Human/Mouse/Rat/Hamster ACE-2 Antibody

Name: Human/Mouse/Rat/Hamster ACE-2 Antibody
Brand: rnd
SKU: AF933
Rating: 5 (2 reviews)

Catalog # AF933

Citations (44)

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Key Product Details

Species Reactivity:	Human, Mouse, Rat, Hamster
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human ACE-2 Gln18-Ser740 Accession # Q9BYF1
Applications:	Block/Neutralize, Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human ACE-2 in direct ELISAs. Detects human, mouse, and rat ACE-2 in Western blots. Detects Hamster ACE-2 in immunohistochemistry. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human ACE is observed.

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Applications

	Recommended Concentration	Sample
Western Blot	1 µg/mL	See below
Simple Western	10 µg/mL	See below
Flow Cytometry	0.25 µg/10⁶ cells	See below
Immunohistochemistry	3-15 µg/mL	See below
Immunoprecipitation	25 µg/mL	Conditioned cell culture medium spiked with Recombinant Human ACE‑2 (Catalog # 933-ZN), see our available Western blot detection antibodies
Blockade of Receptor-ligand Interaction	Hoffman, M. et al. (2020) Cell. DOI:10.1016/j.cell.2020.02.052. This application was not tested by R&D Systems.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Human/Mouse/Rat/Hamster ACE-2 Antibody Scientific Data Examples

	Western Blot Detection of Human, Mouse, and Rat ACE‑2 by Western Blot. Western blot shows lysates of human kidney tissue, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for ACE-2 at approximately 100 and 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
	Flow Cytometry Detection of ACE-2 in HEK293 Human Cell Line Transfected with Human ACE-2 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with (A) human ACE-2 or (B) irrelevant protein, and eGFP was stained with Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Affinity Purified Polyclonal Antibody (Catalog # AF933) followed by Allophycocyanin-conjugated Anti-Goat IgG Secondary Antibody (F0108). Quadrant markers were set based on Goat IgG control antibody (AB-108-C, data not shown). Staining was performed using our Staining Membrane-associated Proteins protocol.
	Immunohistochemistry ACE‑2 in Human Kidney. ACE-2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Immunohistochemistry ACE‑2 in Human Kidney. ACE-2 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Immunohistochemistry ACE‑2 in Hamster Lung. ACE‑2 was detected in immersion fixed paraffin-embedded sections of hamster lung using Goat Anti-Human/Mouse/Rat/Hamster ACE‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to respiratory bronchioles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
	Simple Western Detection of Human ACE‑2 by Simple Western^TM. Simple Western lane view shows lysates of human kidney tissue, loaded at 0.2 mg/mL. A specific band was detected for ACE-2 at approximately 155 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat/Hamster ACE-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF933) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: ACE-2

ACE-2, also called ACEH (ACE homolog), is an integral membrane protein and a zinc metalloprotease of the ACE family that also includes somatic and germinal ACE (1). Human ACE-2 has about 40% amino acid identity to the N- and C-terminal domains of human somatic ACE. The predicted human ACE-2 protein sequence consists of 805 amino acids, including a N-terminal signal peptide, a single catalytic domain, a C-terminal membrane anchor, and a short cytoplasmic tail. ACE-2 cleaves angiotensins I and II as a carboxypeptidase. ACE-2 mRNA is found at high levels in testis, kidney, and heart and at moderate levels in colon, small intestine, and ovary. Classical ACE inhibitors such as captopril and lisinopril do not inhibit ACE-2 activity. Novel peptide inhibitors of ACE-2 do not inhibit ACE activity (2). Genetic data from Drosophila, mice and rats show that ACE-2 is an essential regulator of heart function in vivo (3).

ACE2 has been shown to be a functional receptor of the human coronaviruses SARS-CoV and SARS-CoV-2 (4, 5). This Human anti-ACE2 antibody (catalog # AF933) was used to block the variant SARS-CoV-2 and ACE2 interaction to elucidate viral transmission and potential therapeutic strategies. (5)

