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Human IGF-I/IGF-1 Antibody Best Seller

Catalog # MAB291 | R&D Systems, Inc. a Bio-Techne Brand
Catalog #
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Key Product Details

Species Reactivity



ELISA Capture (Matched Antibody Pair), Western Blot



Antibody Source

Monoclonal Mouse IgG1 Clone # 56408

Product Summary for Human IGF-I/IGF-1 Antibody


E. coli-derived recombinant human IGF-I/IGF-1
Accession # P01343


Detects human IGF-I/IGF-1 in ELISAs and Western blots. In ELISAs, this antibody does not cross-react with recombinant mouse (rm) IGF-I/IGF-1, rmIGF‑II/IGF-2, or recombinant human IGF‑II/IGF-2.







Scientific Data Images for Human IGF-I/IGF-1 Antibody

Detection of Recombinant Human IGF-I/IGF-1 antibody by Western Blot.

Detection of Recombinant Human IGF-I/IGF-1 by Western Blot.

Western blot shows 25 ng of Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1), Recombinant Mouse IGF-I/IGF-1 (Catalog # 791-MG), Recombinant Rat IGF-I/IGF-1 (Catalog # 4326-RG), and Recombinant Human IGF-II (Catalog # 292-G2). PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human IGF-I/IGF-1 Monoclonal Antibody (Catalog # MAB291) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IGF-I/IGF-1 at approximately 10 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human IGF-I/IGF-1 Antibody

Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1)

Human IGF-I/IGF-1 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human IGF-I/IGF-1 Biotinylated Antibody (Catalog # BAF291)
  • Standard: Recombinant Human IGF-I/IGF-1 Protein, CF (Catalog # 291-G1)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 5 using MAB291 in the following applications:

Published Applications

Read 5 publications using MAB291 in the following applications:

Formulation, Preparation and Storage


Protein A or G purified from ascites


Reconstitute at 0.5 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price


Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I/IGF-1

IGF-I/IGF-1 belongs to the family of insulin-like growth factors and circulates in complex with IGF binding proteins. It is a potent mitogenic growth factor that binds the heteromeric type I and type II IGF receptors. Essentially, all of the biological activities of IGF-I/IGF-1 are mediated by IGF-I R.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Entrez Gene IDs

3479 (Human); 16000 (Mouse); 24482 (Rat)

Gene Symbol



Product Documents for Human IGF-I/IGF-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human IGF-I/IGF-1 Antibody

For research use only

Citations for Human IGF-I/IGF-1 Antibody (5)

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Customer Reviews for Human IGF-I/IGF-1 Antibody (2)

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  • Human IGF-I/IGF-1 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: cells medium and HE49 cells
    Species: Human
    Verified Customer | Posted 06/18/2021
    Samples were normalized to cell number and the total volume of medium
    Human IGF-I/IGF-1 Antibody MAB291
  • Human IGF-I/IGF-1 Antibody
    Name: Anonymous
    Application: Immunohistochemistry
    Sample Tested: decidual
    Species: Human
    Verified Customer | Posted 04/10/2021
    Low primary antibody dilution (1:500) for IHC.
    Human IGF-I/IGF-1 Antibody MAB291

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Reconstitution Calculator

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