What receptors does FGF basic bind?

FGF receptor specificity has been reviewed in multiple citations. Please find more information at: //www.rndsystems.com/resources/articles/fibroblast-growth-factors-and-their-receptors

Does human FGF basic show activity on mouse cells?

Yes, it does. The bioassay uses NR-6 mouse fibroblast cells. There is 95% homology between the human and mouse protein and 98% homology between the human and mouse receptor.

Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade, CF

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 4114-TC

Citations (21)

(1)

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Source:	E. coli
Accession #:	NP_001997
Applications:	Bioactivity

View Full Technical Details for Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade, CF

Product Specifications for Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade, CF

Source	E. coli-derived human FGF basic/FGF2/bFGF protein Ala144-Ser288
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala144
Predicted Molecular Mass	16 kDa
Activity	This protein was used at 20-50 ng/mL to support a 100-fold expansion of rat cortical neural stem cells or mouse cortical stem cells. Cells were cultured in N2 plus medium and supplemented with fresh FGF basic/FGF2/bFGF every day. Medium was changed every other day and cells were split every 2-4 days upon becoming 70% confluent. Measured in a cell proliferation assay using NR6R‑3T3 mouse fibroblast cells. Raines, E.W. et al. (1985) Methods Enzymol. 109:749. The ED₅₀ for this effect is 0.3-3 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4114-TC

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris-HCl and NaCl.
Reconstitution	Reconstitute at 0.5-1.0 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF basic/FGF2/bFGF

FGF basic (also known as FGF2 and HBGF-2) is an 18-34 kDa, heparin-binding member of the FGF superfamily of molecules (1-3). Superfamily members are characterized by the presence of a centrally placed beta -trefoil structure. FGF acidic (FGF1) and FGF basic (FGF2) were the first two identified FGFs, and the designations acidic and basic refer to their relative isoelectric points. Human FGF basic is 288 amino acids (aa) in length. There are multiple start sites, four of which utilize atypical CUG codons, and one that initiates at an AUG start site (4-6). The four CUG start sites generate high molecular weight (HMW) FGF basic. There is a 34 kDa, 288 aa form, a 24 kDa, 210 aa form, a 22.5 kDa, 201 aa form, and a 22 kDa, 196 aa form. All are retained intracellularly, undergo extensive methylation, and possess one or more nuclear localization signals (NLS) (7-9). The AUG initiating form is 18 kDa and 155 aa in length. There is no signal sequence (ss). It is, however, secreted directly through the plasma membrane via a mechanism that appears to be dependent upon tertiary structure (10). In place of a ss, there is purportedly a 9 aa N-terminal prosegment that precedes a 146 aa mature segment (11). Early isolations of 18 kDa bovine FGF basic yielded 146 aa molecules, an effect attributed to the presence of acid proteases (12). The molecule contains a heparin-binding site (aa residues 128-144), and undergoes phosphorylation at Ser117 (13). There is also an ill-defined C-terminal NLS that may be more “functional” (or 3-dimensional) than structural (7). Human 146 aa FGF basic is 97% aa identical to mouse FGF basic (14).

Item	Value
References	Sorenson, V. et al. (2006) BioEssays 28:504. Kardami, E. et al. (2004) Cardiovasc. Res. 63:458. Nugent, M.A. and R.V. Lozzo (2000) Int. J. Biochem. Cell Biol. 32:115. Abraham, J.A. et al. (1986) EMBO J. 5:2523. Prats, H. et al. (1989) Proc. Natl. Acad. Sci. USA 86:1836. Arnaud, E. et al. (1999) Mol. Cell. Biol. 19:505. Foletti, A. et al. (2003) Cell. Mol. Life Sci. 60:2254. Arese, M. et al. (1999) Mol. Biol. Cell 10:1429. Pintucci, G. et al. (1996) Mol. Biol. Cell 7:1249. Nickel, W. (2005) Traffic 6:607. SwissProt # P09038. Klagsbrun, M. et al. (1987) Proc. Natl. Acad. Sci. USA 84:1839. Bailly, K. et al. (2000) FASEB J. 14:333. Hebert, J.M. et al. (1990) Dev. Biol. 138:454.
Long Name	Fibroblast Growth Factor basic
Entrez Gene IDs	2247 (Human); 14173 (Mouse); 281161 (Bovine); 403857 (Canine); 100033955 (Equine)
Alternate Names	Basic fibroblast growth factor, FGF basic, FGF-2, FGF2, FGFBprostatropin, HBGF-2, Prostatropin, bFGF, basic fibroblast growth factor bFGF, fibroblast growth factor 2 (basic), heparin-binding growth factor 2

Citations for Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade, CF (21)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Kobayashi et al. (2022), Expanding homogeneous culture of human primordial germ cell-like cells maintaining germline features without serum or feeder layers Stem Cell Reports, 0(0).
PMID: 35148847
Species: Human
Sample Types: Cell Culture Supernates
Applications: Bioassay

