Human IL-18 R alpha /IL-1 R5 PE-conjugated Antibody

Name: Human IL-18 R alpha /IL-1 R5 PE-conjugated Antibody
Brand: R&D Systems
SKU: FAB840P

R&D Systems, Inc. a Bio-Techne Brand

Catalog # FAB840P

Citations (6)

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Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human IL‑18 R alpha /IL-1 R5 Glu20-Arg329 Accession # Q13478
Applications:	Flow Cytometry
Label:	Phycoerythrin (Excitation=488 nm, Emission=565-605 nm)
Source:	Monoclonal Mouse IgG₁ Clone # 70625
Specificity:	Detects human IL-18 R alpha /IL-1 R5 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-1 RI, rhIL-1 RII, rhIL‑1 R3, rhIL-1 R4, rhIL-1 R6, rhIL-1 R7, rhIL-1 R8, rhIL-1 R9, rhSIGIRR or recombinant mouse IL-18 R alpha is observed.

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Applications for Human IL-18 R alpha /IL-1 R5 PE-conjugated Antibody

	Recommended Concentration	Sample
Flow Cytometry	10 µL/10⁶ cells	See below

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human IL-18 R alpha /IL-1 R5 PE-conjugated Antibody

	Flow Cytometry Detection of IL‑18 R alpha /IL‑1 R5 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) treated with 10 ng/mL Recombinant Human IL-2 (Catalog # 202-IL) and 5 µg/mL PHA for 48 hours were stained with Mouse Anti-Human IL-18 Ra/IL-1 R5 PE-conjugated Monoclonal Antibody (Catalog # FAB840P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Formulation, Preparation and Storage

Purification	Protein A or G purified from hybridoma culture supernatant
Formulation	Supplied in a saline solution containing BSA and Sodium Azide.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage	Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.

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Background: IL-18 R alpha/IL-1 R5

Interleukin 18 (IL-18) is a member of the IL-1 family of cytokines and shares numerous immunoregulatory functions with IL-12. The functional IL-18 receptor complex is composed of two subunits designated IL-18 R alpha (also known as IL-1 R5 and IL-1 Rrp) and IL-18 R beta (also known as IL-1 R7 and AcPL). Both IL-18 R alpha and IL-18 R beta belong to the IL-1 receptor superfamily. Although IL-18 R alpha by itself binds IL-18 with low affinity and IL-18 R beta does not bind IL-18 in vitro, co-expression of IL‑18 R alpha and IL‑18 R beta is required for high affinity binding and IL-18 responsiveness. Human IL-18 R cDNA encodes a 541 amino acid (aa) precursor type I membrane protein with a hydrophobic signal, an extracellular domain comprised of three immunoglobulin-like domains, a transmembrane domain and a cytoplasmic region of approximately 200 aa. Human and mouse IL-18 R share 65% amino acid sequence homology. IL-18 R is widely expressed in numerous tissues including spleen, thymus, leukocyte, liver, lung, heart, small and large intestine, prostate and placenta.

Item	Value
References	Parnet, P. et al. (1996) J. Biol. Chem. 271:3967. Torigoe, K. et al. (1997) J. Biol. Chem. 272:25737. Born, T.L. et al. (1998) J. Biol. Chem. 273:29445.
Long Name	Interleukin 18 Receptor alpha
Entrez Gene IDs	8809 (Human); 16182 (Mouse)
Alternate Names	CD218a, IL-1 R5, IL-1 Rrp, IL-18 R alpha, IL-18 R1, IL-18Ra, IL18R alpha, IL18R1

Citations for Human IL-18 R alpha /IL-1 R5 PE-conjugated Antibody (6)

Showing 1 - 6 of 6 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human IL-18 R alpha /IL-1 R5 PE-conjugated Antibody include:

Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Sundstrom P et al. (2015), Human Mucosa-Associated Invariant T Cells Accumulate in Colon Adenocarcinomas but Produce Reduced Amounts of IFN-gamma. J Immunol, 195(7):3472-81.
PMID: 26297765
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Uzan B et al. (2014), Interleukin-18 produced by bone marrow-derived stromal cells supports T-cell acute leukaemia progression. EMBO Mol Med, 6(6):821-34.
PMID: 24778454
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

(2013), Increased expression of Interleukin-18 receptor in blood cells of subjects with Mild Cognitive Impairment and Alzheimer's disease. Cytokine, 61(2):360-3.
PMID: 23201485
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Dusseaux M et al. (2011), Human MAIT cells are xenobiotic-resistant, tissue-targeted, CD161hi IL-17-secreting T cells. Blood, 117(4):1250-9.
PMID: 21084709
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

De Jager W et al. (2009), Defective phosphorylation of interleukin-18 receptor beta causes impaired natural killer cell function in systemic-onset juvenile idiopathic arthritis. Arthritis Rheum., 60(9):2782-93.
PMID: 19714583
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

Wu C et al. (2003), IL-18 receptor beta-induced changes in the presentation of IL-18 binding sites affect ligand binding and signal transduction. J. Immunol., 170(11):5571-7.
PMID: 12759435

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Showing 1 - 6 of 6 citations Showing All

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Human IL-18 R alpha /IL-1 R5 PE-conjugated Antibody

R&D Systems, Inc. a Bio-Techne Brand

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Applications for Human IL-18 R alpha /IL-1 R5 PE-conjugated Antibody

Scientific Data Examples for Human IL-18 R alpha /IL-1 R5 PE-conjugated Antibody

Flow Cytometry