Human IL-6R alpha Biotinylated Antibody

Name: Human IL-6R alpha Biotinylated Antibody
Brand: R&D Systems
SKU: BAF227
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # BAF227

Citations (8)

(1)

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Key Product Details

Species Reactivity:	Human
Immunogen:	S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL-6R alpha Leu20-Asp358 Accession # P08887
Applications:	ELISA Detection, Western Blot
Label:	Biotin
Source:	Polyclonal Goat IgG
Specificity:	Detects human IL-6R alpha in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) IL‑6, recombinant mouse IL-6, recombinant porcine IL-6, and rhgp130 is observed.

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Applications for Human IL-6R alpha Biotinylated Antibody

	Recommended Concentration	Sample
Western Blot	0.1 µg/mL	Recombinant Human IL-6R alpha (Catalog # 227-SR)

Human IL-6R alpha Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 µg/mL

Use in combination with:

Capture Reagent: Human IL-6R alpha Antibody (Catalog # MAB227R)

Capture Reagent: Human IL-6R alpha Antibody (Catalog # MAB227)

Standard: Recombinant Human IL-6R alpha Protein (Catalog # 227-SR)

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-6R alpha

The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80 kDa component receptor that binds IL-6 with low affinity (IL-6 R alpha ) and a signal-transducing component of 130 kDa (gp130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. Both components of the receptor complex, IL-6 R alpha and gp130 have been cloned, sequenced, and expressed (1-4).

A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans (5). This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alpha s have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130 (6, 7).

Item	Value
References	Yamasaki et al. (1988) Science 241:825. Baumann et al. (1990) J. Biol. Chem. 265:19853. Hibi et al. (1990) Cell 63:1149. Schooltink et al. (1991) Eur. J. Biochem. 277:659. Novick et al. (1989) J. Exp. Med. 170:1409. Yasukawa et al. (1990) J. Biochem. 108:673. Saito et al. (1991) J. Immunology 147:168.
Long Name	Interleukin 6 Receptor alpha
Entrez Gene IDs	3570 (Human); 16194 (Mouse); 24499 (Rat); 102116430 (Cynomolgus Monkey)
Alternate Names	CD126, IL-6 R alpha, IL-6R alpha, IL-6R-1, IL-6Ra, IL6Q, IL6R, IL6R alpha, IL6RA, IL6RQ, gp80, interleukin 6 receptor

Citations for Human IL-6R alpha Biotinylated Antibody (8)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-6R alpha Biotinylated Antibody include:

CM Flynn et al. (2021), Interleukin-6 controls recycling and degradation, but not internalization of its receptors The Journal of Biological Chemistry, 0(0):100434.
PMID: 33610555
Species: Mouse
Sample Types: Plasma
Applications: ELISA Capture

F Mimoto et al. (2020), Exploitation of Elevated Extracellular ATP to Specifically Direct Antibody to Tumor Microenvironment Cell Reports, 33(12):108542.
PMID: 33357423
Species: Human
Sample Types: Protein
Applications: ELISA Development

Iwayanagi Y et al. (2015), Inhibitory FcgammaRIIb-Mediated Soluble Antigen Clearance from Plasma by a pH-Dependent Antigen-Binding Antibody and Its Enhancement by Fc Engineering. J Immunol, 195(7):3198-205.
PMID: 26320252
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)

Dusterhoft S et al. (2014), A disintegrin and metalloprotease 17 dynamic interaction sequence, the sweet tooth for the human interleukin 6 receptor. J Biol Chem, 289(23):16336-48.
PMID: 24790088
Species: Canine
Sample Types: Whole Tissue
Applications: IHC-Fr

Wood MW et al. (2011), Autocrine effects of interleukin-6 mediate acute-phase proinflammatory and tissue-reparative transcriptional responses of canine bladder mucosa. Infect. Immun., 79(2):708-15.
PMID: 21115724
Species: Human
Sample Types: Plasma
Applications: ELISA Development

Mozaffarian D et al. (2004), trans fatty acids and systemic inflammation in heart failure. Am. J. Clin. Nutr., 80(6):1521-5.
PMID: 15585763
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Hurst SM et al. (2002), Secretion of oncostatin M by infiltrating neutrophils: regulation of IL-6 and chemokine expression in human mesothelial cells. J. Immunol., 169(9):5244-51.
PMID: 12391243
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development

Hurst SM et al. (2001), Il-6 and its soluble receptor orchestrate a temporal switch in the pattern of leukocyte recruitment seen during acute inflammation. Immunity, 14(6):705-14.
PMID: 11420041

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Human IL-6 R alpha Biotinylated Antibody

Name: Anonymous

Application: ELISA

Sample Tested: EDTA Plasma

Species: Human

Verified Customer | Posted 06/01/2018

Human IL-6 receptor alpha was measured in human plasma samples using an immunoassay comprising of this antibody and MAB227. This antibody was used as the detection and the MAB227 was used as the solid phase capture molecule.

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