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Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF Best Seller

Bio-Techne includes R&D Systems | Catalog # 156-B7

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156-B7-01M
156-B7-100

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human PD-L1/B7-H1 protein
Human PD-L1
(Phe19-Thr239)
Accession # Q9NZQ7
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe19

Predicted Molecular Mass

52 kDa (monomer)

SDS-PAGE

70-75 kDa, reducing conditions

Activity

Measured by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocytes.
The ED50 for this effect is 0.075-0.75 μg/mL.

Scientific Data Images for Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF

Recombinant Human PD-L1/B7-H1 Fc Chimera Protein SEC-MALS.

Recombinant human B7-H1/Fc (Catalog # 156-B7) has a molecular weight (MW) of 135.0 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).
Graph showing bioactivity of Human PD-L1 protein (B7-H1 protein)

Bioactivity of PD-L1 Protein

Recombinant Human PD-L1 / B7-H1 Fc Chimera (Catalog # 156-B7) inhibits anti-CD3 antibody-induced IL-2 secretion in human T lymphocytes. The ED50 for this effect is 0.075-0.75 µg/mL in the presence of Goat Anti-Human IgG Fc Polyclonal Antibody (Catalog # G-102-C).

Formulation, Preparation and Storage

156-B7
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and NaCl.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-L1/B7-H1

PD-L1, also known as B7-H1, PDL1, is one of the ligands for PD-1 and plays a critical role in the regulation of T cell immunity (1-6). The PD-1:PD-L1 interaction initiates a negative signaling cascade in T cells leading to inhibition of T cell activation (2, 5, 7, 8). PD-L1 provides a molecular stop signal to the adaptive immune system helping to distinguish between self and foreign antigens. PD-L1 also plays a role in the development of immune tolerance by promoting T cell anergy (1, 5) and enhancing regulatory T cell development (8). In addition, PD-L1 favors the development of anti-inflammatory IL-10 and IL-22 producing dendritic cells (7, 9) and inhibits the development of Th17 cells (8). Many cancers exhibit upregulated PD-L1 protein expression, and several cancers with high levels of PD-L1 have been associated with increased tumor aggressiveness and poor prognosis. Using new therapeutics that block the PD-L1:PD-1 interaction has proven successful in the clinic for many cancer types and has sparked great interest in the field of cancer immunotherapy.

The PD-L1 protein is an approximately 65 kDa transmembrane glycoprotein belonging to the B7 family of immune regulatory molecules (10). Mature human PD-L1 protein consists of a 220 amino acid (aa) extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane segment, and a 31 aa cytoplasmic domain (11). Within the ECD, human PD-L1 shares 73% and 74% aa sequence identity with mouse and rat B7-H1, respectively. Alternative splicing generates additional isoforms that either lack the first Ig-like domain or are truncated within the second Ig-like domain (12). PD-L1 is expressed on inflammatory-activated immune cells including macrophages, T cells, and B cells (10, 13, 14, 16) keratinocytes (9, 11), endothelial and intestinal epithelial cells (2, 9), as well as a variety of carcinomas and melanoma (12, 16).

References

  1. Tsushima, F. et al. (2007) Blood 110:180.
  2. Mazanet, M.M. and C.C.W. Hughes (2002) J. Immunol. 169:3581.
  3. Azuma, T. et al. (2008) Blood 111:3635.
  4. Butte, M.J. et al. (2008) Mol. Immunol. 45:3567.
  5. Park, J.-J. et al. (2010) Blood 116:1291.
  6. Ritprajak, P. et al. (2010) J. Immunol. 184:4918.
  7. Chen, L. et al. (2007) J. Immunol. 178:6634.
  8. Herold, M. et al. (2015) J. Immunol. 195:3584.
  9. Scandiuzzi, L. et al. (2014) Cell Rep. 6:625.
  10. Ceeraz, S. et al. (2013) Trends Immunol. 34:556.
  11. Dong, H. et al. (1999) Nat. Med. 5:1365.
  12. Frigola, X. et al. (2011) Clin. Cancer Res. 17:1915.
  13. Tamura, H. et al. (2001) Blood 97:1809.
  14. Kuang, D.-M. et al. (2014) J. Clin. Invest. 124:4657.
  15. Cao, Y. et al. (2010) Cancer Res. 71:1235.
  16. Dong, H. et al. (2002) Nat. Med. 8:793.

