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When expressing an antibody or recombinant protein in a heterologous host, getting to the right clone quickly is critical. Clone selection is the upstream stage of the bioprocessing workflow that identifies the production clone, or genetically identical stable cell line, that will generate your biologic. Thorough screening at the clone selection stage will help you select your best clone early in the process to save time and money downstream.

Whether you’re screening preliminary clones or identifying a production clone, our automated, high-throughput assay platforms let you quickly analyze complex cell culture samples for the highest yield of your biologic.

Cell Culture Screening

Jess Simple Western instrument by ProteinSimple, a Bio-Techne brand

Traditional techniques used to screen clones like the Western blot are labor-intensive, poorly reproducible, and only semiquantitative. Simple Western™ systems from ProteinSimple™ offer a powerful solution by coupling protein separation and sensitive detection in high-throughput. Separate and analyze proteins by either immunoassay or total protein content all in an automated fashion. You can also quantitate expression levels as well as fragmentation in a gel-free, blot-free format. Whether you’re screening preliminary clones or identifying a production clone, our automated, high-throughput assay platforms let you quickly analyze complex cell culture samples for the highest yield of your biologic.

Maurice iCE™ from ProteinSimple is a capillary electrophoresis platform that automates protein profiling by size or charge. Maurice streamlines cIEF and CE-SDS method development and data analysis for proteins, monoclonal antibodies, ADCs, and vaccines. 

Maurice: Automated cIEF and CE-SDS by ProteinSimple, a Bio-Techne brand

Cell Heterogeneity

Ensure that your preliminary and production clones are truly clonal. Confirm that the expression level of your product is consistent across cells in the culture.

Milo™ Single-Cell Western Assays analyze protein expression and identity in ~1000 individual cells in a single run. Analyze up to 12 proteins simultaneously to compare your product with other cellular proteins.

Bioprocessing Cell Heterogeneity Protein Expression with Milo