Sally Sue
So frugal, so accurate, so easy
Sally Sue Simple Western
The gel-free, blot-free, hands-free Simple Western is here! Sally Sue is an automated immunoassay using capillary electrophoresis. Sample loading, size-based separation, immunoprobing, washing, detection and quantitative data analysis are all done automatically, giving you a complete, walk-away solution. There are no messy gels, no transfer tanks or blots, and no film or imaging required. It’s just simple!
More Information About Sally Sue
Description | Total Protein Specification | Immunoassay Specification |
---|---|---|
Sample Required | 0.3-1.2 µg | 0.6-1.2 µg |
Volume Required | 3-5 µL/well | 3-5 µL/well |
Size Range |
Molecular weight (MW) ladders range from 2-440 kDa |
Molecular weight (MW) ladders range from 2-440 kDa |
Sizing CV | <10% | <10% |
Intra-assay CV | <15% | <15% |
Inter-assay CV | <20% | <20% |
Resolution (± percent difference in MW) |
± 15-20% for MW <20 kDa ±10 % for MW > 20 kDa |
± 15-20% for MW <20 kDa± 10 % for MW > 20 kDa |
Quantitation CV | <20% | <20% |
Dynamic Range | 3 logs | 3 logs |
Sensitivity | ng | Low pg |
Capillary | 5 cm, 100 µm, 400 nL |