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Recombinant Human PD-1 Fc Chimera Protein, CF

Graph showing bioactivity of Human PD-1 protein
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1086-PD-01M
1086-PD-050

Key Product Details

Source: NS0
Accession #: Q15116.3
Structure / Form: Disulfide-linked homodimer
Applications: Binding Activity

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Citations for Recombinant Human PD-1 Fc Chimera Protein, CF (23)

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Customer Reviews (9)

4.7 out of 5
9 customer ratings
5 stars
78%
4 stars
11%
3 stars
11%
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1 star
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  • Recombinant Human PD-1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: In vitro bioactivity in cell culture
    Verified Customer | Posted 06/26/2020
  • Recombinant Human PD-1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 01/15/2020
  • Recombinant Human PD-1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 01/12/2020
  • Recombinant Human PD-1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/22/2019
    Cells expressing a negative control (left) or hPD-L1 mCherry (right) were treated with 0.25 ug of hPD-1 hIgG1. Cells were analyzed by flow cytometry with binding detected using an anti-human 488 secondary antibody.
    Recombinant Human PD-1 Fc Chimera Protein, CF Binding assay/Protein-protein interaction 1086-PD
  • Recombinant Human PD-1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 08/20/2018
  • Recombinant Human PD-1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 12/08/2017
  • Recombinant Human PD-1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 11/03/2017
  • Recombinant Human PD-1 Fc Chimera Protein, CF
    Name: Anonymous
    Verified Customer | Posted 04/21/2017
  • Recombinant Human PD-1 Fc Chimera Protein, CF
    Name: Anonymous
    Application: Binding assay/Protein-protein interaction
    Verified Customer | Posted 07/08/2016
    Sorry that I just rate the catalog based on my alike recombinant PD1 peptide. I have one question for the peptide. Owing the Ig like peptide with Fc fragment. So when we detect it binding to PDL1 on cell assay, it is difficult to get definite conclusion if it is PDL1 binding or others.My protocol is as below:-Cells with PDL1 high level was treated by PD1 like peptide,-4degree 1hr-collected and lysis-WB by anti-IgG to Fc recognization.Result: weak signal. you don't know if it is non-specific binding
    Bio-Techne Response
    Thank you for reviewing our product. We are sorry to hear that this antibody did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.

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FAQs

  • The vial is supposed to contain lyophilized protein but it appears to be empty. Is there anything in it?

    Pellets can be dislodged during shipping and become disbursed on the vial wall and in the cap. Centrifuge or tap the vial on the benchtop to return this material to the vial bottom. If this does not reveal a pellet, closely inspect the cone of the vial. Some pellets appear as only a tiny amount of material or as a transparent film due to the original buffer formulation. This is a normal appearance for many proteins. For example, if the product is originally lyophilized from a solvent such as acetonitrile or ethanol, and supplied carrier-free, you may not be able to detect the pellet with the naked eye. This does not mean the vial is empty. Reconstitute the vial as directed. After reconstitution, protein concentration can be tested with a spectrophotometer.

  • What is the recommended method for reconstitution of a lyophilized protein or antibody?

    Unless more specific directions are on the Certificate of Analysis provided with the product, we suggest the following procedure to ensure optimal recovery: 1. Allow the vial and reconstitution buffer to equilibrate to room temperature. 2. Briefly centrifuge the vial to ensure that all lyophiliate is collected at the bottom of the vial. 3. Add the amount of buffer required to achieve the concentration recommended on the product insert. 4. Allow the vial to reconstitute for 15-30 minutes at room temperature with gentle agitation, like on a rocker platform or rotating by hand.  Avoid vigorous shaking that can cause foaming and protein denaturation. 5. Aliquot into volumes greater than 20 μL and store as indicated on the product insert. If the vial exhibits flakes or particulates, mix the product for a couple of hours at room temperature and then at 4oC overnight. Contact Technical Service if product does not go into solution.   

  • Are R&D Systems recombinant proteins and antibodies sterile?

    Although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Product Documents

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.