Human OX40 Ligand/TNFSF4 APC-conjugated Antibody

Name: Human OX40 Ligand/TNFSF4 APC-conjugated Antibody
Brand: R&D Systems
SKU: FAB10541A
Rating: 4 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB10541A

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human OX40 Ligand Gln51-Leu183 Accession # P23510
Applications:	Flow Cytometry
Label:	Allophycocyanin (Excitation=620-650 nm, Emission=660-670 nm)
Source:	Monoclonal Mouse IgG₁ Clone # 159403
Specificity:	Detects human OX40 Ligand in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) APRIL, rhBAFF, rhCD27 Ligand, recombinant mouse (rm) CD27 Ligand, rhCD30 Ligand, rmCD30 Ligand, rhCD40 Ligand, rmEDA, rhFas Ligand, rmFas Ligand, rhGITR Ligand, recombinant cotton rat TNF-alpha, rhTNF-alpha, rmTNF-alpha, recombinant porcine TNF-alpha, recombinant rat TNF-alpha, rhTRAIL, rhTRANCE, rmTRANCE, or rhVEGF is observed.

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Applications for Human OX40 Ligand/TNFSF4 APC-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human OX40 Ligand/TNFSF4 APC-conjugated Antibody

	Flow Cytometry Detection of OX40 Ligand/TNFSF4 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with Recombinant Human GM-CSF (Catalog # 215-GM) and LPS were stained with Mouse Anti-Human OX40 Ligand/TNFSF4 APC-conjugated Monoclonal Antibody (Catalog # FAB10541A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Background: OX40 Ligand/TNFSF4

OX40 Ligand (OX40L), also known as gp34, is a type II transmembrane glycoprotein designated TNFSF4 within the TNF superfamily. Human OX40L cDNA encodes a 183 amino acids (aa) polypeptide with an amino-terminal cytoplasmic domain (aa 1-23) and a carboxy-terminal extracellular domain (aa 51-183). It shares 46% aa sequence identity with the mouse counterpart. OX40L is expressed on the surface of activated B cells, T cells, dendritic cells and endothelial cells. Like other TNF superfamily members, membrane-bound OX40 Ligand exists as a homotrimer. OX40L binds to OX40 (CD134), a member of the TNF receptor superfamily that is expressed predominantly on activated CD4⁺ T cells. OX40 Ligand is one of the group of co-stimulatory molecules in the immune system that includes B7, CD40 Ligand, CD30 Ligand, CD27 Ligand and 4-1BB Ligand. OX40 appears as a late activation-induced T cell surface antigen, and its major function of OX40-OX40L interaction may be to transmit a late co-stimulatory signal to promote the survival and proliferation of activated CD4⁺ T cells and prolong the immune response. Engagement of OX40 on activated T cells in situ in tumors has been shown to augment immune responses and subsequent tumor regression.

Item	Value
References	Godfrey, W.R. et al. (1994) J. Exp. Med. 180:757. Baum, P.R. et al. (1994) EMBO J. 13:3992. Al-Shamkhani, A. et al. (1997) J. Biol. Chem. 272:5275. Kjaergaard, J. et al. (2000) Cancer Res. 60:5514.
Entrez Gene IDs	7292 (Human); 22164 (Mouse); 89814 (Rat)
Alternate Names	CD134 ligand, CD134L, CD252, CD252 antigen, Glycoprotein Gp34, OX-40L, OX40 Ligand, OX40 antigen ligand, OX40L, OX4OL, TAX transcriptionally-activated glycoprotein 1, TNFSF4, TXGP1, gp34, tax-transcriptionally activated glycoprotein 1 (34kD), tax-transcriptionally activated glycoprotein 1, 34kD, tumor necrosis factor (ligand) superfamily, member 4, tumor necrosis factor ligand superfamily member 4

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Human OX40 Ligand/TNFSF4 APC‑conjugated Antibody

Name: Anonymous

Application: Flow

Sample Tested: Peripheral blood cells

Species: Human

Verified Customer | Posted 10/26/2015

Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion. Buffer: 2% human serum, 0.5 mM EDTA in PBSDilution: 1/100

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Human OX40 Ligand/TNFSF4 APC-conjugated Antibody

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Applications for Human OX40 Ligand/TNFSF4 APC-conjugated Antibody

Scientific Data Examples for Human OX40 Ligand/TNFSF4 APC-conjugated Antibody

Flow Cytometry

Formulation, Preparation and Storage

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Background: OX40 Ligand/TNFSF4

Citations for Human OX40 Ligand/TNFSF4 APC-conjugated Antibody

Customer Reviews for Human OX40 Ligand/TNFSF4 APC-conjugated Antibody (1)

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Human OX40 Ligand/TNFSF4 APC‑conjugated Antibody

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