Mouse ACE-2 Antibody

Name: Mouse ACE-2 Antibody
Brand: R&D Systems
SKU: AF3437
Rating: 5 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF3437

Citations (9)

(2)

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Species Reactivity:	Mouse
Immunogen:	Chinese hamster ovary cell line CHO-derived recombinant mouse ACE-2 Gln18-Thr740 Accession # Q8R0I0
Applications:	ELISA, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects mouse ACE-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant rat ACE‑2 and 10% cross-reactivity with recombinant human ACE-2 is observed.

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Applications for Mouse ACE-2 Antibody

	Recommended Concentration	Sample
Western Blot	0.25 µg/mL	See below
Simple Western	2.5 µg/mL	See below
ELISA	This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse ACE‑2 Monoclonal Antibody (Catalog # MAB34371). This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Mouse ACE-2 DuoSet ELISA Kit (Catalog # DY3437-05) for convenient development of a sandwich ELISA.
Flow Cytometry	0.25 µg/10⁶ cells	HEK293 human embryonic kidney cell line transfected with mouse ACE-2 and eGFP
Immunohistochemistry	5-15 µg/mL	See below
Immunoprecipitation	25 µg/mL	Conditioned cell culture medium spiked with Recombinant Mouse ACE‑2 (Catalog # 3437‑ZN), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse ACE-2 Antibody

	Western Blot Detection of Mouse ACE‑2 by Western Blot. Western blot shows lysates of mouse kidney tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for ACE-2 at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Flow Cytometry Detection of ACE-2 in HEK293 Human Cell Line Transfected with Mouse ACE-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) mouse ACE-2 or (B) irrelevant protein, and eGFP was stained with Goat Anti-Mouse ACE-2 Affinity Purified Polyclonal Antibody (Catalog # AF3437) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). Quadrant markers were set based on Goat IgG control antibody (AB-108-C, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.
	Immunohistochemistry ACE‑2 in Mouse Kidney. ACE‑2 was detected in perfusion fixed frozen sections of mouse kidney using 15 µg/mL Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) overnight at 4 °C. Tissue was stained (red) and counterstained (green). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
	Immunohistochemistry ACE‑2 in Mouse Kidney. ACE-2 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
	Simple Western Detection of Mouse ACE‑2 by Simple Western^TM. Simple Western lane view shows lysates of mouse kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE-2 at approximately 141 kDa (as indicated) using 2.5 µg/mL of Goat Anti-Mouse ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
	ELISA Mouse ACE‑2 ELISA Standard Curve. Recombinant Mouse ACE‑2 protein was serially diluted 2-fold and captured by Rat Anti-Mouse ACE‑2 Monoclonal Antibody (MAB34371) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Mouse ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3437) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994).

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: ACE-2

ACE-2, also called ACEH (ACE homologue), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Mouse ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of mouse somatic ACE. The predicted mouse ACE-2 protein sequence consists of 798 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney and heart and at moderate levels in colon, small intestine and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3). In addition, ACE-2 is a key SARS-CoV Spike protein receptor in vivo and has a critical function in acute lung injury (4, 5).

Item	Value
References	Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238. Crackower, M.A. et al. (2002) Nature 417:822. Huang, L. et al. (2003) J. Biol. Chem. 278:15532. Kuba, K. et al. (2005) Nature Med. 11:875. Ima, Y. et al. (2005) Nature 436:112.
Long Name	Angiotensin I Converting Enzyme 2
Entrez Gene IDs	59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)
Alternate Names	ACE-2, ACE-related carboxypeptidase, ACE2, ACEH, ACEHangiotensin I converting enzyme 2, Angiotensin-converting enzyme homolog, DKFZp434A014, EC 3.4.17, EC 3.4.17.23, Metalloprotease MPROT15, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2, angiotensin-converting enzyme 2

Citations for Mouse ACE-2 Antibody (9)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse ACE-2 Antibody include:

Species: Transgenic Mouse
Sample Types: Whole Tissue
Applications: IHC

JR Enriquez et al. (2022), A dietary change to a high-fat diet initiates a rapid adaptation of the intestine Cell Reports, 41(7):111641.
PMID: 36384107
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

K Bilinska et al. (2021), Expression of the ACE2 virus entry protein in the nervus terminalis suggests an alternative route for brain infection in COVID-19 bioRxiv : the preprint server for biology, 0(0).
PMID: 33880469
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

P Wohlrab et al. (2021), Oxygen conditions oscillating between hypoxia and hyperoxia induce different effects in the pulmonary endothelium compared to constant oxygen conditions Physiological Reports, 9(3):e14590.
PMID: 33565273
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

S Kamle et al. (2021), Chitinase 3-like-1 is a Therapeutic Target That Mediates the Effects of Aging in COVID-19 bioRxiv : the preprint server for biology, 0(0).
PMID: 33442679
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

K Bilinska et al. (2021), Expression of the ACE2 Virus Entry Protein in the Nervus Terminalis Reveals the Potential for an Alternative Route to Brain Infection in COVID-19 Frontiers in Cellular Neuroscience, 15(0):674123.
PMID: 34290590
Species: Rat
Sample Types: Cell Lysates
Applications: Western Blot

J Jando et al. (2017), Expression and regulation of the neutral amino acid transporter B0AT1 in rat small intestine PLoS ONE, 12(9):e0184845.
PMID: 28915252
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Hashimoto T et al. (2012), ACE2 links amino acid malnutrition to microbial ecology and intestinal inflammation. Nature, 487(7408):477-81.
PMID: 22837003
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Singer D et al. (2009), Orphan transporter SLC6A18 is renal neutral amino acid transporter B0AT3. J. Biol. Chem., 284(30):19953-60.
PMID: 19478081
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Bilinska K et al. (0), Expression of the SARS-CoV-2 Entry Proteins, ACE2 and TMPRSS2, in Cells of the Olfactory Epithelium: Identification of Cell Types and Trends with Age. ACS Chem Neurosc11(11):1555-1562.
PMID: 32379417

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Customer Reviews for Mouse ACE-2 Antibody (2)

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Mouse ACE-2 Antibody

Name: Anonymous

Application: WB

Sample Tested: Heart tissue

Species: Mouse

Verified Customer | Posted 08/26/2016
Mouse ACE-2 Antibody

Name: Anonymous

Application: WB

Sample Tested: Adult kidney

Species: Mouse

Verified Customer | Posted 06/10/2016

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Mouse ACE-2 Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Mouse ACE-2 Antibody

Scientific Data Examples for Mouse ACE-2 Antibody

Western Blot

Flow Cytometry

Immunohistochemistry

Immunohistochemistry

Simple Western

ELISA

Formulation, Preparation and Storage

Reconstitution Calculator

Background: ACE-2

Citations for Mouse ACE-2 Antibody (9)

Customer Reviews for Mouse ACE-2 Antibody (2)

Customer Images

Mouse ACE-2 Antibody

Mouse ACE-2 Antibody

Product Documents for Mouse ACE-2 Antibody

Product Datasheets

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