Skip to main content
Sally Sue System - So frugal, so accurate, so easy
My sample is precious and I don't have enough for other methods
I have too many samples and need higher throughput protein characterization
My current method is not reproducible

So frugal, so accurate, so easy

Sally Sue™ lets you separate and analyze proteins by size from 2-440 kDa either by immunoassay or total protein. Got small sample volumes or starting materials? No problem. She uses as little as 0.2 µg/µL of protein in just 5 µL of sample, and runs up to 96 samples in one experiment. If you wish you could do more, Sally Sue gets you there!

Sally Sue Simple Western

The gel-free, blot-free, hands-free Simple Western is here! Sally Sue is an automated immunoassay using capillary electrophoresis. Sample loading, size-based separation, immunoprobing, washing, detection and quantitative data analysis are all done automatically, giving you a complete, walk-away solution. There are no messy gels, no transfer tanks or blots, and no film or imaging required. It’s just simple!

More Information About Sally Sue

Sample Required 0.3-1.2 µg 0.6-1.2 µg
Volume Required 3-5 µL/well 3-5 µL/well
Size Range Molecular weight (MW) ladders range
from 2-440 kDa
Molecular weight (MW) ladders range
from 2-440 kDa
Sizing CV <10% <10%
Intra-assay CV <15% <15%
Inter-assay CV <20% <20%
Resolution (± percent difference in MW) ± 15-20% for MW <20 kDa
±10 % for MW > 20 kDa
± 15-20% for MW <20 kDa±
10 % for MW > 20 kDa
Quantitation CV <20% <20%
Dynamic Range 3 logs 3 logs
Sensitivity ng Low pg
Capillary 5 cm, 100 µm, 400 nL