Human PD-L1/B7-H1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1562
Recombinant Monoclonal Antibody.
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Blockade of Receptor-ligand Interaction, Neutralization, Flow Cytometry, CyTOF-ready
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2340D
Product Specifications
Immunogen
Mouse myeloma cell line, NS0-derived human PD-L1/B7-H1 protein
Phe19-Thr239
Accession # Q9NZQ7
Phe19-Thr239
Accession # Q9NZQ7
Specificity
Detects human PD-L1/B7-H1 in direct ELISAs.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human PD-L1/B7-H1 Antibody
Detection of human PD-L1/B7-H1 by Western Blot.
Western blot shows lysates of human lung tissue. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1562) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PD-L1/B7-H1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of PD-L1/B7-H1 in HEK293 Human Cell Line Transfected with Human PD-L1/B7-H1 and eGFP by Flow Cytometry.
HEK293 human embryonic kidney cell line transfected with either (A) human PD-L1/B7-H1 or (B) irrelevant protein and eGFP was stained with Rabbit Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1562) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (F0111). Quadrant markers were set based on Rabbit IgG control antibody staining (MAB1050). View our protocol for Staining Membrane-associated Proteins.
PD-1 binding to Human PD-L1/B7-H1-transfected HEK293 Human Cell Line is Blocked by Human PD-L1/B7-H1 Antibody.
In a functional flow cytometry test, Recombinant Human PD-1 Fc Chimera Alexa Fluor® 647 protein (AFR1086, 500 ng/mL) binds to HEK293 human embryonic kidney cell line transfected with recombinant human PD-L1/B7-H1. (A) Binding is completely blocked by 25 µg/mL of Rabbit Anti-Human PD-L1/B7-H1 Monoclonal Antibody (Catalog # MAB1562) but not by (B) Rabbit IgG Isotype Control (MAB1050). View our protocol for Staining Membrane-associated Proteins.Applications for Human PD-L1/B7-H1 Antibody
Application
Recommended Usage
Blockade of Receptor-ligand Interaction
In a functional flow cytometry test, 2.5 μg/mL of Rabbit anti-human PD-L1/B7-H1 Antibody(Catalog # MAB1562) will block the binding of Recombinant Human PD-1 His-tagged protein (Catalog # 8986-PD) to HEK293 human embryonic kidney cell line transfected with recombinant human PD-L1/B7-H1.
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human PD-L1/B7-H1 and eGFP
Sample: HEK293 Human Cell Line Transfected with Human PD-L1/B7-H1 and eGFP
Western Blot
2 µg/mL
Sample: Human Lung Tissue
Sample: Human Lung Tissue
Neutralization
In a functional ELISA binding assay, 0.2-1.2 µg/mL of this
antibody will block 50% of the binding of 1 µg/mL of Recombinant Human
PD-L1/B7-H1 (Catalog # 156-B7) to immobilized Recombinant Human PD-1 Fc chimera (Catalog # 1086-PD) coated at 100 ng/mL (100 µL/well). At 2-3 µg/mL, this antibody
will block >90% of the binding.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PD-L1/B7-H1
inflammatory-activated immune cells including macrophages, T cells, and B cells (4-7), keratinocytes (8, 9), enothelial and intestinal epithelial cells (8, 10), as well as a variety of carcinomas and melanoma (11, 12). B7-H1 binds to T cell B7-1/CD80 and PD-1 (7, 8, 12-15). It suppresses T cell activation and proliferation (5, 8, 14, 16) and induces the apoptosis of activated T cells (11). It plays a role in the development of immune tolerance by promoting T cell anergy (7, 14) and enhancing regulatory T cell development (16). B7-H1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (5, 10) and inhibits the development of Th17 cells (16). In cancer, B7-H1 provides resistance to T cell mediated lysis, enhances EMT, and enhances the tumorigenic function of Th22 cells (6, 9, 12, 15).
References
- Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
- Dong, H. et al. (1999) Nat. Med. 5:1365.
- Frigola, X. et al. (2011) Clin. Cancer Res. 17:1915.
- Tamura, H. et al. (2001) Blood 97:1809.
- Chen, L. et al. (2007) J. Immunol. 178:6634.
- Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
- Tsushima, F. et al. (2007) Blood 110:180.
- Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
- Cao, Y. et al. (2010) Cancer Res. 71:1235.
- Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
- Dong, H. et al. (2002) Nat. Med. 8:793.
- Azuma, T. et al. (2008) Blood 111:3635.
- Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
- Park, J.-J. et al. (2010) Blood 116:1291.
- Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
- Herold, M. et al. (2015) J. Immunol. 195:3584.
Long Name
Programmed Death Ligand 1
Alternate Names
B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1
Entrez Gene IDs
Gene Symbol
CD274
UniProt
Additional PD-L1/B7-H1 Products
Product Documents for Human PD-L1/B7-H1 Antibody
Product Specific Notices for Human PD-L1/B7-H1 Antibody
For research use only
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