Human IL-6R alpha Antibody

Name: Human IL-6R alpha Antibody
Brand: R&D Systems
SKU: MAB227
Rating: 5 (4 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB227

Citations (21)

(4)

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Key Product Details

Species Reactivity:	Human
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-6R alpha Leu20-Asp339 Accession # P08887
Applications:	Block/Neutralize, CyTOF-ready, ELISA Capture, Flow Cytometry, Western Blot
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 17506
Specificity:	Detects human IL-6R alpha in ELISAs and Western blots. In ELISAs, no cross-reactivity or interference was observed with recombinant human (rh) IL-1 alpha, recombinant mouse (rm) IL-1 alpha, rhIL-1 beta, rmIL-1 beta, rhIL-1ra, rhIL-2, rhIL-3, rmIL-3, rhIL-4, rmIL-4, rhIL-5, rmIL-5, rhIL-6, rmIL-6, rhIL-7, rmIL-7, rhIL-8, rhIL-9, rmIL-9, rhIL-10, or rhIL-11.

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Applications for Human IL-6R alpha Antibody

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Flow Cytometry	0.25 µg/10⁶ cells	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Human IL-6R alpha Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human IL-6R alpha Biotinylated Antibody (Catalog # BAF227)

Standard: Recombinant Human IL-6R alpha Protein (Catalog # 227-SR)

Item	Value
Neutralization	Measured by its ability to neutralize IL-6R alpha-mediated enhancement of IL‑6-dependent Inhibition of cell proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND₅₀) is typically 0.04-0.2 µg/mL in the presence of 30 ng/mL Recombinant Human IL-6R alpha and 30 ng/mL Recombinant Human IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-6R alpha Antibody

	Western Blot Detection of Recombinant Human IL-6R alpha by Western Blot. Western blot shows 25 ng of Recombinant Human IL-6R alpha (Catalog # 227-SR) and Recombinant Mouse IL-6R alpha (Catalog # 1830-SR). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for IL-6R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
	Flow Cytometry Detection of IL-6R alpha in U937 Human Cell Line by Flow Cytometry. U937 human histiocytic lymphoma cell line was labeled with Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B).
	Neutralization IL-6R alpha Enhancement of IL‑6-dependent Inhibition of Cell Proliferation and Neutralization by Human IL-6R alpha Antibody. Recombinant Human IL-6R alpha (Catalog # 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant HumanIL-6R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-6R alpha Monoclonal Antibody (Catalog # MAB227). The ND₅₀ is typically 0.04-0.2 µg/mL.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-6R alpha

The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha and is about 50kDa in size. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

Item	Value
References	Yamasaki et al. (1988) Science 241:825. Baumann et al. (1990) J. Biol. Chem. 265:19853. Hibi et al. (1990) Cell 63:1149. Schooltink et al. (1991) Eur. J. Biochem. 277:659. Novick et al. (1989) J. Exp. Med. 170:1409. Yasukawa et al. (1990) J. Biochem. 108:673. Saito et al. (1991) J. Immunology 147:168.
Long Name	Interleukin 6 Receptor alpha
Entrez Gene IDs	3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)
Alternate Names	CD126, IL-6 R alpha, IL-6R alpha, IL-6R-1, IL-6Ra, IL6Q, IL6R, IL6R alpha, IL6RA, IL6RQ, gp80, interleukin 6 receptor

Citations for Human IL-6R alpha Antibody (21)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-6R alpha Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Neutralization

S Sugahara et al. (2022), Peficitinib improves bone fragility by recovering bone turnover imbalance in arthritic mice Journal of pharmacological sciences, 148(1):134-141.
PMID: 34924117
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

S Shankar et al. (2022), Ex vivo-expanded human CD19+TIM-1+ regulatory B cells suppress immune responses in vivo and are dependent upon the TIM-1/STAT3 axis Nature Communications, 13(1):3121.
PMID: 35660734
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

E Kim et al. (2021), TM4SF5-dependent crosstalk between hepatocytes and macrophages to reprogram the inflammatory environment Cell Reports, 37(7):110018.
PMID: 34788612
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

C Rolvering et al. (2018), The PD-L1- and IL6-mediated dampening of the IL27/STAT1 anticancer responses are prevented by ?-PD-L1 or ?-IL6 antibodies J. Leukoc. Biol., 0(0).
PMID: 30040142
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

(2016), IL-6 trans-signaling is another pathway to upregulate Osteopontin Cytokine, 90(0):88-95.
PMID: 27863335
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

