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Human IFN-gamma Quantikine ELISA Kit

Human IFN-gamma  Standard Curve ELISA
(2)
Human IFN-gamma  Linearity Curve Quantikine ELISA
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DIF50C
SIF50C
PDIF50C

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Key Product Details

Assay Length: 4.5 hours
Sample Type & Volume Required Per Well: Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL)
Sample Types: Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma
Sensitivity: 5.69 pg/mL
Assay Range: 15.6 - 1,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma)
Human IFN-gamma Quantikine ELISA Kit Summary:
The Quantikine Human IFN-gamma Immunoassay is a 4.5 hour solid phase ELISA designed to measure human IFN-gamma levels in cell culture supernates, serum, and plasma. It contains HEK293-expressed recombinant human IFN-gamma and antibodies raised against the recombinant factor. Results obtained for naturally occurring human IFN-gamma samples showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IFN-gamma.

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Citations for Human IFN-gamma Quantikine ELISA Kit (180)

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Customer Reviews (8)

4.9 out of 5
8 customer ratings
5 stars
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4 stars
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  • Human IFN-gamma Quantikine ELISA Kit
    Name: Lu Bai
    Sample Tested: Cell culture supernatant,human PBMC
    Verified Customer | Posted 12/22/2021
  • Human IFN-gamma Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: human primary NK cells,Peripheral blood mononuclear cells (PBMCs)
    Verified Customer | Posted 09/15/2021
    PBMCs from healthy donors or isolated primary human NK cells were stimulated and tested for IFNg secretion. Following protocol, it was easy to obtain clean and consistent results between indpendent experiments.
    Human IFN-gamma Quantikine ELISA Kit DIF50C
  • Human IFN-gamma Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: HepG2 human hepatocellular carcinoma cell line,MDA-MB-231 human breast cancer cell line
    Verified Customer | Posted 03/11/2021
    Easy to use, protocol detailed and easy to follow. Reproducible standard curve.
    Human IFN-gamma Quantikine ELISA Kit DIF50C
  • Human IFN-gamma Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Cancer Cells,T cells
    Verified Customer | Posted 02/24/2021
    Human IFN-gamma Quantikine ELISA Kit DIF50C
  • Human IFN-gamma Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: HepG2 human hepatocellular carcinoma cell line,MDA-MB-231 human breast cancer cell line
    Verified Customer | Posted 01/22/2021
    The assay was easy to perform according to the supplied insctructions. We were satisfied with the standard curve, for the samples the right dilution still needs to be figured out.
  • Human IFN-gamma Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 11/13/2020
    Standards in duplicate in all the wells in 1 and 2.R&D QC Low in well 3AR&D QC Med in well 12GR&D QC High in well 12HPipetting error or washing error didnt develop the best standard curve however the rest worked great.
    Human IFN-gamma Quantikine ELISA Kit DIF50C
  • Human IFN-gamma Quantikine ELISA Kit
    Name: Lindsie Morin
    Sample Tested: Serum
    Verified Customer | Posted 11/12/2020
    Great protocol that was easy to follow. The curve developed very well with expected results.
    Human IFN-gamma Quantikine ELISA Kit DIF50C
  • Human IFN-gamma Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: EDTA Plasma
    Verified Customer | Posted 11/12/2020
    Excellent background detail that was relevant for understanding the assay, and easy to follow protocol.
    Human IFN-gamma Quantikine ELISA Kit DIF50C

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