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Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract
Catalog # 3434-010-02
Key Product Details
| Features: | Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract (RGF BME) has been shown to provide an effective feeder-free surface for the attachment and maintenance of human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) in culture. |
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Bio-Techne Corporation
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Assay Procedure
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Cultrex BME Improves Hematopoietic Stem Cell Differentiation from Induced Pluripotent Stem Cells (iPSCs). Human iPSCs were grown for a minimum of 2 passages on either Cultrex Stem Cell Qualified RGF BME (Catalog # 3434-005-02) or a leading competitor's matrix, prior to creating embryoid bodies for hematopoietic stem cell differentiation. Cells pre-cultured on Cultrex Stem Cell Qualified RGF BME, showed a greater efficiency to develop into hematopoietic stem cells (CD34+, CD45+). Data courtesy of the Verneris Laboratory at the University of Colorado. |
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Colonies of iPSCs Grown on Cultrex Stem Cell Qualified RGF BME. Human erythroblasts, reprogrammed into induced pluripotent stem cells (iPSCs), were cultured on plates coated with Cultrex Stem Cell Qualified RGF BME (Catalog # 3434-005-02). Example colonies are shown at 4X magnification. Data courtesy of the Harvard Stem Cell Core. |
Citations for Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract (2)
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Citations are publications that use Bio-Techne products. Selected citations for Cultrex Stem Cell Qualified Reduced Growth Factor Basement Membrane Extract include:
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Species: Human
Sample Types: Whole Cells
Applications: BioassayC Peron et al. (2020), Generation of a human iPSC line, FINCBi001-A, carrying a homoplasmic m.G3460A mutation in MT-ND1 associated with Leber�s Hereditary optic Neuropathy (LHON) Stem Cell Res, 48(0):101939.
PMID: 32771908 -
Species: Human
Sample Types: Whole Cells
Applications: BioassayGE Vázquez-Vé et al. (2019), Doublecortin like kinase 1 regulates alpha-Synuclein levels and toxicity J. Neurosci., 0(0).
PMID: 31748376
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FAQs
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What kinds of tumor cells or biopsy specimens grow in vivo with Cultrex® BME?
Many cell lines and tumor biopsy specimens (usually cut into small fragments) have been found to grow in vivo when implanted with Cultrex® BME. These include melanoma, intestinal, prostate, breast, lung, renal, and liver cancers as well as the 3T3 mouse embryonic fibroblast cell line.
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How does Cultrex® Basement Membrane Extract (BME) promote cell differentiation?
All epithelial and endothelial cells are in contact with a basement membrane matrix on at least one of their surfaces. By providing them with their natural matrix in vitro as a substrate for the cells that provides biological cues, the cells can assume a more physiological morphology (i.e. correct shape) and begin expression of cell-lineage specific proteins. Two-dimensional plastic surfaces, in combination with serum-containing media, cause cells to flatten, proliferate and de-differentiate.
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How should Cultrex Basement Membrane Extract (BME) be stored and handled?
Cultrex BME should be stored at or below -20°C for optimal stability. Preparation of working aliquots is recommended. Cultrex BME should be thawed overnight on ice at 4°C, however long term storage at 4°C is not recommended. Freeze/thaw cycles and gel-liquid phase transitions should be avoided, since they can compromise product integrity.
