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Human IL-18 R alpha /IL-1 R5 APC-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

Detection of IL‑18 Ra/IL‑1 R5 antibody in Human PBMCs antibody by Flow Cytometry.
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FAB840A-025
FAB840A-100
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Key Product Details

Species Reactivity: Human
Immunogen: Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5
Glu20-Arg329
Accession # Q13478
Applications: Flow Cytometry
Label: Allophycocyanin (Excitation=620-650 nm, Emission=660-670 nm)
Source: Monoclonal Mouse IgG1 Clone # 70625
Specificity:

Detects human IL-18 R alpha /IL-1 R5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL‑1 R3, rhIL-1 R4, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, rhIL-1 R9, rhSIGIRR or recombinant mouse IL-18 R alpha is observed.

Citations for Human IL-18 R alpha /IL-1 R5 APC-conjugated Antibody (3)

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Customer Reviews for Human IL-18 R alpha /IL-1 R5 APC-conjugated Antibody (1)

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  • Human IL-18 R alpha /IL-1 R5 APC-conjugated Antibody
    Name: Anonymous
    Application: Flow
    Sample Tested: Peripheral blood monocytes,Peripheral blood T cells
    Species: Human
    Verified Customer | Posted 05/31/2016
    Detection of IL18R alpha /IL-1R5 expression in lymphocytes and monocytes by flow cytometry: Peripheral blood mononuclear cells (PBMCs) were isolated by Ficoll gradient method from freshly drawn blood and stained for IL18Ralpha using the R&D mouse Anti-human IL-18alpha/IL-1R5-APC antibody (cat# FAB840A). As suggested by the manufacturer, 10ul of antibody was used per 10e6 cells. After gating on CD3+ lymphocytes (Fig.1a) and CD14+ monocytes (Fig.1b), IL-18Ralpha expression on T lymphocytes and monocytes were analyzed. Panel 1 shows Fluorescence Minus One (FMO) which served as negative control; panel 2 shows IL18alpha+ cells; panel 3 shows overlay of panel 1 and panel 2.
    Human IL-18 R alpha /IL-1 R5 APC-conjugated Antibody Flow Cytometry FAB840A

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Product Documents for Human IL-18 R alpha /IL-1 R5 APC-conjugated Antibody

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