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Recombinant Human OX40 Ligand/TNFSF4 Protein, CF Best Seller

Bio-Techne includes R&D Systems | Catalog # 1054-OX

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1054-OX-025

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Binding Activity, Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human OX40 Ligand/TNFSF4 protein
MHHHHHHHHHH GGGSGGGSGGGS IEGR Human OX40 Ligand
(Gln51-Leu183)
Accession # Q6FGS4
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

18 kDa

SDS-PAGE

28-34 kDa, reducing conditions

Activity

Measured by its ability to induce IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human OX40/TNFRSF4. Muller, N. et al. (2008) FEBS J. 275:2296.
The ED50 for this effect is typically 1-5 ng/mL.

Measured by its binding ability in a functional ELISA.
When Recombinant Human OX40/TNFRSF4 Fc Chimera (Catalog # 3388-OX) is immobilized at 10 ng/mL, the concentration of Recombinant Human OX40 Ligand/TNFSF4 that produces 50% optimal binding response is found to be approximately 0.5‑3 ng/mL.

Scientific Data Images for Recombinant Human OX40 Ligand/TNFSF4 Protein, CF

Recombinant Human OX40 Ligand/TNFSF4 Protein Bioactivity

Recombinant Human OX40 Ligand/TNFSF4 Protein Bioactivity

Recombinant Human OX40 Ligand/TNFSF4 (Catalog # 1054-OX) induces IL-8 secretion in the HT1080 human fibrsarcoma cell line. The ED50 for this effect is 1-5 ng/mL.
Recombinant Human OX40 Ligand/TNFSF4 Protein SDS-PAGE

Recombinant Human OX40 Ligand/TNFSF4 Protein SDS-PAGE

1 μg/lane of Recombinant Human OX40 Ligand/TNFSF4 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing bands at 28-34 kDa.

Formulation, Preparation and Storage

1054-OX
Formulation Supplied as a 0.2 μm filtered solution in PBS.
Reconstitution

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Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.

Background: OX40 Ligand/TNFSF4

OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein belonging to the TNF superfamily. Human OX40L cDNA encodes a 183 amino acids (aa) polypeptide with an amino-terminal cytoplasmic domain (aa 1¬23) and a carboxy¬terminal extracellular domain (aa 51-183). It shares 46% aa sequence identity with the mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Similarly to other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily that is expressed predominantly on activated CD4+ T cells. OX40 Ligand is one of the co¬stimulatory molecules in the immune system that includes B7, CD40 Ligand, CD30 Ligand, CD27 Ligand and 4¬1BB Ligand. Because OX40 appears as a late activation-induced T cell surface antigen, it has been speculated that the major function of OX40-OX40L interaction is to transmit a late co-stimulatory signal to promote the survival and proliferation of activated CD4+ T cells and prolong the immune response. Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression.

References

  1. Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757
  2. Baum, P.R. et al. (1994) EMBO J. 13:3992.
  3. Al­Shamkhani, A. et al. (1997) J. Biol. Chem. 272:5275.
  4. Kjaergaard, J. et al. (2000) Cancer Res. 60:5514.

Alternate Names

CD252, gp34, TNFSF4

Entrez Gene IDs

7292 (Human); 22164 (Mouse); 89814 (Rat)

Gene Symbol

TNFSF4

UniProt

Additional OX40 Ligand/TNFSF4 Products

Product Documents for Recombinant Human OX40 Ligand/TNFSF4 Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human OX40 Ligand/TNFSF4 Protein, CF

For research use only

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