Human Oct-4A Antibody

Name: Human Oct-4A Antibody
Brand: R&D Systems
SKU: MAB17591
Rating: 4 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB17591

Citations (3)

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Species Reactivity:	Human
Immunogen:	E.coli-derived recombinant human Oct-4A Met1-Glu135 Accession # NP_002692
Applications:	CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG_2A Clone # 653108
Specificity:	Detects human Oct-4A in Western blots.

View Full Technical Details for Human Oct-4A Antibody

Applications for Human Oct-4A Antibody

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below
Intracellular Staining by Flow Cytometry	2.5 µg/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Oct-4A Antibody

	Western Blot Detection of Human Oct‑4A by Western Blot. Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and NTera-2 human testicular embryonic carcinoma cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human Oct-4A Monoclonal Antibody (Catalog # MAB17591) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Oct-4A at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunocytochemistry Oct-4A and E-Cadherin in BG01V Human Stem Cells. Oct-4A and E-Cadherin were detected in human BG01V embryonic stem cells grown on irradiated MEF cells using 10 µg/mL Human Oct-4A Monoclonal Antibody (Catalog # MAB17591) and 10 µg/mL Human E-Cadherin Affinity-purified Polyclonal Antibody (Catalog # AF648). Cells were incubated with primary antibodies for 3 hours at room temperature. Cells were stained for Oct-4A using the Northern-Lights™ 493-conjugated Anti-Mouse IgG Secondary Antibody (green; Catalog # NL009), and stained for E-Cadherin using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001). Specific staining of Oct-4A was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Intracellular Staining by Flow Cytometry Detection of Oct‑4A in BG01V Human Stem Cells by Flow Cytometry. BG01V human embryonic stem cells was stained with Human Oct-4A Monoclonal Antibody (Catalog # MAB17591, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Reconstitution	Sterile PBS to a final concentration of 0.5 mg/mL. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Oct-4A

Oct-3/4, alternately Oct-3 or Oct-4, is POU5F1 (POU domain containing, class 5, transcription factor 1), a 360 amino acid (aa) transcription factor that is expressed in totipotent embryonic stem and germ cells. The human Oct-4, Oct-3/4 or POU5F1 gene can be transcribed into at least three transcripts (Oct-4A, Oct-4B, and Oct-4B1) and generates four protein isoforms by alternative splicing and alternative translation initiation. Oct-4A expression is restricted to embryonic stem (ES) and embryonic carcinoma (EC) cells and is believed to be the transcription factor responsible for the pluripotency properties of embryonic stem (ES) cells. In contrast, Oct-4B/4B1 can be detected in various nonpluripotent cell types and cannot sustain ES cell pluripotency and self-renewal.

Item	Value
Long Name	Octamer-binding Transcription Factor 4A
Entrez Gene IDs	5460 (Human); 18999 (Mouse)
Alternate Names	OCT3;Oct-3;OCT4;Oct-4;OCT4;Oct3/4;OTF3;OTF-3;OTF4;POU domain protein;POU domain, class 5, transcription factor 1;POU5F1, Oct-4A, Oct4A

Citations for Human Oct-4A Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Human Oct-4A Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: ICC

YC Lin et al. (2021-01-01), Interactions between ALS-linked FUS and nucleoporins are associated with defects in the nucleocytoplasmic transport pathway Nature Neuroscience, 0(0).
PMID: 34059832
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

F Vlahova et al. (2019-01-01), Human mid-trimester amniotic fluid (stem) cells lack expression of the pluripotency marker OCT4A Sci Rep, 9(1):8126.
PMID: 31148575
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

(2016-01-01), Genome-Wide Transcriptome and Binding Sites Analyses Identify Early FOX Expressions for Enhancing Cardiomyogenesis Efficiency of hESC Cultures Sci Rep, 6(0):31068.
PMID: 27501774

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Customer Reviews for Human Oct-4A Antibody (2)

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Human Oct-4A Antibody

Name: Anonymous

Application: WB

Sample Tested: NTera-2 human testicular embryonic carcinoma cell line

Species: Human

Verified Customer | Posted 03/12/2022
Human Oct-4A Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: WS-1 human fetal skin fibroblast cell line

Species: Human

Verified Customer | Posted 01/31/2019

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Product Documents for Human Oct-4A Antibody

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Human Oct-4A Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human Oct-4A Antibody

Scientific Data Examples for Human Oct-4A Antibody

Western Blot

Immunocytochemistry

Intracellular Staining by Flow Cytometry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Oct-4A

Citations for Human Oct-4A Antibody (3)

Customer Reviews for Human Oct-4A Antibody (2)

Customer Images

Human Oct-4A Antibody

Human Oct-4A Antibody

Product Documents for Human Oct-4A Antibody

Product Datasheets

COA

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SDS

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