Proteome Profiler Mouse Cytokine Array Kit, Panel A Best Seller
R&D Systems, part of Bio-Techne | Catalog # ARY006
Contains 4 membranes - each spotted in duplicate with 40 different cytokine antibodies
Key Product Details
Species
Mouse
Product Summary for Proteome Profiler Mouse Cytokine Array Kit, Panel A
Kit Summary
A membrane-based antibody array for the parallel determination of the relative levels of selected mouse cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and plasma.
Key Benefits
- Detects 40 mouse cytokines, chemokines, and acute phase proteins simultaneously
- Requires no specialized equipment
- Compatible with LI-COR* and chemiluminescence detection
Principle of the Assay
The Proteome Profiler Mouse Cytokine Array Kit, Panel A is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.
Why Use an Antibody Array to Detect Multiple Cytokines?
Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.
Kit Contents
- Rectangular 4-Well Multi-dish
- 4 Mouse Cytokine Array, Panel A nitrocellulose membranes spotted with 40 different antibodies to mouse cytokines, chemokines, and acute phase proteins
- Array Buffer 4
- Array Buffer 6
- Chemi Reagent 1
- Chemi Reagent 2
- Detection Antibody Cocktail, Mouse Cytokine Array, Panel A
- Streptavidin-HRP
- Transparency Overlay Template
- Wash Buffer Concentrate (25X)
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.
Stability and Storage
Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.
| Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample. | ||
|---|---|---|
| CXCL13/BLC/BCA-1 | IL-5 | M-CSF |
| C5a | IL-6 | CCL2/JE/MCP-1 |
| G-CSF | IL-7 | CCL12/MCP-5 |
| GM-CSF | IL-10 | CXCL9/MIG |
| CCL1/I-309 | IL-12 p70 | CCL3/MIP-1 alpha |
| CCL11/Eotaxin | IL-13 | CCL4/MIP-1 beta |
| ICAM-1 | IL-16 | CXCL2/MIP-2 |
| IFN-gamma | IL-17 | CCL5/RANTES |
| IL-1 alpha/IL-1F1 | IL-23 | CXCL12/SDF-1 |
| IL-1 beta/IL-1F2 | IL-27 | CCL17/TARC |
| IL-1ra/IL-1F3 | CXCL10/IP-10 | TIMP-1 |
| IL-2 | CXCL11/I-TAC | TNF-alpha |
| IL-3 | CXCL1/KC | TREM-1 |
| IL-4 | ||
Assays for Analytes represented in the Mouse Cytokine Array Kit, Panel A
| Analyte | Quantikine® ELISA Kits | DuoSet® ELISA Development Systems |
|---|---|---|
| CXCL13/BLC/BCA-1 | MCX130 | DY470 |
| C5a | DY2150 | |
| G-CSF | MCS00 | DY414 |
| GM-CSF | MGM00 | DY415 |
| CCL1/I-309/TCA-3 | DY845 | |
| CCL11/Eotaxin | MME00 | DY420 |
| ICAM-1 | MIC100 | DY796 |
| IFN-gamma | MIF00 | DY485 |
| IL-1 alpha | MLA00 | DY400 |
| IL-1 beta | MLB00B | DY401 |
| IL-1ra | MRA00 | DY480 |
| IL-2 | M2000 | DY402 |
| IL-3 | M3000 | DY403 |
| IL-4 | M4000B | DY404 |
| IL-5 | M5000 | DY405 |
| IL-6 | M6000B | DY406 |
| IL-7 | M7000 | DY407 |
| IL-10 | M1000b | DY417 |
| IL-12 p70 | M1270 | DY419 |
| IL-13 | M1300CB | DY413 |
| IL-16 | ||
| IL-17 | M1700 | DY421 |
| IL-23 | M2300 | DY1887 |
| IL-27 | M2728 | |
| CXCL10/IP-10/CRG-2 | MCX100 | DY466 |
| CXCL11/I-TAC | DY572 | |
| CXCL1/KC | MKC00B | DY453 |
| M-CSF | MMC00 | DY416 |
| CCL2/MCP-1/JE | MJE00 | DY479 |
| CCL12/MCP-5 | MCC120 | DY428 |
| CXCL9/MIG | MCX900 | DY492 |
| CCL3/MIP-1 alpha | MMA00 | DY450 |
| CCL4/MIP-1 beta | MMB00 | DY451 |
| CXCL2/MIP-2 | MM200 | DY452 |
| CCL5/RANTES | MMR00 | DY478 |
| CXCL12/SDF-1 | MCX120 | DY460 |
| CCL17/TARC | DY529 | |
| TIMP-1 | MTM100 | DY980 |
| TNF-alpha | MTA00 | DY410 |
| TREM-1 | MTRM10 | DY1187 |
Scientific Data Images for Proteome Profiler Mouse Cytokine Array Kit, Panel A
The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample.
Mouse splenocytes treated with 100 ng/mL LPS for 20 hours were mixed with a cocktail of biotinylated detection antibodies, and then incubated with the Mouse Cytokine Array. The array was then incubated with streptavidin-horseradish peroxidase followed by chemiluminescent detection. After detection, the array data were quantitated to generate a protein profile (histogram). The table shows the analytes detected and their location on the membrane.
The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates.
(A. and B.) Lysates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µg of lysate was used for each array shown. (C. and D.) Supernates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µL of supernate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.
The Mouse Cytokine Array detects multiple analytes in mouse serum and tissue lysates.
(A and B.) Samples of mouse serum from two different vendors. 200 µL of serum was used for each array shown. (C. and D.) Lysates of mouse lung tissue and mouse stomach tissue. 200 µg of lysate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.Formulation, Preparation, and Storage
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: Multiplex Cytokine Assay Kits
Cytokine Multiplex Assay Kits allow researchers to simultaneously detect the levels of multiple cytokines in a single sample. This is particularly useful for identifying the wide array of changes that can be associated with different pathological conditions. For multianalyte cytokine detection, we offer membrane-based Proteome Profiler Cytokine Antibody Arrays, bead-based Luminex® Assays, and Simple Plex automated ELISAs. For our antibody arrays, we offer human, mouse, or rat XL Cytokine Antibody Arrays that detect 105, 111, or 79 analytes respectively, including interleukins, interferons, chemokines, and members of the tumor necrosis factor superfamily. Our standard Luminex Assays for human, mouse, rat, porcine, and non-human primate (NHP) may also be used to detect many of these same analytes. With a menu of more than 490 analytes to select from, multiplexing with our Luminex assays provides the ability to simultaneously assess up to 50 analytes using magnetic microparticles. Experience consistent biomarker detection and ultra-sensitive results in less than 90 minutes with Ella, our automated benchtop ELISA. Simple Plex offers an extensive selection of over 250 fully validated assays essential to complete your cytokine research. Whether you want a stocked panel, ready to ship in just 2 days, a custom panel with up to 8 assays specific to your research, or a single assay cartridge for up to 72 samples ran simultaneously, Simple Plex has the solution to your research needs.
Additional Multiplex Cytokine Assay Kits Products
Product Documents for Proteome Profiler Mouse Cytokine Array Kit, Panel A
Product Specific Notices for Proteome Profiler Mouse Cytokine Array Kit, Panel A
For research use only
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