Human IL-18 R alpha /IL-1 R5 Antibody

Name: Human IL-18 R alpha /IL-1 R5 Antibody
Brand: R&D Systems
SKU: MAB840

R&D Systems, Inc. a Bio-Techne Brand

Catalog # MAB840

Citations (9)

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5 Glu20-Arg329 Accession # Q13478
Applications:	Block/Neutralize, CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry
Label:	Unconjugated
Source:	Monoclonal Mouse IgG₁ Clone # 70625
Specificity:	Detects Recombinant Human IL-18 R alpha /IL-1 R5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL-1 R3, rhIL-1 R4, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, rhIL-1 R9, rhSIGIRR or recombinant mouse IL-18 R alpha is observed.

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Applications for Human IL-18 R alpha /IL-1 R5 Antibody

	Recommended Concentration	Sample
Flow Cytometry	2.5 µg/10⁶ cells	Human peripheral blood mononuclear cells treated with PHA and Recombinant Human IL‑2 (Catalog # 202-IL)
Immunohistochemistry	8-25 µg/mL	Immersion fixed paraffin-embedded sections of human skin
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	8-25 µg/mL	See below

Item	Value
Neutralization	Measured by its ability to neutralize IL‑18/IL‑1F4-induced IFN‑ gamma secretion in KG-1 human myeloid leukemia cells. The Neutralization Dose (ND₅₀) is typically 0.03-0.1 µg/mL in the presence of 40 ng/mL Recombinant Human IL‑18/IL‑1F4 and 20 ng/mL Recombinant Human TNF-alpha.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-18 R alpha /IL-1 R5 Antibody

	Neutralization IFN‑ gamma Secretion Induced by IL‑18/IL‑1F4 and Neutralization by IL‑18 R alpha /IL‑1 R5 Antibody. In the presence of Recombinant Human TNF-alpha (20 ng/mL, Catalog # 210-TA), Recombinant Human IL-18/IL-1F4 (Catalog # B003-5) stimulates IFN-gamma secretion in KG-1 human myeloid leukemia cells in a dose-dependent manner (orange line), as measured by the Human IFN-gamma Quantikine ELISA Kit (Catalog # DIF50C). Under these conditions, IFN-gamma secretion elicited by Recombinant Human IL-18/IL-1F4 (40 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-18 Ra/ IL-1 R5 Monoclonal Antibody (Catalog # MAB840). The ND₅₀ is typically 0.03-0.1 µg/mL.
	Immunocytochemistry IL‑18 R alpha /IL‑1 R5 in Human PBMCs. IL-18 Ra/IL-1 R5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Mouse Anti-Human IL-18 Ra/IL-1 R5 Mono-clonal Antibody (Catalog # MAB840) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counter-stained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
	Immunohistochemistry Detection of IL‑18 R alpha /IL‑1 R5 in Human Skin. IL‑18 R alpha /IL‑1 R5 was detected in immersion fixed paraffin-embedded sections of Human Skin using Mouse Anti-Human IL‑18 R alpha /IL‑1 R5 Monoclonal Antibody (Catalog # MAB840) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm and cell surface of keratinocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.5 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IL-18 R alpha/IL-1 R5

Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also termed IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also termed IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

Item	Value
References	Parnet, P. et al. (1996) J. Biol. Chem. 271:3967. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Long Name	Interleukin 18 Receptor alpha
Entrez Gene IDs	8809 (Human); 16182 (Mouse)
Alternate Names	CD218a, IL-1 R5, IL-1 Rrp, IL-18 R alpha, IL-18 R1, IL-18Ra, IL18R alpha, IL18R1

Citations for Human IL-18 R alpha /IL-1 R5 Antibody (9)

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-18 R alpha /IL-1 R5 Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

(2016), Interleukin-37 expression and its potential role in oral leukoplakia and oral squamous cell carcinoma Sci Rep, 6(0):26757.
PMID: 27225603
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr

Wang J et al. (2015), Interleukin 18 function in atherosclerosis is mediated by the interleukin 18 receptor and the Na-Cl co-transporter. Nat Med, 21(7):820-6.
PMID: 26099046
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Li W et al. (2013), Regulation of development of CD56 bright CD11c + NK-like cells with helper function by IL-18. PLoS ONE, 8(12):e82586.
PMID: 24376549
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Blom L et al. (2012), IL-1 Family Members IL-18 and IL-33 Upregulate the Inflammatory Potential of Differentiated Human Th1 and Th2 Cultures. J Immunol, 189(9):4331-7.
PMID: 23028054
Species: Human
Sample Types: Serum
Applications: Western Blot

Takei S et al. (2011), Soluble interleukin-18 receptor complex is a novel biomarker in rheumatoid arthritis. Arthritis Res. Ther., 13(2):R52.
PMID: 21435242
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Tucci M et al. (2008), Glomerular accumulation of plasmacytoid dendritic cells in active lupus nephritis: role of interleukin-18. Arthritis Rheum., 58(1):251-62.
PMID: 18163476
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Rao DA et al. (2007), IL-1alpha and IL-1beta are endogenous mediators linking cell injury to the adaptive alloimmune response. J. Immunol., 179(10):6536-46.
PMID: 17982042
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Zola H et al. (2007), CD molecules 2006--human cell differentiation molecules. J. Immunol. Methods, 319(1):1-5.
PMID: 17174972
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Ebert EC et al. (2006), Human intestinal intraepithelial lymphocytes keep TNF alpha levels low by cell uptake and feedback inhibition of transcription. Cell. Immunol., 241(1):7-13.
PMID: 16914126

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Human IL-18 R alpha /IL-1 R5 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Applications for Human IL-18 R alpha /IL-1 R5 Antibody

Scientific Data Examples for Human IL-18 R alpha /IL-1 R5 Antibody

Neutralization

Immunocytochemistry

Immunohistochemistry

Formulation, Preparation and Storage

Reconstitution Calculator

Background: IL-18 R alpha/IL-1 R5

Citations for Human IL-18 R alpha /IL-1 R5 Antibody (9)

Product Documents for Human IL-18 R alpha /IL-1 R5 Antibody

Product Datasheets

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