Skip to main content

Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF

Graph showing bioactivity of Human PD-L1 protein (B7-H1 protein)
Catalog #
Availability
Size / Price
Qty
Loading...
156-B7-100
156-B7-01M

Order Information

Bio-Techne Corporation
tel: (800) 343-7475 (free phone)
tel: (612) 379-2956

Shipping Information

$35 to United States

Products in stock will be shipped in 1-3 business days unless specified.

View Distributors
R&D Systems Guarantee

Product Performance Pledge

We offer a 100% guarantee on all our products.

Learn More »

Key Product Details

Source: NS0
Accession #: Q9NZQ7
Structure / Form: Disulfide-linked homodimer
Applications: Bioactivity

Complete Your Research

Complete Your Experiment

Citations (15)

Showing  1 - 10 of 15 citations Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF include:

There are no citations that match your criteria.

Showing  1 - 10 of 15 citations Showing All

Customer Reviews (21)

4.9 out of 5
21 customer ratings
5 stars
90%
4 stars
5%
3 stars
5%
2 stars
0%
1 star
0%

Customer Images


Showing  1 - 5 of 21 reviews Showing All
Filter By:
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Reason for Rating: Tested binding of PDL1-Fc w/ PD-1 using Octet. KD ~1.5uM
    Verified Customer | Posted 06/19/2020
    Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF Binding assay/Protein-protein interaction 156-B7
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/15/2019
    HEK cells expressing mCherry alone (LEFT) or hPD-1 mCherry (RIGHT) were incubated with 0.25 ug of hPD-L1 hIgG1. Binding was monitored by flow cytometry using an anti-human 488 secondary antibody.
    Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF Binding assay/Protein-protein interaction 156-B7
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Stem/Immune cell maintenance or differentiation
    Verified Customer | Posted 12/06/2018
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Cell Adhesion
    Verified Customer | Posted 10/24/2018
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Enzymatic activity in vitro
    Verified Customer | Posted 10/12/2018
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: CellProlif
    Verified Customer | Posted 09/28/2018
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 08/26/2018
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 04/12/2018
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 03/30/2018
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 03/15/2018
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: SDS-PAGE Control
    Verified Customer | Posted 03/11/2018
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Reason for Rating: Binding observed.
    Verified Customer | Posted 02/07/2018
    Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF Binding assay/Protein-protein interaction 156-B7
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 12/18/2017
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 12/06/2017
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 12/01/2017
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/27/2017
    Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF Binding assay/Protein-protein interaction 156-B7
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/18/2017
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 07/20/2017
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Cell Adhesion
    Verified Customer | Posted 07/20/2017
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: SPR assay for Kinetics between PDL-1 and AntiPDL-1 Antibody
    Verified Customer | Posted 07/14/2017
    SPR experiment
    Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF SPR assay for Kinetics between PDL-1 and AntiPDL-1 Antibody  156-B7
  • Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF
    Name: Anonymous
    Verified Customer | Posted 05/23/2017
    Using Octet, we tested the ability of B7-H1/PD-L1 to bind recombinant PD-1 (R&D 8986-PD-100) and found them to specifically bind to each other.
    Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF Serum 156-B7

There are no reviews that match your criteria.

Showing  1 - 5 of 21 reviews Showing All

FAQs

  • The vial is supposed to contain lyophilized protein but it appears to be empty. Is there anything in it?

    Pellets can be dislodged during shipping and become disbursed on the vial wall and in the cap. Centrifuge or tap the vial on the benchtop to return this material to the vial bottom. If this does not reveal a pellet, closely inspect the cone of the vial. Some pellets appear as only a tiny amount of material or as a transparent film due to the original buffer formulation. This is a normal appearance for many proteins. For example, if the product is originally lyophilized from a solvent such as acetonitrile or ethanol, and supplied carrier-free, you may not be able to detect the pellet with the naked eye. This does not mean the vial is empty. Reconstitute the vial as directed. After reconstitution, protein concentration can be tested with a spectrophotometer.

  • What is the recommended method for reconstitution of a lyophilized protein or antibody?

    Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand.  Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4oC overnight. Contact Technical Service if product does not go into solution.   

  • Are R&D Systems recombinant proteins and antibodies sterile?

    Although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

  • What is the pI (Isoelectric Point) of Recombinant Human PD-L1/B7-H1 Fc Chimera Protein (Catalog # 156-B7)?

    The calculated pI for Recombinant Human PD-L1/B7-H1 Fc Chimera Protein (Catalog # 156-B7) is 7.1.

  • Do you have a human IgG1 Fc region that would be a suitable negative control for the linker sequence and Fc region in Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF (Catalog # 156-B7)?

    Yes, we offer Recombinant Human IgG1 Fc Protein, CF (Catalog # 110-HG), which contains the human IgG Fc region and linker sequence.

  • Has Recombinant Human PD-L1/B7-H1 Fc Chimera Protein (Catalog # 156-B7), been evaluated on primary cells?

    Bioactivity of Recombinant Human PD-L1/B7-H1 Fc Chimera Protein, CF (Catalog # 156-B7) is evaluated by its ability to inhibit anti-CD3 antibody induced IL-2 secretion in human T lymphocyte primary cells. The ED50 for this effect is 0.075-0.75 μg/mL. We recommend reviewing our list of publications under the Citations tab on the product-specific web page to find reported use of our products in similar experimental layouts.

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.