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Peggy Sue

Bio-Techne includes ProteinSimple | Catalog # 004-800

Peggy Sue System - Two ways to get separation, identification and quantitation
(3)
I need more than size data
I have too many samples and need higher throughput protein characterization

Two ways to get separation, identification and quantitation

Peggy Sue™ lets you separate and analyze proteins by size or charge from 2-440 kDa either by immunoassay or total protein. Got small sample volumes or starting materials? No problem. She uses as little as 0.2 µg/µL of protein in just 5 µL of sample, and runs up to 96 samples in one experiment. Charge ahead and do more with Peggy Sue!

Citations for Peggy Sue

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Testimonials from your Peers for Simple Western

Showing  1 - 5 of 32 testimonials Showing All

"Using Simple Western has been extremely rewarding, especially due to how easy it was to transfer our western blotting assays to a fully automated format on Simple Western and how well CST antibodies perform on the platform."

Chris LaBreck, Ph.D., Senior Product Scientist, Cell Signaling Technology (CST)

"Simple Western™ allows us to run three-hour assays, which would usually take us two days with traditional Western blots."

Laura Martins, Ph.D., Early Development Scientist, Oxford Biomedica

"Jess allows us to immediately identify potential autophagy biomarkers as they are upregulated or downregulated in response to treatment. This allows us to use more advanced statistical techniques such as principal component analysis and hierarchal clustering to recognize key correlations in our research with ease"

John K. Blackwood PhD, Head of Biology, and Jakub Stefaniak PhD, Samsara Therapeutics

"The introduction of an Abby will enable us to answer important questions quicker, such as identifying the disease driving mechanism and molecules surrounding osteoarthritis. Moving away from the tedious manual western blotting process, which can take up to two days, and introducing automation will speed up time to data and provide increased sensitivity and accuracy."

Dr. Mario Rothbauer, Postdoc Researcher, Department of Orthopedics and Trauma Surgery, Medical University of Vienna

Showing  1 - 5 of 32 testimonials Showing All

More Information About Peggy Sue

Peggy Sue Applications

Cancer & Immuno-Oncology

Cancer & Immuno-Oncology

Transform your Cancer & Immuno-oncology research by utilizing next-generation western blotting tools to give you richer views into your samples.

SARS-CoV-2

SARS-CoV-2

Reduce your lab time with automated simple western systems while accelerating your COVID-19 research. Set up, leave and remotely review your data in just 3 hours!

Cell & Gene Therapy

Cell & Gene Therapy

Next-generation analytical instrumentation to help improve and accelerate your cell and gene therapy workflows.

Targeted Protein Degradation

Targeted Degradation

Boost your screening studies using high throughput & hands-free Simple Western systems for quantitative analysis.

SARS-CoV-2 Serology

SARS-CoV-2 Serology

Quantitatively characterize serum or plasma human IgG antibodies reactive against 5 key viral antigens within one 3-hour run!

Exosome

Exosome

A validated workflow for protein analysis in extracellular vesicle samples from the leaders in exosome isolation and high sensitivity western blotting solutions.

Description Total Protein Specification Immunoassay Size Specification Immunoassay Charge Specification
Sample Required 0.3-1.2 µg lysate 0.6-1.2 µg 0.6-1.2 µg
Volume Required 3-5 µL/well 3-5 µL/well 5-12 µL/well
Size or pI Range Molecular weight (MW) ladders range from 2-440 kDa Molecular weight (MW) ladders range from 2-440 kDa Widest gradient ranges from pI 3 to pI 10
Sizing CV <10% <10% <10%
Intra-assay CV <15% <15% <20%
Inter-assay CV <20%** <20%* <20%
Resolution (± percent difference in MW) ± 15-20% for MW <20 kDa ±10 % for MW > 20 kDa ± 15-20% for MW <20 kDa ±10 % for MW > 20 kDa ± 0.1 pI units
Quantitation CV <20% <20% <20%
Dynamic Range 2-3 logs 3 logs 3 logs
Sensitivity ng Low pg Low pg
Capillary 5 cm, 100 µm, 400 nL
* Inter-assay CV is calculated with normalization to the system control protein
** Percent peak area