What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.

How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?

All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.

How should Cultrex Basement Membrane Extract (BME) be stored and handled?

Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract

Name: Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract
Brand: R&D Systems
SKU: 3434-010-02

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 3434-010-02

Citations (8)

Product Datasheet / COA / SDS

Basement membrane extract specifically qualified to support feeder-free stem cell culture for embryonic and pluripotent stem cells.

Try it on your cultures! Request a Sample of Cultrex Stem Cell Qualified RGF BME

Key Product Details

Features:	Cultrex Stem Cell Qualified RGF Basement Membrane Extract (BME) has been shown to provide an effective feeder-free surface for the attachment and maintenance of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) in culture.
Key Benefits:	Qualified to support the culture of pluripotent stem cell Supports human iPSC and ESC expansion and differentiation Reduced growth factor formulation provides a more defined culture system Quality controlled for performance consistency

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Citations (8) / Reviews / FAQs

Summary

Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract (RGF BME) has been shown to provide anprovides effective feeder-free surface for the attachment and maintenance of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) in culture.

Why Use Cultrex Stem Cell Qualified RGF BME?

Cultrex Stem Cell Qualified RGF Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Cultrex Stem Cell Qualified RGF BME has been shown to provide an effective surface for feeder-free stem cell attachment and maintenance of undifferentiated human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs). It is useful for promoting the expansion of pluripotent stem cells or for the study of stem cell differentiation.

Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparin sulfate proteoglycans.

Product Specifications

Species	Mouse
Source	Murine Engelbreth-Holm-Swarm (EHS) tumor
Protein Concentration	8-12 mg/mL as determined by Lowry assay
Endotoxin level	≤ 8 EU/mL by Limulus Amoebocyte Lysate (LAL) assay
Sterility Testing	No bacterial or fungal growth detected following 14 days in culture
Cell Culture Testing	Promotes the human induced pluripotent stem cell attachment. Effectively maintains human iPSCs in a pluripotent state in a feeder-free stem cell culture.
Viral Testing	Tested negative by PCR test for a total of 31 organisms and viruses, including: mycoplasma, 17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, and 13 additional murine infectious agents including LDEV.

Scientific Data Examples

	Cultrex BME Improves Hematopoietic Stem Cell Differentiation from Induced Pluripotent Stem Cells (iPSCs). Human iPSCs were grown for a minimum of 2 passages on either Cultrex Stem Cell Qualified RGF BME (Catalog # 3434-005-02) or a leading competitor's matrix, prior to creating embryoid bodies for hematopoietic stem cell differentiation. Cells pre-cultured on Cultrex Stem Cell Qualified RGF BME, showed a greater efficiency to develop into hematopoietic stem cells (CD34⁺, CD45⁺). Data courtesy of the Verneris Laboratory at the University of Colorado.
	Colonies of iPSCs Grown on Cultrex Stem Cell Qualified RGF BME. Human erythroblasts, reprogrammed into induced pluripotent stem cells (iPSCs), were cultured on plates coated with Cultrex Stem Cell Qualified RGF BME (Catalog # 3434-005-02). Example colonies are shown at 4X magnification. Data courtesy of the Harvard Stem Cell Core.

Preparation & Storage

Stability	Product is stable for at least two years from date of manufacture when stored at ≤ -70 °C. See lot specific Certificate of Analysis for expiration date.
Shipping Conditions	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended on the product label.
Storage	Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

Citations for Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract (8)

Showing 1 - 8 of 8 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

KJ Choi et al. (2022), NANOG prion-like assembly mediates DNA bridging to facilitate chromatin reorganization and activation of pluripotency Nature Cell Biology, 0(0).
PMID: 35484250
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

MP Pappas et al. (2022), Defining the Skeletal Myogenic Lineage in Human Pluripotent Stem Cell-Derived Teratomas Cells, 11(9).
PMID: 35563894
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Z Wang et al. (2022), Dominant role of DNA methylation over H3K9me3 for IAP silencing in endoderm Nature Communications, 13(1):5447.
PMID: 36123357
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Seah et al. (2022), Modeling gene ï¿½ environment interactions in PTSD using human neurons reveals diagnosis-specific glucocorticoid-induced gene expression Nature Neuroscience, 25(11):1434-1445.
PMID: 36266471
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Peron et al. (2021), Generation of two human iPSC lines, FINCBi002-A and FINCBi003-A, carrying heteroplasmic macrodeletion of mitochondrial DNA causing Pearson's syndrome Stem Cell Research, 50(0):102151.
PMID: 33434818
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Peron et al. (2020), Generation of a human iPSC line, FINCBi001-A, carrying a homoplasmic m.G3460A mutation in MT-ND1 associated with Leberï¿½s Hereditary optic Neuropathy (LHON) Stem Cell Res, 48(0):101939.
PMID: 32771908
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

C Peron et al. (2020), Generation of a human iPSC line, FINCBi001-A, carrying a homoplasmic m.G3460A mutation in MT-ND1 associated with Leberï¿½s Hereditary optic Neuropathy (LHON) Stem Cell Res, 48(0):101939.
PMID: 32771908
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

GE Vázquez-Vé et al. (2019), Doublecortin like kinase 1 regulates alpha-Synuclein levels and toxicity J. Neurosci., 0(0).
PMID: 31748376

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Showing 1 - 8 of 8 citations Showing All

FAQs

What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?

Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex^® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.
How does Cultrex^® Basement Membrane Extract (BME) promote cell differentiation?

All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.
How should Cultrex Basement Membrane Extract (BME) be stored and handled?

Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.

Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Summary

Why Use Cultrex Stem Cell Qualified RGF BME?

Product Specifications

Scientific Data Examples

Preparation & Storage

Citations for Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract (8)

FAQs

Product Documents

Product Datasheets

COA

Certificate of Analysis

SDS

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