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Simple Western Antibody Database

Find Antibodies Validated for Simple Western

Simple Western™ systems are open platforms, meaning the possibilities are almost endless when selecting antibodies to develop your assays. The Simple Western Antibody database is curated to facilitate the selection process by providing general assay development guidance for identifying and selecting antibodies to test. These guidelines are provided to save time with recommended starting points to optimize for the biological system being examined.

How do antibodies become validated for Simple Western?

  • R&D Systems, Novus Biologics, and valued partners such as Cell Signaling Technology, Bethyl Laboratories, and OriGene are continually validating their antibodies for use with Simple Western.
  • Help other scientists and fund your research! Submit new antibody validation data to the Simple Western antibody database and receive a free 8x25 separation module or detection module. Learn more about the Simple Western Antibody Validation Promotion.
  • Our expert in-house scientists continue to validate antibodies and review customer-submitted data to ensure the recommendations meet our high standards, so come back for the latest list of validated antibodies in the future.

You can sort by column headings or just type into the Search box. If you don't see your antibody listed here, email us at support@proteinsimple.com.

*Antibodies validated for Simple Western by supplier and backed by any applicable supplier guarantees

Simple Western Validated Antibodies


Filter Antibodies: (7852)
Protein Target Antibody Type Vendor / Cat # Protein Isoform Citations & Supporting Data Host Species Sample Separation Dilution kDa on Simple Western
11 beta-HSD2 Primary R&D Systems
MAB8630
Unmodified Datasheet Mouse Primary Human term placenta Size 1:100, 1:200, 1:400 46
14-3-3 beta Primary Invitrogen Antibodies
PA1-37002
Unmodified Citations (1)
Datasheet
Rabbit Brain Size 1:1000
14-3-3 beta Primary Santa Cruz Biotechnology
sc-1657
Pan Datasheet Mouse Mouse brain lysates Size 1:10, 1:50, 1:100 33
14-3-3 beta Primary Cell Signaling Technology
9636
Unmodified Citations (1)
Datasheet
Rabbit Brain Size 1:50
14-3-3 epsilon Primary Cell Signaling Technology
8312
Unmodified Citations (1)
Datasheet
Rabbit Brain Size 1:50
14-3-3 epsilon Primary Cell Signaling Technology
9635
Unmodified Citations (1)
Datasheet
Rabbit Brain Size 1:50
14-3-3 eta Primary Cell Signaling Technology
9640
Unmodified Citations (1)
Datasheet
Rabbit Brain Size 1:50
14-3-3 gamma Primary Novus Biologicals
NB100-406
Unmodified Datasheet Mouse HeLa lysate 1.0 mg/mL Size 1:12.5 36
14-3-3 gamma Primary Novus Biologicals
NBP2-81016
Datasheet Mouse 1:12.5
14-3-3 gamma Primary Cell Signaling Technology
9637
Unmodified Citations (1)
Datasheet
Rabbit Brain Size 1:50
14-3-3 gamma
Discontinued
Primary Santa Cruz Biotechnology
sc-731
Unmodified Citations (1)
Datasheet
Rabbit Size 1:100 39
14-3-3 gamma* Primary OriGene
TA301660
Datasheet Mouse HeLa Size 1:12.5 35
14-3-3 tau/theta Primary Novus Biologicals
NBP2-27370
Unmodified Datasheet Rabbit Human Brain lysate 0.5 mg/mL Size 1:10 34
14-3-3 tau/theta Primary Novus Biologicals
NBP2-27202
Unmodified Datasheet Rabbit Human Brain lysate 0.5 mg/mL Size 1:50 33
14-3-3 tau/theta Primary Cell Signaling Technology
9638
Unmodified Citations (1)
Datasheet
Rabbit Brain Size 1:50
14-3-3 zeta Primary Cell Signaling Technology
9639
Unmodified Citations (1)
Datasheet
Rabbit Brain Size 1:50
14-3-3 zeta* Primary R&D Systems
MAB2669
Unmodified Datasheet Mouse SH-SY5Y human neuroblastoma cell line Size 2 µg/mL 34
15-PGDH/HPGD Primary Novus Biologicals
NB200-179
Unmodified Datasheet Rabbit Lovo lysate 0.5 mg/mL Size 1:500 34
15-PGDH/HPGD* Primary R&D Systems
AF5660
Unmodified Datasheet Goat Human liver tissue Size 10 µg/mL 34
