Human MYH7 Antibody

Name: Human MYH7 Antibody
Brand: R&D Systems
SKU: MAB90961
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB90961

Citations (1)

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Recombinant Monoclonal Antibody.

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Key Product Details

Species Reactivity:	Human
Immunogen:	Human MYH7 synthetic peptide Accession # P12883
Applications:	Immunocytochemistry/Immunofluorescence, Western Blot
Label:	Unconjugated
Source:	Recombinant Monoclonal Rabbit IgG Clone # 2021A
Specificity:	Detects human MYH7 in direct ELISAs and Western blots.

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Applications for Human MYH7 Antibody

	Recommended Concentration	Sample
Western Blot	0.5 µg/mL	See below
Immunocytochemistry	5-25 µg/mL	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human MYH7 Antibody

	Western Blot Detection of MYH7 by Western Blot. Western blot shows lysates of human heart (ventricle) tissue. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB90961) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for MYH7 at approximately 230 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunocytochemistry MYH7 in Human Cardiomyocytes. MYH7 was detected in immersion fixed BG01V human embryonic stem cells differentiated into cardiomyocytes using Rabbit Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB90961) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Formulation, Preparation and Storage

Purification	Protein A or G purified from cell culture supernatant
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: MYH7

MYH7 encodes the beta myosin heavy chain (MHC-beta ) which is a component of cardiac muscle myosin mainly expressed in the ventricle of fetal heart and represents the minority myosin in the adult heart. This is the 'slow form' of cardiac myosin as opposed to the 'fast form' (MYH6, aka MHC-alpha ) expressed more predominantely in the atria of the fetal heart and is the predominant myosin in the adult heart. The two isoforms of cardiac MHC alpha and beta display 93% homology but have significantly different enzymatic properties, with alpha having 150-300% the contractile velocity and 60-70% actin attachment time as that of beta. Several mutations in MYH7 have been associated with inherited cardiomyopathies paraspinal and proximal muscle atrophy. MYH7 is a 223 kDa protein composed of 1935 amino acids.

Item	Value
Long Name	Myosin Heavy Chain 7, Cardiac Muscle, Beta
Entrez Gene IDs	4625 (Human); 140781 (Mouse); 29557 (Rat)
Alternate Names	CMD1S, CMD1SMGC138376, CMH1, DKFZp451F047, MGC138378, MPD1, MYH7, MYHCB, MyHC-beta, Myosin-7, SPMD, SPMM, cardiac muscle beta isoform, myhc-slow, myopathy, distal 1, myosin heavy chain (AA 1-96), myosin, heavy chain 7, cardiac muscle, beta, myosin, heavy polypeptide 7, cardiac muscle, beta, rhabdomyosarcoma antigen MU-RMS-40.7A

Product Specific Notices for Human MYH7 Antibody

Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.

Citations for Human MYH7 Antibody (1)

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Citations are publications that use Bio-Techne products. Selected citations for Human MYH7 Antibody include:

Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot

Y Zhao et al. (2018-01-01), An essential role for Wnt/?-catenin signaling in mediating hypertensive heart disease Sci Rep, 8(1):8996.
PMID: 29895976

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Human MYH7 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: human iPSC-derived cardiomyocytes

Species: Human

Verified Customer | Posted 03/05/2019

the antibody was used for immunofluorescence staining on day 15 human iPSC-derived cardiomyocytes (the day of initiation of differentiation from iPSCs is counted as day 0).

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Product Documents for Human MYH7 Antibody

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Human MYH7 Antibody

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Applications for Human MYH7 Antibody

Scientific Data Examples for Human MYH7 Antibody

Western Blot

Immunocytochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: MYH7

Product Specific Notices for Human MYH7 Antibody

Citations for Human MYH7 Antibody (1)

Customer Reviews for Human MYH7 Antibody (1)

Customer Images

Human MYH7 Antibody

Product Documents for Human MYH7 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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