Long Name

Programmed Death Ligand 1

Alternate Names

B7-H1, B7H1, CD274, PDCD1L1, PDCD1LG1, PDL1

Entrez Gene IDs

29126 (Human); 60533 (Mouse); 499342 (Rat); 574058 (Porcine); 484186 (Canine); 102145573 (Cynomolgus Monkey)

Gene Symbol

CD274

UniProt

Additional PD-L1/B7-H1 Products

Product Documents for Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF

For research use only

Citations for Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF (29)

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Customer Reviews for Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF (21)

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  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 06/19/2020
    Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF 156-B7
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Sarah Garrett
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/15/2019
    HEK cells expressing mCherry alone (LEFT) or hPD-1 mCherry (RIGHT) were incubated with 0.25 ug of hPD-L1 hIgG1. Binding was monitored by flow cytometry using an anti-human 488 secondary antibody.
    Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF 156-B7
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 12/06/2018
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Cell Adhesion
    Verified Customer | Posted 10/24/2018
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 10/12/2018
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Jenna Nguyen
    Application: Cell Proliferation
    Verified Customer | Posted 09/28/2018
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 08/26/2018
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 04/12/2018
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 03/30/2018
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 03/15/2018
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 03/11/2018
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 02/07/2018
    Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF 156-B7
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Amit Kumar
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 12/18/2017
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 12/06/2017
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Konrad Gabrusiewicz
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 12/01/2017
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/27/2017
    Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF 156-B7
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/18/2017
  • Name: Jenna Nguyen
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 07/20/2017
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Cell Adhesion
    Verified Customer | Posted 07/20/2017
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: SPR assay
    Verified Customer | Posted 07/14/2017
    SPR assay for Kinetics between PDL-1 and AntiPDL-1 Antibody
    Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF 156-B7
  • Name: Anonymous
    Verified Customer | Posted 05/23/2017
    Using Octet, we tested the ability of B7-H1/PD-L1 to bind recombinant PD-1 (R&D 8986-PD-100) and found them to specifically bind to each other.
    Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF 156-B7

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FAQs for Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF

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  • Q: What is the pI (Isoelectric Point) of Recombinant Human PD-L1/B7-H1 Fc Chimera Protein (Catalog # 156-B7)?

    A: The calculated pI for Recombinant Human PD-L1/B7-H1 Fc Chimera Protein (Catalog # 156-B7) is 7.1.

  • Q: Has Recombinant Human PD-L1/B7-H1 Fc Chimera Protein (Catalog # 156-B7), been evaluated on primary cells?

    A: Bioactivity of Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF (Catalog # 156-B7) is evaluated by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocyte primary cells. The ED50 for this effect is 0.075-0.75 μg/mL. We recommend reviewing our list of publications under the Citations tab on the product-specific web page to find reported use of our products in similar experimental layouts.

  • Q: What are the differences between Recombinant Human PD-L1/B7-H1 His-tag Protein, CF (Catalog # 9049-B7) and Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF (Catalog # 156-B7)?

    A: Recombinant Human PD-L1/B7-H1 Protein, CF (Catalog # 9049-B7) consists of (Phe19-Thr239) from Accession # Q9NZQ7. It has a His tag but no Fc chimera. Activity for 9049-B7 is measured in a binding assay with Recombinant Human PD‑1 Fc Chimera (Catalog # 1086-PD). Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF (Catalog 156-B7) consists of the same region (Phe19-Thr239), but instead of a His tag, it has a linker region and an Fc chimera. Activity for 156-B7 is assessed in a cell-based assay measuring IL-2 production by human T lymphocytes.

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