Recombinant Mouse Dectin-1/CLEC7A Protein, CF

Name: Recombinant Mouse Dectin-1/CLEC7A Protein, CF
Brand: R&D Systems
SKU: 1756-DC

Catalog # 1756-DC | R&D Systems, Inc. a Bio-Techne Brand

Citations (3)

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Key Product Details

Source:	NS0
Accession #:	Q6QLQ4
Applications:	Binding Activity

View Full Technical Details for Recombinant Mouse Dectin-1/CLEC7A Protein, CF

Technical Data
Citations (3) / Reviews

Product Specifications for Recombinant Mouse Dectin-1/CLEC7A Protein, CF

Source	Mouse myeloma cell line, NS0-derived mouse Dectin-1/CLEC7A protein Phe69-Leu244, with an N-terminal 10-His tag
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	His
Predicted Molecular Mass	21.6 kDa
SDS-PAGE	21.6 kDa
Activity	Measured by its ability to bind biotinylated Laminarin (1, 3-beta -glucan) in a functional ELISA with an estimated K_D <6 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1756-DC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Dectin-1/CLEC7A

Dectin-1, also known as CLEC7A and the beta -glucan receptor, is a 43 kDa type II transmembrane C-type lectin that functions in the innate immune response to fungal pathogens. Although Dectin-1 resembles other CLEC molecules structurally, it binds ligands in a calcium-independent manner (1, 2). Mature mouse Dectin-1 is a 244 amino acid (aa) glycoprotein that consists of a short ITAM-containing cytoplasmic tail, a transmembrane segment, and a stalk and carbohydrate recognition domain (CRD) in the extracellular domain (3). The CRD of mouse Dectin-1 shares 61%, 60%, and 87% aa sequence identity with that of bovine, human, and rat Dectin-1, respectively. It shares 25% - 34% aa sequence identity with the CRD of other subgroup members CLEC-1, CLEC-2, CLEC9A, CLEC12B, LOX-1, and MICL. Mouse Dectin-1 is alternately spliced, generating a variant that lacks the stalk region (4). Mouse Dectin-1 is expressed on monocytes, macrophages, and neutrophils, and on some populations of dendritic cells and T cells (5). It is upregulated on macrophages by GM-CSF, IL-4, or IL-13 and downregulated by dexamethasone, IL-10, or LPS (6). The CRD selectively binds beta -glucan polymers, a major component of yeast and mycobacterial cell walls (7). Yeast beta -glucan is accessible to Dectin-1 only at sites of cell budding, and Dectin-1 does not recognize the filamentous form of yeast (8). Dectin-1 mediates the phagocytosis of zymosan particles and intact yeast (8 - 10). It co-localizes with TLR2 in the presence of zymosan, and the two receptors cooperate in ligand recognition and the propagation of proinflammatory signaling (9, 11 - 13). Dectin-1 interaction with the tetraspanin CD37 increases its stability on the cell membrane and inhibits ligand-induced signaling (14). Genetic knockout of Dectin-1 in mice increases their susceptibility to pathogenic infection (15, 16).

Item	Value
References	Kanazawa, N. (2007) J. Dermatol. Sci. 45:77. Brown, G.D. (2006) Nat. Rev. Immunol. 6:33. Ariizumi, K. et al. (2000) J. Biol. Chem. 275:20157. Heinsbroek, S.E.M. et al. (2006) J. Immunol. 176:5513. Taylor, P.R. et al. (2002) J. Immunol. 169:3876. Willment, J.A. et al. (2003) J. Immunol. 171:4569. Palma, A.S. et al. (2006) J. Biol. Chem. 281:5771. Gantner, B.N. et al. (2005) EMBO J. 24:1277. Gantner, B.N. et al. (2003) J. Exp. Med. 197:1107. Kennedy, A.D. et al. (2007) Eur. J. Immunol. 37:467. Brown, G.D. et al. (2003) J. Exp. Med. 197:1119. Yadav, M. and J.S. Schorey (2006) Blood 108:3168. Suram, S. et al. (2006) J. Biol. Chem. 281:5506. Meyer-Wentrup, F. et al. (2007) J. Immunol. 178:154. Saijo, S. et al. (2007) Nat. Immunol. 8:39. Taylor, P.R. et al. (2007) Nat. Immunol. 8:31.
Entrez Gene IDs	64581 (Human); 56644 (Mouse)
Alternate Names	BGR, Beta-glucan receptor, C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 12, C-type lectin domain family 7 member A, C-type lectin domain family 7, member A, C-type lectin superfamily member 12, CD369, CLEC7A, CLECSF12, CLECSF12DC-associated C-type lectin 1, DECTIN1CANDF4, Dectin-1, Dectin1, Dendritic cell-associated C-type lectin 1, dendritic cell-associated C-type lectin-1, hDectin-1, lectin-like receptor 1

Citations for Recombinant Mouse Dectin-1/CLEC7A Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Dectin-1/CLEC7A Protein, CF include:

Species: Mouse
Sample Types: Recombinant Protein
Applications: Bioassay, Mass Spectrometry

C Leclaire et al. (2018-01-29), Molecular basis for intestinal mucin recognition by galectin-3 and C-type lectins FASEB J., 2018-00(0):fj201700619R.
PMID: 29401627
Sample Types: Complex Sample Type
Applications: Bioassay

AJ Smith et al. (2017-12-15), Immunoregulatory Activity of the Natural Product Laminarin Varies Widely as a Result of Its Physical Properties J. Immunol., 2017-10(0).
PMID: 29246954
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2015-03-18), Correction: A chitin-like component on sclerotic cells of fonsecaea pedrosoi inhibits dectin-1-mediated murine Th17 development by masking beta-glucans. PLoS ONE, 2015-010(3):e0119244.
PMID: 25786219

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