Item	Value
References	Tipnis, S.R. et al. (2000) J. Biol. Chem. 275:33238. Crackower, M.A. et al. (2002) Nature 417:822. Huang, L. et al. (2003) J. Biol. Chem. 278:15532. Li, W. et al. (2003) Nature 426:450. Hoffmann, M. et al. (2020) Cell. DOI: 10.1016/j.cell.2020.02.052.
Long Name	Angiotensin I Converting Enzyme 2
Entrez Gene IDs	59272 (Human); 70008 (Mouse); 302668 (Rat); 100144303 (Porcine); 480847 (Canine); 418623 (Chicken); 102130864 (Cynomolgus Monkey); 554349 (Feline); 101673097 (Ferret); 101823817 (Hamster); 108390919 (Malayan Pangolin)
Alternate Names	ACE-2, ACE-related carboxypeptidase, ACE2, ACEH, ACEHangiotensin I converting enzyme 2, Angiotensin-converting enzyme homolog, DKFZp434A014, EC 3.4.17, EC 3.4.17.23, Metalloprotease MPROT15, angiotensin I converting enzyme (peptidyl-dipeptidase A) 2, angiotensin-converting enzyme 2

Citations (44)

Showing 1 - 10 of 44 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human/Mouse/Rat/Hamster ACE-2 Antibody include:

Species: Rat
Sample Types: Tissue Homogenates

EE Khoury et al. (2021), Pulmonary, cardiac and renal distribution of ACE2, furin, TMPRSS2 and ADAM17 in rats with heart failure: Potential implication for COVID-19 disease Journal of Cellular and Molecular Medicine, 0(0):.
PMID: 33660945
Species: Human
Sample Types: Whole Tissue
Applications: IHC

SP Sajuthi et al. (2020), Type 2 and interferon inflammation regulate SARS-CoV-2 entry factor expression in the airway epithelium Nat Commun, 11(1):5139.
PMID: 33046696
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

R Rathnasing et al. (2020), Comparison of Transgenic and Adenovirus hACE2 Mouse Models for SARS-CoV-2 Infection bioRxiv, 0(0):.
PMID: 32676603
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

F Duan et al. (2020), Modeling COVID-19 with Human Pluripotent Stem Cell-Derived Cells Reveals Synergistic Effects of Anti-inflammatory Macrophages with ACE2 Inhibition Against SARS-CoV-2 Res Sq, 0(0):.
PMID: 32839764
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

I Busnadiego et al. (2020), Antiviral Activity of Type I, II, and III Interferons Counterbalances ACE2 Inducibility and Restricts SARS-CoV-2 MBio, 11(5):.
PMID: 32913009
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

M Baratchian et al. (2020), Sex, androgens and regulation of pulmonary AR, TMPRSS2 and ACE2 bioRxiv, 0(0):.
PMID: 33083800
Species: Human
Sample Types: Whole Cells
Applications: ICC

J Prévost et al. (2020), Cross-sectional evaluation of humoral responses against SARS-CoV-2 Spike bioRxiv : the preprint server for biology, 0(0):.
PMID: 32577637
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

L Pellegrini et al. (2020), SARS-CoV-2 Infects the Brain Choroid Plexus and Disrupts the Blood-CSF Barrier in Human Brain Organoids Cell Stem Cell, 0(0):.
PMID: 33113348
Species: Primate
Sample Types: Whole Cells
Applications: ICC

X Xie et al. (2020), A nanoluciferase SARS-CoV-2 for rapid neutralization testing and screening of anti-infective drugs for COVID-19 Nat Commun, 11(1):5214.
PMID: 33060595
Species: Human
Sample Types: Whole Tissue
Applications: IHC

IT Lee et al. (2020), ACE2 localizes to the respiratory cilia and is not increased by ACE inhibitors or ARBs Nat Commun, 11(1):5453.
PMID: 33116139
Species: Human
Sample Types: Whole Cells
Applications: ICC

W Rut et al. (2020), SARS-CoV-2 Mpro inhibitors and activity-based probes for patient-sample imaging Nat Chem Biol, 0(0):.
PMID: 33093684
Species: Bat
Sample Types: Whole Cells
Applications: ICC

J Zhou et al. (2020), Infection of bat and human intestinal organoids by SARS-CoV-2 Nat. Med., 0(0):.
PMID: 32405028
Species: Human
Sample Types: Organoid
Applications: ICC

RM Samuel et al. (2020), Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men Cell Stem Cell, 27(6):876-889.e12.
PMID: 33232663
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

JK Osan et al. (2020), Goblet Cell Hyperplasia Increases SARS-CoV-2 Infection in COPD bioRxiv, 0(0):.
PMID: 33200131
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

