Human IGF-I/IGF-1 Antibody Best Seller

Name: Human IGF-I/IGF-1 Antibody
Brand: R&D Systems
SKU: MAB291
Rating: 5 (2 reviews)

Catalog # MAB291 | R&D Systems, Inc. a Bio-Techne Brand

(2)

Citations (5)

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Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

ELISA Capture (Matched Antibody Pair), Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 56408

Product Summary for Human IGF-I/IGF-1 Antibody

Immunogen

E. coli-derived recombinant human IGF-I/IGF-1
Gly49-Ala118
Accession # P01343

Specificity

Detects human IGF-I/IGF-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) IGF-I/IGF-1, rmIGF‑II/IGF-2, or recombinant human IGF‑II/IGF-2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human IGF-I/IGF-1 Antibody

Detection of Recombinant Human IGF-I/IGF-1 by Western Blot.

Western blot shows 25 ng of Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1), Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG), Recombinant Rat IGF-I/IGF-1 (Catalog # 4326-RG), and Recombinant Human IGF-II (Catalog # 292-G2). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGF-I/IGF-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human IGF-I/IGF-1 Antibody

Application

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1)

Human IGF-I/IGF-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)

Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:

Detection Reagent: Human IGF-I/IGF-1 Biotinylated Antibody (Catalog # BAF291)
Standard: Recombinant Human IGF-I/IGF-1 Protein, CF (Catalog # 291-G1)

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB291 in the following applications:

ELISA (1 Review)
Immunohistochemistry (1 Review)

Published Applications

Read 5 publications using MAB291 in the following applications:

ELISA Development (1 Publication)
ELISA Development (Capture) (1 Publication)
ELISA Microarray Development (1 Publication)
Western Blot (2 Publications)

Formulation, Preparation and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I/IGF-1

IGF-I/IGF-1 belongs to the family of insulin-like growth factors and circulates in complex with IGF binding proteins. It is a potent mitogenic growth factor that binds the heteromeric type I and type II IGF receptors. Essentially, all of the biological activities of IGF-I/IGF-1 are mediated by IGF-I R.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Entrez Gene IDs

3479 (Human); 16000 (Mouse); 24482 (Rat)

Gene Symbol

IGF1

UniProt

P01343

Product Documents for Human IGF-I/IGF-1 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human IGF-I/IGF-1 Antibody

For research use only

Citations
Reviews

Citations for Human IGF-I/IGF-1 Antibody (5)

Showing 1 - 5 of 5 publications Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Human IGF-I/IGF-1 Antibody include:

Species: Human
Sample Types: Cell Culture Lysates
Applications: Western Blot

(2020-12-17), 2020-10(0).
PMID: 33348564
Species: Human
Sample Types: Protein
Applications: Western Blot

Landowski C et al. (2015-08-26), Enabling Low Cost Biopharmaceuticals: A Systematic Approach to Delete Proteases from a Well-Known Protein Production Host Trichoderma reesei. PLoS ONE, 2015-010(8):e0134723.
PMID: 26309247
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Capture)

Luo X et al. (2014-06-17), ATM regulates insulin-like growth factor 1-secretory clusterin (IGF-1-sCLU) expression that protects cells against senescence. PLoS ONE, 2014-09(6):e99983.
PMID: 24937130
Species: Human
Sample Types: Serum
Applications: ELISA Microarray Development

Gonzalez RM et al. (2008-04-19), Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-07(6):2406-14.
PMID: 18422355
Species: Human
Sample Types: Plasma
Applications: ELISA Development

Yin D et al. (2007-02-01), Clinical pharmacodynamic effects of the growth hormone receptor antagonist pegvisomant: implications for cancer therapy. Clin. Cancer Res., 2007-013(3):1000-9.
PMID: 17289896

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Customer Reviews for Human IGF-I/IGF-1 Antibody (2)

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