Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract
Key Product Details
Features: | Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract (RGF BME) has been shown to provide an effective feeder-free surface for the attachment and maintenance of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) in culture. |
Key Benefits: |
|
Order Information
Shipping Information
$35 to United States
Products in stock will be shipped in 1-3 business days unless specified.
Assay Procedure
Citations for Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract (2)
Citations are publications that use Bio-Techne products. Selected citations for Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract include:
-
Species: Human
Sample Types: Whole Cells
Applications: BioassayC Peron et al. (2020), Generation of a human iPSC line, FINCBi001-A, carrying a homoplasmic m.G3460A mutation in MT-ND1 associated with Leber�s Hereditary optic Neuropathy (LHON) Stem Cell Res, 48(0):101939.
PMID: 32771908 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayGE Vázquez-Vé et al. (2019), Doublecortin like kinase 1 regulates alpha-Synuclein levels and toxicity J. Neurosci., 0(0).
PMID: 31748376
There are no citations that match your criteria.
FAQs
-
What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?
Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.
-
How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?
All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.
-
How should Cultrex Basement Membrane Extract (BME) be stored and handled?
Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.