J Huang et al. (2020), SARS-CoV-2 Infection of Pluripotent Stem Cell-derived Human Lung Alveolar Type 2 Cells Elicits a Rapid Epithelial-Intrinsic Inflammatory Response bioRxiv, 0(0):.
PMID: 32637964
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

R Tandon et al. (2020), Effective screening of SARS-CoV-2 neutralizing antibodies in patient serum using lentivirus particles pseudotyped with SARS-CoV-2 spike glycoprotein Sci Rep, 10(1):19076.
PMID: 33154514
Species: Human
Sample Types: Protein
Applications: Western Blot

L Casciola-R et al. (2020), IgM autoantibodies recognizing ACE2 are associated with severe COVID-19 medRxiv, 0(0):.
PMID: 33083808
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

J Huang et al. (2020), SARS-CoV-2 Infection of Pluripotent Stem Cell-Derived Human Lung Alveolar Type 2 Cells Elicits a Rapid Epithelial-Intrinsic Inflammatory Response Cell Stem Cell, 0(0):.
PMID: 32979316
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot, `

R Wang et al. (2020), Functional genomic screens identify human host factors for SARS-CoV-2 and common cold coronaviruses bioRxiv : the preprint server for biology, 0(0):.
PMID: 32995787
Species: Human
Sample Types: Organoid
Applications: Neutralization

E Song et al. (2020), Neuroinvasion of SARS-CoV-2 in human and mouse brain bioRxiv : the preprint server for biology, 0(0):.
PMID: 32935108
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

M Yang et al. (2020), Liraglutide Attenuates Non-Alcoholic Fatty Liver Disease in Mice by Regulating the Local Renin-Angiotensin System Front Pharmacol, 11(0):432.
PMID: 32322207
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

KHD Crawford et al. (2020), Protocol and Reagents for Pseudotyping Lentiviral Particles with SARS-CoV-2 Spike Protein for Neutralization Assays Viruses, 12(5):.
PMID: 32384820
Species: Primate - Cercopithecus aethiops (African Green Monkey)
Sample Types: Whole Cells
Applications: Neutralization

Hoffmann M et al. (2020), SARS-CoV-2 Cell Entry Depends on ACE2 and TMPRSS2 and Is Blocked by a Clinically Proven Protease Inhibitor. Cell, 181(0):1-10.
PMID: 32142651
Species: Rhesus Macaca (Macaque)
Sample Types: Whole Tissue

D Tian et al. (2019), Protective effects of the Francisella tularensis Î´pdpC mutant against its virulent parental strain SCHU P9 in Cynomolgus macaques Sci Rep, 9(1):9193.
PMID: 31235714
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

E Schrom et al. (2017), Translation of Angiotensin-Converting Enzyme 2 upon Liver- and Lung-Targeted Delivery of Optimized Chemically Modified mRNA Mol Ther Nucleic Acids, 7(0):350-365.
PMID: 28624211
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: Neutralization

Yu Y et al. (2016), Surface vimentin is critical for the cell entry of SARS-CoV. J Biomed Sci, 23(1):14.
PMID: 26801988
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: IHC

Aleksandra Milewska (2016), HTCC: Broad Range Inhibitor of Coronavirus Entry PLoS ONE, 11(6):e0156552.
PMID: 27249425
Species: Mouse
Sample Types: Cell Culture Supernates
Applications: Western Blot

Fengxia Xiao et al. (2016), Protein Kinase C-? Mediates Shedding of Angiotensin-Converting Enzyme 2 from Proximal Tubular Cells. Frontiers in Pharmacology, 0(0):1663-9812.
PMID: 27313531
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

Xiao F et al. (2014), Characterization of angiotensin-converting enzyme 2 ectodomain shedding from mouse proximal tubular cells. PLoS ONE, 9(1):e85958.
PMID: 24454948
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Heurich A et al. (2014), TMPRSS2 and ADAM17 cleave ACE2 differentially and only proteolysis by TMPRSS2 augments entry driven by the severe acute respiratory syndrome coronavirus spike protein. J Virol, 88(2):1293-307.
PMID: 24227843
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Eckerle I et al. (2013), In-vitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection. Virol J, 10(0):359.
PMID: 24364985
Species: Bat
Sample Types: Whole Cells
Applications: IHC