(2016), Comparative Ability of Mesenchymal Stromal Cells from Different Tissues to Limit Neutrophil Recruitment to Inflamed Endothelium PLoS ONE, 11(5):e0155161.
PMID: 27171357
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Resheq Y et al. (2015), Contact-dependent depletion of hydrogen peroxide by catalase is a novel mechanism of myeloid-derived suppressor cell induction operating in human hepatic stellate cells. J Immunol, 194(6):2578-86.
PMID: 25667417
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Sommer A et al. (2015), Vitamin D regulates cytokine patterns secreted by dendritic cells to promote differentiation of IL-22-producing T cells. PLoS ONE, 10(6):e0130395.
PMID: 26107738
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Becker et al. (2014), Mutant B-RAF-Mcl-1 survival signaling depends on the STAT3 transcription factor. Oncogene, 33(9):1158-66.
PMID: 23455323
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Zielinski CE et al. (2012), Pathogen-induced human TH17 cells produce IFN-gamma or IL-10 and are regulated by IL-1beta. Nature, 484(7395):514-8.
PMID: 22466287
Species: Human
Sample Types: In Vivo
Applications: In Vivo

Nieman KM et al. (2011), Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth. Nat. Med., 17(11):1498-503.
PMID: 22037646
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Kolliputi N et al. (2009), IL-6 cytoprotection in hyperoxic acute lung injury occurs via suppressor of cytokine signaling-1-induced apoptosis signal-regulating kinase-1 degradation. Am. J. Respir. Cell Mol. Biol., 40(3):314-24.
PMID: 18776134
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Ara T et al. (2009), Interleukin-6 in the bone marrow microenvironment promotes the growth and survival of neuroblastoma cells. Cancer Res., 69(1):329-37.
PMID: 19118018
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Duhen T et al. (2009), Production of interleukin 22 but not interleukin 17 by a subset of human skin-homing memory T cells. Nat. Immunol., 10(8):857-63.
PMID: 19578369
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Acosta-Rodriguez EV et al. (2007), Interleukins 1beta and 6 but not transforming growth factor-beta are essential for the differentiation of interleukin 17-producing human T helper cells. Nat. Immunol., 8(9):942-9.
PMID: 17676045
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Ammit AJ et al. (2007), Effect of IL-6 trans-signaling on the pro-remodeling phenotype of airway smooth muscle. Am. J. Physiol. Lung Cell Mol. Physiol., 292(1):L199-206.
PMID: 16936245
Species: Human
Sample Types: Plasma
Applications: ELISA Development

Mozaffarian D et al. (2004), trans fatty acids and systemic inflammation in heart failure. Am. J. Clin. Nutr., 80(6):1521-5.
PMID: 15585763
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Erices A et al. (2002), Gp130 activation by soluble interleukin-6 receptor/interleukin-6 enhances osteoblastic differentiation of human bone marrow-derived mesenchymal stem cells. Exp. Cell Res., 280(1):24-32.
PMID: 12372336
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Hurst SM et al. (2002), Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. J. Immunol., 169(9):5244-51.
PMID: 12391243
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Hurst SM et al. (2001), Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation. Immunity, 14(6):705-14.
PMID: 11420041
Species: Human
Sample Types: Cell Culture Supernates
Applications: Western Blot

Asou H et al. (1998), Mechanisms of growth control of Kaposi's sarcoma-associated herpes virus-associated primary effusion lymphoma cells. Blood, 91(7):2475-81.
PMID: 9516148

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Customer Reviews for Human IL-6R alpha Antibody (4)

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Human IL-6R alpha Antibody

Name: Katie Myers

Application: ELISA

Sample Tested: Serum

Species: Primate

Verified Customer | Posted 11/28/2022

Worked as an ELISA capture antibody
Human IL-6 R alpha Antibody

Name: Anonymous

Application: WB

Sample Tested: HepG2 human hepatocellular carcinoma cell line,Huh-7 human hepatoma cell line

Species: Human

Verified Customer | Posted 10/17/2018
Human IL-6 R alpha Antibody

Name: Anonymous

Application: ELISA

Sample Tested: EDTA Plasma

Species: Human

Verified Customer | Posted 05/31/2018

Paired with BAF227 this antibody was used to measure IL-6 receptor in human plasma samples using a sandwich immunoassay.
Human IL-6 R alpha Antibody

Name: Anonymous

Application: WB

Sample Tested: Stimulated Human Bronchial Epithelial Cell Line (BEAS-2B)

Species: Human

Verified Customer | Posted 11/29/2017

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Human IL-6R alpha Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Applications for Human IL-6R alpha Antibody

Human IL-6R alpha Sandwich Immunoassay

Scientific Data Examples for Human IL-6R alpha Antibody

Western Blot

Flow Cytometry

Neutralization

Formulation, Preparation and Storage

Reconstitution Calculator

Background: IL-6R alpha

Citations for Human IL-6R alpha Antibody (21)

Customer Reviews for Human IL-6R alpha Antibody (4)

Customer Images

Human IL-6R alpha Antibody

Human IL-6 R alpha Antibody

Human IL-6 R alpha Antibody

Human IL-6 R alpha Antibody

Product Documents for Human IL-6R alpha Antibody

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