L Mahoney-Sa et al. (2022), Alpha synuclein determines ferroptosis sensitivity in dopaminergic neurons via modulation of ether-phospholipid membrane composition Cell Reports, 40(8):111231.
PMID: 36001957
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

D Sardar et al. (2022), Sox9 directs divergent epigenomic states in brain tumor subtypes Oncogene, 119(29):e2202015119.
PMID: 35858326
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

EJ Kim et al. (2022), The oncogenic JAG1 intracellular domain is a transcriptional cofactor that acts in concert with DDX17/SMAD3/TGIF2 Cell Reports, 41(8):111626.
PMID: 36417870
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

AN Cho et al. (2021), Microfluidic device with brain extracellular matrix promotes structural and functional maturation of human brain organoids Nature Communications, 12(1):4730.
PMID: 34354063
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SG Kim et al. (2021), Tanc2-mediated mTOR inhibition balances mTORC1/2 signaling in the developing mouse brain and human neurons Nature Communications, 12(1):2695.
PMID: 33976205
Species: Human
Sample Types: Spheroid
Applications: Bioassay

L Jerby-Arno et al. (2021), Opposing immune and genetic mechanisms shape oncogenic programs in synovial sarcoma Nature Medicine, 0(0).
PMID: 33495604
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Bae et al. (2021), SLC6A20 transporter: a novel regulator of brain glycine homeostasis and NMDAR function Embo Molecular Medicine, 0(0):e12632.
PMID: 33428810
Species: Mouse

Applications: Cell Culture

N Micali et al. (2020), Variation of Human Neural Stem Cells Generating Organizer States Inï¿½Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates Cell Rep, 31(5):107599.
PMID: 32375049
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

EAA Obara et al. (2020), SPT6-driven error-free DNA repair safeguards genomic stability of glioblastoma cancer stem-like cells Nat Commun, 11(1):4709.
PMID: 32948765
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Zhang et al. (2020), Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State Cell Stem Cell, 0(0).
PMID: 32783886
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

W Tao et al. (2020), SATB2 drives glioblastoma growth by recruiting CBP to promote FOXM1 expression in glioma stem cells EMBO Mol Med, 0(0):e12291.
PMID: 33124191
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Zhang et al. (2020), Generation of Functional Brown Adipocytes from Human Pluripotent Stem Cells via Progression through a Paraxial Mesoderm State Cell Stem Cell, 0(0).
PMID: 32783886
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

T Colunga et al. (2019), Human Pluripotent Stem Cell-Derived Multipotent Vascular Progenitors of the Mesothelium Lineage Have Utility in Tissue Engineering and Repair Cell Rep, 26(10):2566-2579.e10.
PMID: 30840882
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

JA Bernatchez et al. (2019), Activity of Selected Nucleoside Analogue ProTides against Zika Virus in Human Neural Stem Cells Viruses, 11(4).
PMID: 31010044
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

J Cholewa-Wa et al. (2019), Quantitative modelling predicts the impact of DNA methylation on RNA polymerase II traffic Proc. Natl. Acad. Sci. U.S.A., 116(30):14995-15000.
PMID: 31289233
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

S Beck et al. (2019), Mechanism of Action of Methotrexate Against Zika Virus Viruses, 11(4).
PMID: 30974762
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

EK Kim et al. (2017), Ursodeoxycholic acid inhibits the proliferation of colon cancer cells by regulating oxidative stress and cancer stem-like cell growth PLoS ONE, 12(7):e0181183.
PMID: 28708871
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kristen M Bullard-Fe (2016), The FDA-approved drug sofosbuvir inhibits Zika virus infection Antiviral Res, 137(0):134-140.
PMID: 27902933
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Adepoju A et al. (2014), FGF2 and insulin signaling converge to regulate cyclin D expression in multipotent neural stem cells. Stem Cells, 32(3):770-8.
PMID: 24155149
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

West FD et al. (2008), Enrichment and differentiation of human germ-like cells mediated by feeder cells and basic fibroblast growth factor signaling. Stem Cells, 26(11):2768-76.
PMID: 18719225

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Customer Reviews for Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade, CF (1)

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Recombinant Human FGF basic, 145 aa (TC Grade) Protein, CF

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FAQs for Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade, CF

What receptors does FGF basic bind?

FGF receptor specificity has been reviewed in multiple citations. Please find more information at: //www.bio-techne.com/resources/articles/fibroblast-growth-factors-and-their-receptors
Does human FGF basic show activity on mouse cells?

Yes, it does. The bioassay uses NR-6 mouse fibroblast cells. There is 95% homology between the human and mouse protein and 98% homology between the human and mouse receptor.

Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: FGF basic/FGF2/bFGF

Citations for Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade, CF (21)

Customer Reviews for Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade, CF (1)

Recombinant Human FGF basic, 145 aa (TC Grade) Protein, CF

FAQs for Recombinant Human FGF basic/FGF2/bFGF, 145 aa TC Grade, CF

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