Mouse Dectin-1/CLEC7A PE-conjugated Antibody

Name: Mouse Dectin-1/CLEC7A PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB17561P
Rating: 3 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB17561P

Citations (3)

(1)

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Species Reactivity:	Mouse
Immunogen:	Mouse myeloma cell line NS0-derived recombinant mouse Dectin‑1/CLEC7A Phe69-Leu244 Accession # Q6QLQ4
Applications:	Flow Cytometry
Label:	Phycoerythrin (Excitation=488 nm, Emission=565-605 nm)
Source:	Monoclonal Rat IgG_2A Clone # 218820
Specificity:	Detects mouse Dectin‑1/CLEC7A in direct ELISAs and Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) Dectin-1 is observed and no cross-reactivity with recombinant mouse Dectin-2 or rhDLEC is observed.

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Applications for Mouse Dectin-1/CLEC7A PE-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse Dectin-1/CLEC7A PE-conjugated Antibody

	Flow Cytometry Detection of Dectin‑1/CLEC7A in Raw264.7 Mouse Cell Line by Flow Cytometry. Mouse Raw264.7 monocyte/macrophage cell line was stained with Rat Anti-Mouse Dectin-1/CLEC7A PE-conjugated Monoclonal Antibody (Catalog # FAB17561P, filled histogram) or Rat IgG2A Isotype Control (IC006P, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.
	Flow Cytometry Detection of Dectin‑1/CLEC7A in Mouse Blood Monocytes by Flow Cytometry. Mouse whole blood monocytes were stained with Rat Anti-Mouse Gr-1/Ly-6G APC-conjugated Monoclonal Antibody (Catalog # FAB1037A) and either (A) Rat Anti-Mouse Dectin-1/CLEC7A PE-conjugated Monoclonal Antibody (Catalog # FAB17561P) or (B) Rat IgG_2APhycoerythrin Isotype Control (Catalog # IC006P). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

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Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 43 kDa type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner (1, 2). Mature mouse Dectin-1 is a 244 amino acid (aa) glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain (3). The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin‑1, respectively. It shares 25%‑34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region (4). Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells (5). It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (7). Yeast beta -glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8‑10). It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11‑13). Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection (15, 16).

Item	Value
References	Kanazawa, N. (2007) J. Dermatol. Sci. 45:77. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:20157. Heinsbroek, S.E.M. et al. (2006) J. Immunol. 176:5513. Taylor, P.R. et al. (2002) J. Immunol. 169:3876. Willment, J.A. et al. (2003) J. Immunol. 171:4569. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771. Gantner, B.N. et al. (2005) EMBO J. 24:1277. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119. Yadav, M. and J.S. Schorey (2006) Blood 108:3168. Suram, S. et al. (2006) J. Biol. Chem. 281:5506. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154. Saijo, S. et al. (2007) Nat. Immunol. 8:39. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Entrez Gene IDs	64581 (Human); 56644 (Mouse)
Alternate Names	BGR, Beta-glucan receptor, C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12, C-type lectin domain family 7 member A, C-type lectin domain family 7, member A, C-type lectin superfamily member 12, CD369, CLEC7A, CLECSF12, CLECSF12DC-associated C-type lectin 1, DECTIN1CANDF4, Dectin-1, Dectin1, Dendritic cell-associated C-type lectin 1, dendritic cell-associated C-type lectin-1, hDectin-1, lectin-like receptor 1

Citations for Mouse Dectin-1/CLEC7A PE-conjugated Antibody (3)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse Dectin-1/CLEC7A PE-conjugated Antibody include:

Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

JT Loh et al. (2019), Dok3-protein phosphatase 1 interaction attenuates Card9 signaling and neutrophil-dependent antifungal immunity J. Clin. Invest., 129(8):2717-2729.
PMID: 31180338
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

(2015), Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans. PLoS ONE, 10(3):e0119244.
PMID: 25786219
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

Xu S et al. (2009), Activated dectin-1 localizes to lipid raft microdomains for signaling and activation of phagocytosis and cytokine production in dendritic cells. J. Biol. Chem., 284(33):22005-11.
PMID: 19525229

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Mouse Dectin-1/CLEC7A PE-conjugated Antibody

Name: Anonymous

Application: Flow

Sample Tested: RAW 264.7 mouse monocyte/macrophage cell line

Species: Mouse

Verified Customer | Posted 07/11/2016

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Mouse Dectin-1/CLEC7A PE-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Applications for Mouse Dectin-1/CLEC7A PE-conjugated Antibody

Scientific Data Examples for Mouse Dectin-1/CLEC7A PE-conjugated Antibody

Flow Cytometry

Flow Cytometry

Formulation, Preparation and Storage

Plan Your Experiments

Reconstitution Calculator

Background: Dectin-1/CLEC7A

Citations for Mouse Dectin-1/CLEC7A PE-conjugated Antibody (3)

Customer Reviews for Mouse Dectin-1/CLEC7A PE-conjugated Antibody (1)

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Mouse Dectin-1/CLEC7A PE-conjugated Antibody

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