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Simple Westerns just got even simpler
Meet Wes, a protein characterization problem solver
Proteins are complex, but protein characterization shouldn't be. Are you struggling with methods that aren't quantitative? Are you spending all of your precious sample just to optimize your assay? Is the throughput inadequate? Are you waiting days to get your data? Watch this video to find out how Wes can be your protein characterization problem solver.
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I have too many samples and need higher throughput protein characterizationOur Simple Western systems offer the right throughput for your needs. Whether you are looking to process up to 24 samples per run or just 13, we have an instrument for you. Screen and optimize assays in less time. All the throughput you need with minimum hands-on time. |
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My sample is precious and I don't have enough for other methodsTraditional immunoassays often ask too much of your precious sample. You end up having to pool samples together or be content with getting just a few data points per experiment. With Wes you only need 3 µL of sample per capillary, so you can maximize the data you get per experiment. Imagine all the data you can get from your sample! |
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I need a method that is quantitativeWhether you want to do absolute or relative quantitation of your target, you need to reliably be able to detect signal saturation and work within the linear dynamic range of your assay. With HDR detection profile and analysis Wes gets you up to 6 logs of dynamic range. This means less time optimizing to get within the linear detection range of your assay. |
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I am in a race to discover and I need my data fasterTired of being handcuffed to your bench for hours tending to your gels and blots? Or maybe you are waiting in line for the dark room to free up? If your time is as precious as your samples and you need results faster, we will help you win the race to discovery with Wes. |
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My current method is not reproducibleAre your assays lacking the reproducibility and reliability that you need to be confident in your research? With Wes, you can achieve intra-assay CV less than 15%. How's that for reproducibility? |
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The environment matters to meMeet Jamie Boisvenue from the Department of Pediatrics, University of Alberta Faculty of Medicine & Dentistry. He found that traditional Western blot uses up to 300 pieces of plastic and Wes uses two. His lab joined the university's Office of Sustainability's Green Labs Program in 2013 that encourages labs across campus to be more environmentally friendly, and won the Campus Sustainability Leadership Award in 2015. |
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I'm frustrated with traditional Western blotsTraditional Westerns are as much an art form as they are a technical tool. They are rarely reproducible, they can take years to master and after you worked out the conditions they work beautifully until they don't, leaving you wondering what went wrong. Wes is fully automated, incredibly reproducible and gives you up to 25 data points in hours, not days. |
More Information About Wes
| Description | Total Protein Specification | Immunoassay Charge Specification |
|---|---|---|
| Sample Required | 0.3-1.2 µg lysate | 0.6-1.2 µg |
| Volume Required | 3 µL/well | 3 µL/well |
| Size or pI Range | Molecular weight (MW) ladders range from 2-440 kDa | Molecular weight (MW) ladders range from 2-440 kDa |
| Sizing CV | <10% | <10% |
| Intra-assay CV | <15% | <15% |
| Inter-assay CV | <20%* | <20%** |
| Resolution (± percent difference in MW) |
± 15-20% for MW <20 kDa ±10 % for MW > 20 kDa |
± 15-20% for MW <20 kDa ±10 % for MW > 20 kDa |
| Quantitation CV | <20% | <20% |
| Dynamic Range | 2-3 logs | 3 logs |
| Sensitivity | ng | Low pg |
| Capillary | 5 cm, 100 µm, 400 nL | |
* inter-assay CV is with system control
** percent peak area
