Mouse Dectin-1/CLEC7A Antibody

Name: Mouse Dectin-1/CLEC7A Antibody
Brand: R&D Systems
SKU: MAB17561
Rating: 3 (2 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB17561

Citations (4)

(2)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse Dectin‑1/CLEC7A Phe69-Leu244 Accession # Q6QLQ4
Applications:	Block/Neutralize, CyTOF-ready, Flow Cytometry
Label:	Unconjugated
Source:	Monoclonal Rat IgG_2A Clone # 218820
Specificity:	Detects mouse Dectin‑1/CLEC7A in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Dectin-1 is observed and no cross-reactivity with recombinant mouse Dectin-2 or rhDLEC is observed.

View Full Technical Details for Mouse Dectin-1/CLEC7A Antibody

Complete Your Research

Applications for Mouse Dectin-1/CLEC7A Antibody

	Recommended Concentration	Sample
Flow Cytometry	0.25 µg/10⁶ cells	RAW 264.7 mouse monocyte/macrophage cell line
Blockade of Receptor-ligand Interaction	In a functional ELISA, 0.05-0.25 µg/mL of this antibody will block 50% of the binding of 75 ng/mL of biotinylated Laminarin to immobilized Recombinant Mouse Dectin-1/CLEC7A (Catalog # 1756-DC) coated at 750 ng/mL (100 µL/well). At 3 μg/mL, this antibody will block >90% of the binding.
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse Dectin-1/CLEC7A Antibody

	Flow Cytometry Detection of Dectin‑1/CLEC7A in Raw264.7 Mouse Cell Line by Flow Cytometry. Mouse Raw264.7 monocyte/macrophage cell line was stained with Rat Anti-Mouse Dectin-1/CLEC7A Monoclonal Antibody (Catalog # MAB17561, filled histogram) or Rat IgG2A Isotype Control (MAB006, open histogram) followed by PE-conjugated anti-Rat IgG Secondary Antibody (F0105B). Staining was performed using our Staining Membrane-associated Proteins protocol.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.01 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 43 kDa type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner (1, 2). Mature mouse Dectin-1 is a 244 amino acid (aa) glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain (3). The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin‑1, respectively. It shares 25%‑34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region (4). Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells (5). It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (7). Yeast beta -glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection (15, 16).

Item	Value
References	Kanazawa, N. (2007) J. Dermatol. Sci. 45:77. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:20157. Heinsbroek, S.E.M. et al. (2006) J. Immunol. 176:5513. Taylor, P.R. et al. (2002) J. Immunol. 169:3876. Willment, J.A. et al. (2003) J. Immunol. 171:4569. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771. Gantner, B.N. et al. (2005) EMBO J. 24:1277. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119. Yadav, M. and J.S. Schorey (2006) Blood 108:3168. Suram, S. et al. (2006) J. Biol. Chem. 281:5506. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154. Saijo, S. et al. (2007) Nat. Immunol. 8:39. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Entrez Gene IDs	64581 (Human); 56644 (Mouse)
Alternate Names	BGR, Beta-glucan receptor, C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12, C-type lectin domain family 7 member A, C-type lectin domain family 7, member A, C-type lectin superfamily member 12, CD369, CLEC7A, CLECSF12, CLECSF12DC-associated C-type lectin 1, DECTIN1CANDF4, Dectin-1, Dectin1, Dendritic cell-associated C-type lectin 1, dendritic cell-associated C-type lectin-1, hDectin-1, lectin-like receptor 1

Citations for Mouse Dectin-1/CLEC7A Antibody (4)

Showing 1 - 4 of 4 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Mouse Dectin-1/CLEC7A Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

J Liu et al. (2019), Xylose rich heteroglycan from flaxseed gum mediates the immunostimulatory effects on macrophages via TLR2 activation Carbohydr Polym, 213(0):59-69.
PMID: 30879690
Species: Mouse
Sample Types: Whole Cells
Applications: Neutralization

(2015), Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans. PLoS ONE, 10(3):e0119244.
PMID: 25786219
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Yanez A et al. (2013), Detection of a TLR2 agonist by hematopoietic stem and progenitor cells impacts the function of the macrophages they produce. Eur J Immunol, 43(8):2114-25.
PMID: 23661549
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Ozment-Skelton TR et al. (2009), Leukocyte Dectin-1 expression is differentially regulated in fungal versus polymicrobial sepsis. Crit. Care Med., 37(3):1038-45.
PMID: 19237915

There are no citations that match your criteria.

Showing 1 - 4 of 4 citations Showing All

Customer Reviews for Mouse Dectin-1/CLEC7A Antibody (2)

2.5 out of 5

2 customer ratings

5 stars

4 stars

3 stars

50%

2 stars

50%

1 star

Customer Images

Showing 1 - 2 of 2 reviews Showing All

Filter By:

Mouse Dectin-1/CLEC7A Antibody

Name: Anonymous

Application: Flow

Sample Tested: Peripheral blood mononuclear cells (PBMCs)

Species: Mouse

Verified Customer | Posted 11/01/2021

Detection of Mouse Dectin-1 on mouse monocytes from PBMCs of C57BL/6 mouse strain. Mouse PBMC were gated on monocytes and Dectin-1 was detected with the Mouse Dectin-1 Antibody (catalog # MAB17561) (BLUE) or Rat IgG2A isotype control (RED), followed by a secondary Goat Anti-Rat IgG APC antibody.

Bio-Techne Response

Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.
Mouse Dectin-1/CLEC7A Antibody

Name: Anonymous

Application: Func

Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line

Species: Mouse

Verified Customer | Posted 07/11/2016

72 hours cytotoxicity assay with secondary Zap conjugate

There are no reviews that match your criteria.

Showing 1 - 2 of 2 reviews Showing All

Product Documents for Mouse Dectin-1/CLEC7A Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Mouse Dectin-1/CLEC7A Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Applications for Mouse Dectin-1/CLEC7A Antibody

Scientific Data Examples for Mouse Dectin-1/CLEC7A Antibody

Flow Cytometry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Dectin-1/CLEC7A

Citations for Mouse Dectin-1/CLEC7A Antibody (4)

Customer Reviews for Mouse Dectin-1/CLEC7A Antibody (2)

Customer Images

Mouse Dectin-1/CLEC7A Antibody

Mouse Dectin-1/CLEC7A Antibody

Product Documents for Mouse Dectin-1/CLEC7A Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected