Human IL-6R alpha Antibody

Name: Human IL-6R alpha Antibody
Brand: R&D Systems
SKU: AF-227-NA

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF-227-NA

Citations (5)

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Key Product Details

Species Reactivity:	Human
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-6R alpha Leu20-Asp358 Accession # P08887
Applications:	Block/Neutralize, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human IL-6R alpha in direct ELISAs and Western blots.

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Applications for Human IL-6R alpha Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	See below

Item	Value
Neutralization	Measured by its ability to neutralize IL-6R alpha-mediated inhibition of proliferation in the M1 mouse myeloid leukemia cell line. The Neutralization Dose (ND₅₀) is typically 0.2-1.6 µg/mL in the presence of 30 ng/mL Recombinant Human IL-6R alpha and 30 ng/mL Recombinant Human IL‑6.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-6R alpha Antibody

	Western Blot Detection of Recombinant Human IL-6R alpha by Western Blot. Western blot shows 5 ng of Recombinant Human IL-6R alpha (Catalog # 227-SR), Recombinant Mouse IL-6R alpha (Catalog # 1830-SR) and Recombinant Rat IL-6 R. PVDF Membrane was probed with 0.1 µg/mL of Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for IL-6R alpha at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
	Neutralization IL-6R alpha Enhancement of IL‑6‑dependent Inhibition of Cell Proliferation and Neutralization by Human IL-6R alpha Antibody. Recombinant Human IL-6R alpha (Catalog # 227-SR) enhances Recombinant Human IL-6 (Catalog # 206-IL) inhibition of proliferation in the M1 mouse myeloid leukemia cell line in a dose-dependent manner (orange line). Enhancement of Recombinant Human IL-6 (30 ng/mL) activity elicited by Recombinant Human IL-6R alpha (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-6R alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-227-NA). The ND₅₀ is typically 0.2-1.6 µg/mL.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-6R alpha

The multi-functional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha and is about 50kDa in size. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

Item	Value
References	Yamasaki et al. (1988) Science 241:825. Baumann et al. (1990) J. Biol. Chem. 265:19853. Hibi et al. (1990) Cell 63:1149. Schooltink et al. (1991) Eur. J. Biochem. 277:659. Novick et al. (1989) J. Exp. Med. 170:1409. Yasukawa et al. (1990) J. Biochem. 108:673. Saito et al. (1991) J. Immunology 147:168.
Long Name	Interleukin 6 Receptor alpha
Entrez Gene IDs	3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)
Alternate Names	CD126, IL-6 R alpha, IL-6R alpha, IL-6R-1, IL-6Ra, IL6Q, IL6R, IL6R alpha, IL6RA, IL6RQ, gp80, interleukin 6 receptor

Citations for Human IL-6R alpha Antibody (5)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-6R alpha Antibody include:

Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Salsano et al. (2013), An autoinflammatory neurological disease due to interleukin 6 hypersecretion. J Neuroinflammation, 10(0):29.
PMID: 23432807
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Cunin P et al. (2011), The tachykinins substance P and hemokinin-1 favor the generation of human memory Th17 cells by inducing IL-1beta, IL-23, and TNF-like 1A expression by monocytes. J. Immunol., 186(7):4175-82.
PMID: 21368235
Species: Human
Sample Types: Whole Cells
Applications: ICC

van Grevenstein WM et al. (2007), Inflammatory cytokines stimulate the adhesion of colon carcinoma cells to mesothelial monolayers. Dig. Dis. Sci., 52(10):2775-83.
PMID: 17394066
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot

Podewski EK et al. (2003), Alterations in Janus kinase (JAK)-signal transducers and activators of transcription (STAT) signaling in patients with end-stage dilated cardiomyopathy. Circulation, 107(6):798-802.
PMID: 12591746
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Pedersen IM et al. (2002), Protection of CLL B cells by a follicular dendritic cell line is dependent on induction of Mcl-1. Blood, 100(5):1795-801.
PMID: 12176902

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