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iCE3 - Protein charge heterogeneity analysis in 10 minutes

Protein charge heterogeneity analysis in 10 minutes

iCE3™ lets you move beyond the limits of traditional protein analysis and fast-tracks your development timelines. 10-minute start to finish run times let you optimize method conditions in an afternoon. As an added bonus, you can use the same generic method for multiple molecules—no need for product-specific methods.

More Information About iCE3

Protein samples are first premixed with carrier ampholytes, additives and pI markers. Samples are separated in a capillary cartridge with electrolytic tanks at each end—one tank is filled with acid (anolyte) and the other base (catholyte).

The sample mixture is injected to fill the entire capillary column. Voltage is then applied to the anolyte and catholyte tanks. This creates a pH gradient, which separates and focuses the proteins based on their pI. The whole-column UV detector monitors the entire process in the capillary in real time. The focusing time can be optimized in a single sample run, and once it's complete the separation pattern is captured and analyzed. The capillary column is then washed so it's ready to go for the next sample.

How iCE works
Feature Description
Sample Volume/Run 12-17 µL
Sample Delivery Alcott 720 Autosampler or PrinCE Next Microinjector
Typical Run Time 10-15 minutes
Detection UV absorption at 280 nm
Focusing Voltage 600 V/cm
Dimensions 66 cm H x 28 cm W x 31 cm D
Weight 20 kg (45 lbs)
Power 120-230 V AC, 50/60 Hz