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Recombinant Human IL-4 Protein

Catalog # 204-IL | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
204-IL-001MG/CF
204-IL-010/CF
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204-IL-020/CF
204-IL-050/CF
204-IL-100/CF
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Catalog #
Availability
Size / Price
Qty
With Carrier
204-IL-010
204-IL-020
Show More Sizes
204-IL-050
204-IL-100
204-IL-500
Show Fewer Sizes

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human IL-4 protein
His25-Ser153, with an N-terminal Met

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

15 kDa

SDS-PAGE

14 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 0.0500-0.200 ng/mL.

Scientific Data Images for Recombinant Human IL-4 Protein

Recombinant Human IL-4 Protein Bioactivity Comparison.

As an alternative, please consider our next generation Recombinant Human IL-4 (BT-004). It has equivalent bioactivity to Recombinant Human IL-4 (Catalog # 204-IL). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human IL-4 proteins are measured in a cell proliferation assay using TF-1 human erythroleukemic cell line.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 204-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100-200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 204-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100-200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13 kDa‑18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1‑3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha-helix structure (4). Human IL-4 is synthesized with a 24 aa signal sequence. Alternate splicing generates an isoform with a 16 aa internal deletion. Mature human IL-4 shares 55%, 39% and 43% aa sequence identity with bovine, mouse, and rat IL-4, respectively. Human, mouse, and rat IL-4 are species-specific in their activities (5‑7). IL-4 exerts its effects through two receptor complexes (8, 9). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, -15, and ‑21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL‑13 R alpha1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgG4 and IgE in human B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (10‑13). IL-4 plays a dominant role in the development of allergic inflammation and asthma (12, 14).

References

  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. 83:5894.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Ramirez, F. et al. (1988) J. Immunol. Meth. 221:141.
  6. Leitenberg, D. and T.L. Feldbush (1988) Cell. Immunol. 111:451.
  7. Mosman, T.R. et al. (1987) J. Immunol. 138:1813.
  8. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  9. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  10. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  11. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  12. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  13. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  14. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.

Long Name

Interleukin 4

Alternate Names

BCDF, BCGF-1, BCGF1, BSF-1, BSF1, IL4, IL4E12

Entrez Gene IDs

3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)

Gene Symbol

IL4

UniProt

Product Documents for Recombinant Human IL-4 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IL-4 Protein

For research use only

Citations for Recombinant Human IL-4 Protein (390)

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Customer Reviews for Recombinant Human IL-4 Protein (23)

4.6 out of 5
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  • Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 10/04/2021
  • Recombinant Human IL-4 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 05/21/2021
  • Recombinant Human IL-4 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 05/05/2021
    We used IL4 to differentiate THP cells to Dendritic cells.
    Recombinant Human IL-4 Protein 204-IL
  • Recombinant Human IL-4 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 04/08/2021
    Recombinant Human IL-4 Protein 204-IL
  • Recombinant Human IL-4 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 11/23/2020
    Fast DC protocol. 24 hour GM-CSF + IL-4
  • Recombinant Human IL-4 Protein
    Name: Zhenping Wang
    Application: Differentiate DC cells.
    Verified Customer | Posted 09/30/2020
    Recombinant Human IL-4 Protein 204-IL
  • Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 07/07/2020
    Recombinant Human IL-4 Protein 204-IL
  • Recombinant Human IL-4 Protein
    Name: Jeff Cram
    Application: Cell Proliferation
    Verified Customer | Posted 06/20/2020
    Recombinant Human IL-4 Protein 204-IL
  • Recombinant Human IL-4 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 01/10/2020
  • Recombinant Human IL-4 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 07/26/2019
  • Recombinant Human IL-4 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/09/2019
    Cells supplied with 20ng/ml IL-4 and 100ng/ml of GM-CSF to differentiate dendritic cells
    Recombinant Human IL-4 Protein 204-IL
  • Recombinant Human IL-4 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/09/2019
    Recombinant Human IL-4 Protein 204-IL
  • Recombinant Human IL-4 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 11/16/2018
    Recombinant Human IL-4 Protein 204-IL
  • Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 10/08/2018
  • Recombinant Human IL-4 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 10/07/2018
    Cells were supplied with the cytokine treatments: 50 ng /ml GM-CSF and 10 ng/ ml1 of IL-4 to differentiate DC cells
    Recombinant Human IL-4 Protein 204-IL
  • Name: Mehdi Alavi
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 05/14/2018
  • Recombinant Human IL-4 Protein
    Name: Anonymous
    Application: stimulation of naive macrophages
    Verified Customer | Posted 01/12/2018
    stimulation of naive macrophages to differentiate then into M2 macrophages. Mannose receptor expression was used as a marker. Cells were stimulated with 10ng/ml IL-4and 10ng/ml IL-13 also from RnD systems
    Recombinant Human IL-4 Protein 204-IL
  • Recombinant Human IL-4 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 07/17/2017
    Used to prepare DCs from monocytes
  • Recombinant Human IL-4 Protein
    Name: Anonymous
    Application: differentiation of CD11c+ DCs from PBMC derived CD14+ monocytes
    Verified Customer | Posted 06/12/2017
    Used along with GMCSF for differentiation of CD11c+ DCs from PBMC derived CD14+ monocytes
  • Recombinant Human IL-4 Protein
    Name: jacqueline lee
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 08/09/2016
    Recombinant Human IL-4 Protein 204-IL
  • Recombinant Human IL-4 Protein
    Name: Anonymous
    Application: Cell Proliferation
    Verified Customer | Posted 05/16/2016
  • Recombinant Human IL-4 Protein
    Name: SOjan Abraham
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/18/2016
    Recombinant Human IL-4 Protein 204-IL
  • Recombinant Human IL-4 Protein
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 01/15/2016

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