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Recombinant Human IL-1 beta/IL-1F2 Protein Best Seller

Bio-Techne includes R&D Systems | Catalog # 201-LB

Analyzed by SEC-MALS
Catalog #
Availability
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Carrier Free
201-LB-005/CF
201-LB-010/CF
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201-LB-01M/CF
201-LB-025/CF
201-LB-100/CF
201-LB-500/CF
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Catalog #
Availability
Size / Price
Qty
With Carrier
201-LB-100
201-LB-025
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201-LB-010
201-LB-005
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Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human IL-1 beta/IL-1F2 protein
Ala117-Ser269

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala117 & Pro118

Predicted Molecular Mass

17 kDa

SDS-PAGE

17 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
The ED50 for this effect is <12 pg/mL.

Scientific Data Images for Recombinant Human IL-1 beta/IL-1F2 Protein

Recombinant Human IL‑1 beta/IL‑1F2 Protein SEC-MALS.

Recombinant Human IL-1beta Protein (Catalog # 201-LB) has a molecular weight (MW) of 17.0 kDa as analyzed by SEC-MALS, suggesting that this protein is a monomer.
Recombinant Human IL-1 beta/IL-1F2 Protein Bioactivity

Recombinant Human IL-1 beta/IL-1F2 Protein Bioactivity

Recombinant Human IL-1 beta/IL-1F2 (Catalog # 201-LB) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is <12 pg/mL.
Recombinant Human IL-1 beta/IL-1F2 Protein SDS-PAGE

Recombinant Human IL-1 beta/IL-1F2 Protein SDS-PAGE

1 μg/lane of Recombinant Human IL-1 beta/IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 201-LB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 50-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
*The 1 mg pack size (01M) can be stored for 1 month at 2 to 8 °C under sterile conditions after opening.
Carrier Free: 201-LB/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 50-200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage


Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 21% amino acid (aa) identity in human. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1-4). The human IL-1 beta cDNA encodes a 269 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta-converting enzyme (Caspase-1/ICE) to generate the active cytokine (5-7). The 17 kDa mature human IL-1 beta shares 96% aa sequence identity with rhesus and 67%-78% with canine, cotton rat, equine, feline, mouse, porcine, and rat IL-1 beta.

References

  1. Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. March, C.J. et al. (1985) Nature 315:641.
  6. Auron, P.E. et al. (1984) Proc. Natl. Acad. Sci. 81:7907.
  7. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

UniProt

Additional IL-1 beta/IL-1F2 Products

Product Documents for Recombinant Human IL-1 beta/IL-1F2 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-1 beta/IL-1F2 Protein

This product is covered by U.S. patent: 5,681,933.

For research use only

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