Human OX40 Ligand/TNFSF4 Antibody

Name: Human OX40 Ligand/TNFSF4 Antibody
Brand: R&D Systems
SKU: MAB10541

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB10541

Citations (5)

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human OX40 Ligand Gln51-Leu183 Accession # P23510
Applications:	Block/Neutralize, CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 159403
Specificity:	Detects human OX40 Ligand in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhCD27 Ligand, recombinant mouse (rm) CD27 Ligand, rhCD30 Ligand, rmCD30 Ligand, rhCD40 Ligand, rmEDA, rhFas Ligand, rmFas Ligand, rhGITR Ligand, recombinant cotton rat TNF-alpha, rhTNF-alpha, rmTNF-alpha, recombinant porcine TNF-alpha, recombinant rat TNF-alpha, rhTRAIL, rhTRANCE, rmTRANCE, or rhVEGF is observed.

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Applications for Human OX40 Ligand/TNFSF4 Antibody

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/10⁶ cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Item	Value
Neutralization	Measured by its ability to neutralize OX40 Ligand/TNFSF4-induced IL-8 secretion in the HT1080 human fibrosarcoma cell line transfected with human OX40. Muller, N. et al. (2008) FEBS Journal 275:2296. The Neutralization Dose (ND₅₀) is typically 0.1-0.6 μg/mL in the presence of 10 ng/mL Recombinant Human OX40 Ligand/TNFSF4.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human OX40 Ligand/TNFSF4 Antibody

	Flow Cytometry Detection of OX40 Ligand/ TNFSF4 in Human Mature Dendritic Cells by Flow Cytometry. Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14⁺cells treated with 20 ng/mL Recombinant Human IL-4 (Catalog # 204-IL) and 50 ng/mL Recombinant Human GM-CSF (Catalog # 215-GM) for 7 days and 1 µg/mL LPS, 20 ng/mL Recombinant Human TNF-a (Catalog # 210-TA), and 20 ng/mL Recombinant Human IL-1 beta /IL-1F2 (Catalog # 201-LB) for last 24 hours were stained with Mouse Anti-Human OX40 Ligand/TNFSF4 Monoclonal Antibody(Catalog # MAB10541, filled histogram) or isotype control (Catalog # MAB002, open histogram), followed by Goat F(ab)2 Anti-Mouse IgG (H+L) Allophycocyanin (Catalog # F0101B).
	Immunocytochemistry OX40 Ligand/TNFSF4 in Human PBMCs. OX40 Ligand/TNFSF4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with LPS using Mouse Anti-Human OX40 Ligand/ TNFSF4 Monoclonal Antibody (Catalog # MAB10541) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (orange; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
	Neutralization IL‑8 Secretion Induced by OX40 Ligand/TNFSF4 and Neutralization by Human OX40 Ligand/TNFSF4 Antibody. Recombinant Human OX40 Ligand/TNFSF4 (Catalog # 1054-OX) induces IL-8 secretion in the HT1080 human fibrosarcoma cell line transfected with human OX40 in a dose-dependent manner (orange line) as measured by Human IL-8 Duoset (Catalog # DY208). IL-8 Secretion elicited by Recombinant Human OX40 Ligand/TNFSF4 (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human OX40 Ligand/TNFSF4 Monoclonal Antibody (Catalog # MAB10541). The ND₅₀ is typically 0.1-0.6 µg/mL.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: OX40 Ligand/TNFSF4

OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein designated TNFSF4 within the TNF superfamily. Human OX40L cDNA encodes a 183 amino acids (aa) polypeptide with an amino-terminal cytoplasmic domain (aa 1-23) and a carboxy-terminal extracellular domain (aa 51-183). It shares 46% aa sequence identity with the mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Like other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily that is expressed predominantly on activated CD4⁺ T cells. OX40 Ligand is one of the group of co-stimulatory molecules in the immune system that includes B7, CD40 Ligand, CD30 Ligand, CD27 Ligand and 4-1BB Ligand. OX40 appears as a late activation-induced T cell surface antigen, and its major function of OX40-OX40L interaction may be to transmit a late co-stimulatory signal to promote the survival and proliferation of activated CD4⁺ T cells and prolong the immune response. Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression.

Item	Value
References	Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757. Baum, P.R. et al. (1994) EMBO J. 13:3992. Al-Shamkhani, A. et al. (1997) J. Biol. Chem. 272:5275. Kjaergaard, J. et al. (2000) Cancer Res. 60:5514.
Entrez Gene IDs	7292 (Human); 22164 (Mouse); 89814 (Rat)
Alternate Names	CD134 ligand, CD134L, CD252, CD252 antigen, Glycoprotein Gp34, OX-40L, OX40 Ligand, OX40 antigen ligand, OX40L, OX4OL, TAX transcriptionally-activated glycoprotein 1, TNFSF4, TXGP1, gp34, tax-transcriptionally activated glycoprotein 1 (34kD), tax-transcriptionally activated glycoprotein 1, 34kD, tumor necrosis factor (ligand) superfamily, member 4, tumor necrosis factor ligand superfamily member 4

Citations for Human OX40 Ligand/TNFSF4 Antibody (5)

Showing 1 - 5 of 5 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human OX40 Ligand/TNFSF4 Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Neutralization

MMM Kaisar et al. (2018), Dectin-1/2-induced autocrine PGE2 signaling licenses dendritic cells to prime Th2 responses PLoS Biol., 16(4):e2005504.
PMID: 29668708
Species: Human
Sample Types: Whole Tissue
Applications: IHC

C Jacquemin et al. (2018), OX40L/OX40 axis impairs follicular and natural Treg function in human SLE JCI Insight, 3(24).
PMID: 30568041
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Beynon V et al. (2012), Activated Human CD4+CD45RO+ Memory T-Cells Indirectly Inhibit NLRP3 Inflammasome Activation through Downregulation of P2X7R Signalling. PLoS ONE, 7(6):e39576.
PMID: 22768094
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

(2012), B7-CD28 costimulatory signals control the survival and proliferation of murine and human gammadelta T cells via IL-2 production. J. Immunol., 189(3):1202-8.
PMID: 22732586
Species: Human
Sample Types: Cell Culture Supernates
Applications: Neutralization

Godfrey WR et al. (2005), Cord blood CD4(+)CD25(+)-derived T regulatory cell lines express FoxP3 protein and manifest potent suppressor function. Blood, 105(2):750-8.
PMID: 15374887

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Human OX40 Ligand/TNFSF4 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human OX40 Ligand/TNFSF4 Antibody

Scientific Data Examples for Human OX40 Ligand/TNFSF4 Antibody

Flow Cytometry

Immunocytochemistry

Neutralization

Formulation, Preparation and Storage

Reconstitution Calculator

Background: OX40 Ligand/TNFSF4

Citations for Human OX40 Ligand/TNFSF4 Antibody (5)

Product Documents for Human OX40 Ligand/TNFSF4 Antibody

Product Datasheets

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