Recombinant Human OX40 Ligand/TNFSF4 Protein, CF

Name: Recombinant Human OX40 Ligand/TNFSF4 Protein, CF
Brand: R&D Systems
SKU: 1054-OX
Rating: 5 (4 reviews)

Catalog # 1054-OX

Citations (6)

(4)

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Key Product Details

Source:	NS0
Accession #:	Q6FGS4
Applications:	Binding Activity, Bioactivity

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Product Specifications

Source

Mouse myeloma cell line, NS0-derived human OX40 Ligand/TNFSF4 protein

MHHHHHHHHHH	GGGSGGGSGGGS	IEGR	Human OX40 Ligand (Gln51-Leu183) Accession # Q6FGS4
N-terminus			C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Met

Predicted Molecular Mass

18 kDa

SDS-PAGE

28-34 kDa, reducing conditions

Activity

Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human OX40/TNFRSF4. Muller, N. et al. (2008) FEBS J. 275:2296. The ED₅₀ for this effect is typically 1-5 ng/mL. Measured by its binding ability in a functional ELISA. When Recombinant Human OX40/TNFRSF4 Fc Chimera (Catalog # 3388-OX) is immobilized at 10 ng/mL, the concentration of Recombinant Human OX40 Ligand/TNFSF4 that produces 50% optimal binding response is found to be approximately 0.5‑3 ng/mL.

Recombinant Human OX40 Ligand/TNFSF4 Protein, CF Scientific Data Examples

	Recombinant Human OX40 Ligand/TNFSF4 Protein Bioactivity Recombinant Human OX40 Ligand/TNFSF4 (Catalog # 1054-OX) induces IL-8 secretion in the HT1080 human fibrsarcoma cell line. The ED₅₀ for this effect is 1-5 ng/mL.
	Recombinant Human OX40 Ligand/TNFSF4 Protein SDS-PAGE 1 μg/lane of Recombinant Human OX40 Ligand/TNFSF4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing bands at 28-34 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1054-OX

Formulation	Supplied as a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: OX40 Ligand/TNFSF4

OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Human OX40L cDNA encodes a 183 amino acids (aa) polypeptide with an amino-terminal cytoplasmic domain (aa 1¬23) and a carboxy¬terminal extracellular domain (aa 51-183). It shares 46% aa sequence identity with the mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Similarly to other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40 Ligand is one of the co¬stimulatory molecules in the immune system that includes B7, CD40 Ligand, CD30 Ligand, CD27 Ligand and 4¬1BB Ligand. Because OX40 appears as a late activation-induced T cell surface antigen, it has been speculated that the major function of OX40-OX40L interaction is to transmit a late co-stimulatory signal to promote the survival and proliferation of activated CD4+ T cells and prolong the immune response. Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression.

Item	Value
References	Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757 Baum, P.R. et al. (1994) EMBO J. 13:3992. AlShamkhani, A. et al. (1997) J. Biol. Chem. 272:5275. Kjaergaard, J. et al. (2000) Cancer Res. 60:5514.
Entrez Gene IDs	7292 (Human); 22164 (Mouse); 89814 (Rat)
Alternate Names	CD134 ligand, CD134L, CD252, CD252 antigen, Glycoprotein Gp34, OX-40L, OX40 Ligand, OX40 antigen ligand, OX40L, OX4OL, TAX transcriptionally-activated glycoprotein 1, TNFSF4, TXGP1, gp34, tax-transcriptionally activated glycoprotein 1 (34kD), tax-transcriptionally activated glycoprotein 1, 34kD, tumor necrosis factor (ligand) superfamily, member 4, tumor necrosis factor ligand superfamily member 4

Citations for Recombinant Human OX40 Ligand/TNFSF4 Protein, CF (6)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human OX40 Ligand/TNFSF4 Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

LK Thampy et al. (2018), Restoration of T Cell function in multi-drug resistant bacterial sepsis after interleukin-7, anti-PD-L1, and OX-40 administration PLoS ONE, 13(6):e0199497.
PMID: 29944697
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

A Tanabe et al. (2018), Production and characterization of a novel site-specific-modifiable anti-OX40-receptor single-chain variable fragment for targeted drug delivery Biochem. Biophys. Res. Commun., 0(0).
PMID: 29330050
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance (SPR

AW Clarke et al. (2018), An anti-TL1A antibody for the treatment of asthma and inflammatory bowel disease MAbs, 0(0):1-43.
PMID: 29436901
Species: Human

Applications: Surface Plasmon Resonance

Lobito AA et al. (2011), Murine insulin growth factor-like (IGFL) and human IGFL1 proteins are induced in inflammatory skin conditions and bind to a novel tumor necrosis factor receptor family member, IGFLR1. J. Biol. Chem., 286(21):18969-81.
PMID: 21454693
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Yokoi T et al. (2008), Mycobacterium bovis Bacillus Calmette-Guerin suppresses inflammatory Th2 responses by inducing functional alteration of TSLP-activated dendritic cells. Int. Immunol., 20(10):1321-9.
PMID: 18703465
Species: Feline
Sample Types: Whole Cells
Applications: Bioassay

Willett BJ et al. (2006), Mapping the domains of CD134 as a functional receptor for feline immunodeficiency virus. J. Virol., 80(15):7744-7.
PMID: 16840353

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Customer Reviews (4)

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Recombinant Human OX40 Ligand/TNFSF4 Protein, CF

Name: Anonymous

Application: In vivo study

Verified Customer | Posted 03/25/2019
Recombinant Human OX40 Ligand/TNFSF4 Protein, CF

Name: Anonymous

Application: Cell migration/motility

Verified Customer | Posted 10/24/2018
Recombinant Human OX40 Ligand/TNFSF4 Protein, CF

Name: Anonymous

Application: SDS-PAGE Control

Verified Customer | Posted 10/12/2018
Recombinant Human OX40 Ligand/TNFSF4 Protein, CF

Name: Anonymous

Application: Cell Adhesion

Verified Customer | Posted 07/11/2018

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Recombinant Human OX40 Ligand/TNFSF4 Protein, CF

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Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications

Recombinant Human OX40 Ligand/TNFSF4 Protein, CF Scientific Data Examples

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: OX40 Ligand/TNFSF4

Citations for Recombinant Human OX40 Ligand/TNFSF4 Protein, CF (6)

Customer Reviews (4)

Recombinant Human OX40 Ligand/TNFSF4 Protein, CF

Recombinant Human OX40 Ligand/TNFSF4 Protein, CF

Recombinant Human OX40 Ligand/TNFSF4 Protein, CF

Recombinant Human OX40 Ligand/TNFSF4 Protein, CF

Product Documents

Product Datasheets

COA

Certificate of Analysis

SDS

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