Sally Sue
So frugal, so accurate, so easy
Sally Sue Simple Western
The gel-free, blot-free, hands-free Simple Western is here! Sally Sue is an automated immunoassay using capillary electrophoresis. Sample loading, size-based separation, immunoprobing, washing, detection and quantitative data analysis are all done automatically, giving you a complete, walk-away solution. There are no messy gels, no transfer tanks or blots, and no film or imaging required. It’s just simple!
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My sample is precious and I don't have enough for other methodsSometimes you wish you could do more with your small and precious sample. Sally Sue, our most frugal system can run up to 96 data points in one overnight run with as little as 5 µL of sample. And she can utilize that sample to the max by allowing you to interrogate those few microliters eight times, such as when analyzing an entire cell signaling pathway in a single run. Imagine all the data you can get from your sample! |
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I have too many samples and need higher throughput protein characterizationOur Simple Western systems offer the right throughput for your needs. Sally Sue will process up to 96 data points per run in eight cycles of 12 data points each. Ideal for getting a lot of data in a single run. All the throughput you need with minimum hands-on time. |
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I am in a race to discover and I need my data fasterIf analyzing 96 data points overnight is not impressive enough, maybe we should add that it includes data analysis. Set up and start your run in the afternoon and come back the next morning to find molecular weight and signal quantification automatically calculated for each sample. If your time is as precious as your samples and you need results faster, we will help you win the race to discovery with Sally Sue. |
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My current method is not reproducibleHow reproducible will your current immunoassay be in analyzing 96 data points? With Sally Sue you get intra-assay CV less than 15% and inter-assay CV less than 20%. How's that for reproducibility? |
More Information About Sally Sue
| Description | Total Protein Specification | Immunoassay Specification |
|---|---|---|
| Sample Required | 0.3-1.2 µg | 0.6-1.2 µg |
| Volume Required | 3-5 µL/well | 3-5 µL/well |
| Size Range |
Molecular weight (MW) ladders range from 2-440 kDa |
Molecular weight (MW) ladders range from 2-440 kDa |
| Sizing CV | <10% | <10% |
| Intra-assay CV | <15% | <15% |
| Inter-assay CV | <20% | <20% |
| Resolution (± percent difference in MW) |
± 15-20% for MW <20 kDa ±10 % for MW > 20 kDa |
± 15-20% for MW <20 kDa± 10 % for MW > 20 kDa |
| Quantitation CV | <20% | <20% |
| Dynamic Range | 3 logs | 3 logs |
| Sensitivity | ng | Low pg |
| Capillary | 5 cm, 100 µm, 400 nL | |