15-PGDH/HPGD* Primary Cell Signaling Technology
94019
Datasheet Rabbit RT-4 Size 1:50–1:250 33
17 beta-HSD1/HSD17B1 Primary Abcam
ab51045
Unmodified Citations (1)
Datasheet
Rabbit Adipose Size 1:10 60
17 beta-HSD1/HSD17B1* Primary R&D Systems
MAB7178
Unmodified Datasheet Mouse Human placenta tissue Size 2.5 µg/mL 40
17 beta-HSD1/HSD17B1* Primary R&D Systems
AF7178
Datasheet Sheep Human placenta Size 2.5 µg/mL 40
3-Nitro-L-tyrosine Primary Cell Signaling Technology
9691
Nitrate Citations (1)
Datasheet
Rabbit Size 1:10
4-1BB/TNFRSF9/CD137* Primary R&D Systems
MAB8381
Unmodified Datasheet Mouse HDLM2 human Hodgkin’s lymphoma cells Size 1:20 49, 57
4-1BB/TNFRSF9/CD137* Primary R&D Systems
AF838
Unmodified Datasheet Goat HDLM2 human Hodgkin’s lymphoma cells Size 1:20 58
4-Hydroxynonenal Primary Abcam
ab46545
Unmodified Citations (1)
Datasheet
Rabbit Redox EVs 1:25
4-Hydroxynonenal* Primary R&D Systems
MAB3249
Citations (1)
Datasheet
Mouse HepG2 human hepatocellular carcinoma cell line untreated (-) or treated ( + ) with 4-Hydroxynonenal (4-HNE) Size 5 µg/mL
4E-BP1 Primary Cell Signaling Technology
9455
Phospho, Thr70 Citations (1)
Datasheet
Rabbit Liver Charge
4E-BP1 Primary Cell Signaling Technology
9452
Unmodified Citations (15)
Datasheet
Rabbit Muscle Size 1:50
4E-BP1 Primary MilliporeSigma
07-1416
Unmodified Rabbit EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562 ± Gleevec Charge 1:50–1:200
4E-BP1 Primary Cell Signaling Technology
9456
Phospho, Ser65 Rabbit EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562 ± Gleevec Charge 1:50–1:200
4E-BP1 Primary Cell Signaling Technology
9644
Unmodified Citations (7)
Datasheet
Rabbit HepG2 Size 1:50 – 1:250 24
4E-BP1 Primary Cell Signaling Technology
2855
Phospho, Thr37, 46 Citations (8)
Datasheet
Rabbit 3T3 Size 1:10–1:50 23–30
4E-BP1 Primary Cell Signaling Technology
4923
Non-phosphate, Thr46, Thr32, Thr46 Rabbit EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562 ± Gleevec Charge 1:50–1:200
4E-BP1 Primary Cell Signaling Technology
9459
Phospho, Thr37, Thr46 Citations (14)
Datasheet
Rabbit Muscle, Placenta, Mammary glands, Lungs, HDQ-P1 Size 1:50, 1:10, 1:200
4E-BP1 Primary Epitomics
1557-1
Unmodified Rabbit EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562 ± Gleevec Charge 1:50–1:200
4E-BP1 Primary Cell Signaling Technology
9451
Phospho, Ser65 Citations (4)
Datasheet
Rabbit K562 ± Gleevec lysed in Bicine CHAPS, EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562 ± Gleevec Size 1:50 27-31
4E-BP1 Primary Epitomics
2334-1
Phospho Rabbit EGF stimulated MCF10A, Ly294002 inhibited MCF7, K562 ± Gleevec Charge 1:50–1:200
4E-BP1* Primary R&D Systems
AF3227
Datasheet Goat HeLa human cervical epithelial carcinoma cell line Size 5 µg/mL 26
4E-BP1* Primary Cell Signaling Technology
13443
Phospho, Ser65 Datasheet Rabbit a172 + Mg132 (10 µM, O/N) Size 1:10–1:50 12
4E-BP2 Primary Cell Signaling Technology
2845
Unmodified Citations (1)
Datasheet
Rabbit SH-SY5Y Size, Charge
4HNE Primary Cell Biolabs
STA-035
Citations (1)
Datasheet
Rabbit Size
5'-Nucleotidase/CD73 Primary ABclonal
A2029
Unmodified Citations (1)
Datasheet
Rabbit Bladder Size
5'-Nucleotidase/CD73* Primary R&D Systems
AF5795
Citations (1)
Datasheet
Sheep U266 human myeloma cell line Size 10 µg/mL 86
5'-Nucleotidase/CD73* Primary R&D Systems
MAB57951
Datasheet Mouse U251MG human glioblastoma cell line and A431 human epithelial carcinoma cell line Size 20 µg/mL 86
5-HT1A Primary Novus Biologicals
NBP2-21590
Unmodified Citations (5)
Datasheet
Rabbit
5-HT1D Primary Novus Biologicals
NB100-56349
Datasheet Rabbit Human Brain lysate 0.5 mg/mL Size 1:10
5-HT2A Primary LSBio
LS-C172270
Unmodified Citations (2)
Datasheet
Rabbit Rat cortex Size 1:500
5-HT2C Primary Santa Cruz Biotechnology
sc-17797
Unmodified Citations (2)
Datasheet
Mouse Rat brain fractions Size 1:50 55

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