAB3249 |
Citations
(1) Datasheet |
Mouse | HepG2 human hepatocellular carcinoma cell line untreated (-) or treated ( + ) with 4-Hydroxynonenal (4-HNE) | Size | 5 µg/mL | ||
| 4E-BP1 | Primary |
Cell Signaling Technology 9455 |
Phospho, Thr70 |
Citations
(1) Datasheet |
Rabbit | Liver | Charge | ||
| 4E-BP1 | Primary |
Cell Signaling Technology 9452 |
Unmodified |
Citations
(15) Datasheet |
Rabbit | Muscle | Size | 1:50 | |
| 4E-BP1 | Primary |
MilliporeSigma 07-1416 |
Unmodified | Rabbit | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562 ± Gleevec | Charge | 1:50–1:200 | ||
| 4E-BP1 | Primary |
Cell Signaling Technology 9456 |
Phospho, Ser65 | Rabbit | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562 ± Gleevec | Charge | 1:50–1:200 | ||
| 4E-BP1 | Primary |
Cell Signaling Technology 9644 |
Unmodified |
Citations
(7) Datasheet |
Rabbit | HepG2 | Size | 1:50 – 1:250 | 24 |
| 4E-BP1 | Primary |
Cell Signaling Technology 2855 |
Phospho, Thr37, 46 |
Citations
(8) Datasheet |
Rabbit | 3T3 | Size | 1:10–1:50 | 23–30 |
| 4E-BP1 | Primary |
Cell Signaling Technology 4923 |
Non-phosphate, Thr46, Thr32, Thr46 | Rabbit | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562 ± Gleevec | Charge | 1:50–1:200 | ||
| 4E-BP1 | Primary |
Cell Signaling Technology 9459 |
Phospho, Thr37, Thr46 |
Citations
(14) Datasheet |
Rabbit | Muscle, Placenta, Mammary glands, Lungs, HDQ-P1 | Size | 1:50, 1:10, 1:200 | |
| 4E-BP1 | Primary |
Epitomics 1557-1 |
Unmodified | Rabbit | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562 ± Gleevec | Charge | 1:50–1:200 | ||
| 4E-BP1 | Primary |
Cell Signaling Technology 9451 |
Phospho, Ser65 |
Citations
(4) Datasheet |
Rabbit | K562 ± Gleevec lysed in Bicine CHAPS, EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562 ± Gleevec | Size | 1:50 | 27-31 |
| 4E-BP1 | Primary |
Epitomics 2334-1 |
Phospho | Rabbit | EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562 ± Gleevec | Charge | 1:50–1:200 | ||
| 4E-BP1* | Primary |
R&D Systems AF3227 |
Datasheet | Goat | HeLa human cervical epithelial carcinoma cell line | Size | 5 µg/mL | 26 | |
| 4E-BP1* | Primary |
Cell Signaling Technology 13443 |
Phospho, Ser65 | Datasheet | Rabbit | a172 + Mg132 (10 µM, O/N) | Size | 1:10–1:50 | 12 |
| 4E-BP2 | Primary |
Cell Signaling Technology 2845 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | SH-SY5Y | Size, Charge | ||
| 4HNE | Primary |
Cell Biolabs STA-035 |
Citations
(1) Datasheet |
Rabbit | Size | ||||
| 5'-Nucleotidase/CD73 | Primary |
ABclonal A2029 |
Unmodified |
Citations
(1) Datasheet |
Rabbit | Bladder | Size | ||
| 5'-Nucleotidase/CD73* | Primary |
R&D Systems AF5795 |
Citations
(1) Datasheet |
Sheep | U266 human myeloma cell line | Size | 10 µg/mL | 86 | |
| 5'-Nucleotidase/CD73* | Primary |
R&D Systems MAB57951 |
Datasheet | Mouse | U251MG human glioblastoma cell line and A431 human epithelial carcinoma cell line | Size | 20 µg/mL | 86 | |
| 5-HT1A | Primary |
Novus Biologicals NBP2-21590 |
Unmodified |
Citations
(5) Datasheet |
Rabbit | ||||
| 5-HT1D | Primary |
Novus Biologicals NB100-56349 |
Datasheet | Rabbit | Human Brain lysate 0.5 mg/mL | Size | 1:10 | ||
| 5-HT2A | Primary |
LSBio LS-C172270 |
Unmodified |
Citations
(2) Datasheet |
Rabbit | Rat cortex | Size | 1:500 | |
| 5-HT2C | Primary |
Santa Cruz Biotechnology sc-17797 |
Unmodified |
Citations
(2) Datasheet |
Mouse | Rat brain fractions | Size | 1:50 | 55 |