Ge et al. (2013), Isolation and characterization of a bat SARS-like coronavirus that uses the ACE2 receptor. Nature, 503(7477):535-8.
PMID: 24172901
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Bertram S et al. (2012), Influenza and SARS-coronavirus activating proteases TMPRSS2 and HAT are expressed at multiple sites in human respiratory and gastrointestinal tracts. PLoS ONE, 7(4):e35876.
PMID: 22558251
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Pohl M et al. (2010), Intrarenal renin angiotensin system revisited: role of megalin-dependent endocytosis along the proximal nephron. J. Biol. Chem., 285(53):41935-46.
PMID: 20966072
Species: Human
Sample Types: Plasma
Applications: Neutralization

Epelman S et al. (2008), Detection of soluble angiotensin-converting enzyme 2 in heart failure: insights into the endogenous counter-regulatory pathway of the renin-angiotensin-aldosterone system. J. Am. Coll. Cardiol., 52(9):750-4.
PMID: 18718423
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: Flow Cytometry

Lu Y et al. (2008), Lipid rafts are involved in SARS-CoV entry into Vero E6 cells. Biochem. Biophys. Res. Commun., 369(2):344-9.
PMID: 18279660
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Cell Lysates
Applications: Western Blot

Glende J et al. (2008), Importance of cholesterol-rich membrane microdomains in the interaction of the S protein of SARS-coronavirus with the cellular receptor angiotensin-converting enzyme 2. Virology, 381(2):215-21.
PMID: 18814896
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Ren W et al. (2007), Difference in receptor usage between severe acute respiratory syndrome (SARS) coronavirus and SARS-like coronavirus of bat origin. J. Virol., 82(4):1899-907.
PMID: 18077725
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Cell Lysates
Applications: Western Blot

Inoue Y et al. (2007), Clathrin-dependent entry of severe acute respiratory syndrome coronavirus into target cells expressing ACE2 with the cytoplasmic tail deleted. J. Virol., 81(16):8722-9.
PMID: 17522231
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: Neutralization

Rockx B et al. (2007), Synthetic reconstruction of zoonotic and early human severe acute respiratory syndrome coronavirus isolates that produce fatal disease in aged mice. J. Virol., 81(14):7410-23.
PMID: 17507479
Species: Primate - Chlorocebus pygerythrus (Vervet Monkey)
Sample Types: Whole Cells
Applications: ICC

de Lang A et al. (2006), Interferon-gamma and interleukin-4 downregulate expression of the SARS coronavirus receptor ACE2 in Vero E6 cells. Virology, 353(2):474-81.
PMID: 16860835
Species: Human
Sample Types: Whole Cells
Applications: ICC

Tseng CT et al. (2005), Apical entry and release of severe acute respiratory syndrome-associated coronavirus in polarized Calu-3 lung epithelial cells. J. Virol., 79(15):9470-9.
PMID: 16014910
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Hofmann H et al. (2004), Susceptibility to SARS coronavirus S protein-driven infection correlates with expression of angiotensin converting enzyme 2 and infection can be blocked by soluble receptor. Biochem. Biophys. Res. Commun., 319(4):1216-21.
PMID: 15194496
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Ou X et al. (0), Characterization of spike glycoprotein of SARS-CoV-2 on virus entry and its immune cross-reactivity with SARS-CoV. Nat Commu11(1):1620.
PMID: 32221306

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Human/Mouse/Rat/Hamster ACE-2 Antibody

Name: Anonymous

Application: ICC/IF

Sample Tested: Stimulated Human Bronchial Epithelial Cell Line (BEAS-2B)

Species: Human

Verified Customer | Posted 03/09/2021
Human ACE-2 Antibody

Name: Anonymous

Application: WB

Sample Tested: Blood mononuclear cells (PBMCs),Human CD34+ Cells

Species: Human

Verified Customer | Posted 08/12/2016

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Human/Mouse/Rat/Hamster ACE-2 Antibody

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Key Product Details

Complete Your Research

Complete Your Experiment

Applications

Human/Mouse/Rat/Hamster ACE-2 Antibody Scientific Data Examples

Western Blot

Flow Cytometry

Immunohistochemistry

Immunohistochemistry

Immunohistochemistry

Simple Western

Formulation, Preparation and Storage

Reconstitution Calculator

Background: ACE-2

Citations (44)

Customer Reviews (2)

Customer Images

Human/Mouse/Rat/Hamster ACE-2 Antibody

Human ACE-2 Antibody

Product Datasheets

Product Datasheets

COA

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