Recombinant Rat IGF-I/IGF-1 Protein, CF

Name: Recombinant Rat IGF-I/IGF-1 Protein, CF
Brand: R&D Systems
SKU: 4326-RG

Catalog # 4326-RG | R&D Systems, Inc. a Bio-Techne Brand

Citations (10)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Accession #:	AAA41386
Applications:	Bioactivity

View Full Technical Details for Recombinant Rat IGF-I/IGF-1 Protein, CF

Technical Data
Citations (10) / Reviews

Product Specifications for Recombinant Rat IGF-I/IGF-1 Protein, CF

Source

E. coli-derived rat IGF-I/IGF-1 protein

MHHHHHHN	Rat IGF-I/IGF-1 (Gly23-Ala92) Accession # AAA41386
N-terminus	C-terminus

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Met

Predicted Molecular Mass

8.8 kDa

SDS-PAGE

8.8 kDa

Activity

Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED₅₀ for this effect is 0.6-3 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

4326-RG

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IGF-I/IGF-1

Insulin-like growth factor I (IGF-I or IGF-1), also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Rat IGF-I/IGF-1 is synthesized as four precursor isoforms with alternate N- and C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I/IGF-1 is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature rat IGF-I/IGF-1 shares 96% and 99% aa sequence identity with human and mouse IGF-I/IGF-1, respectively (2, 3), and exhibits cross-species activity. It shares 61% aa sequence identity with mature rat IGF-II/IGF-2. Circulating IGF-I/IGF-1 is produced by hepatocytes, while local IGF-I/IGF-1 is produced by many other tissues in which it has paracrine effects (1). IGF-I/IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (4). IGF-I/IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (5 - 8). It plays an important role in muscle regeneration and tumor progression (1, 9). IGF-I/IGF-1 binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (10). Heterodimers of insulin receptor/IGF-I R also exist and respond to IGF-I/IGF-1 but not to insulin stimulation (11). IGF-I/IGF-1 association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (12).

Item	Value
References	Philippou, A. et al. (2007) In Vivo 21:45. Roberts, Jr., C.T. et al. (1987) Mol. Endocrinol. 1:243. Shimatsu, A. and P. Rotwein (1987) J. Biol. Chem. 262:7894. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275. Malemud, C.J. (2007) Clin. Chim. Acta 375:10. Samani, A.A. et al. (2007) Endocr. Rev. 28:20. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302. Benyoucef, S. et al. (2007) Biochem. J. 403:603. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
Long Name	Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Entrez Gene IDs	3479 (Human); 16000 (Mouse); 24482 (Rat)
Alternate Names	IBP1, IGF-1, IGF-I, IGF-IA, IGF-IB, IGF1, IGF1A, IGFI, MGF, Mechano growth factor, Somatomedin A, Somatomedin C, insulin-like growth factor 1, insulin-like growth factor 1 (somatomedin C), insulin-like growth factor I, insulin-like growth factor IA, insulin-like growth factor IB, somatomedin-C

Citations for Recombinant Rat IGF-I/IGF-1 Protein, CF (10)

Showing 1 - 10 of 10 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat IGF-I/IGF-1 Protein, CF include:

Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

B Karayay et al. (2023-02-27), Degranulation of Murine Resident Cochlear Mast Cells: A Possible Factor Contributing to Cisplatin-Induced Ototoxicity and Neurotoxicity International Journal of Molecular Sciences, 2023-024(5).
PMID: 36902051
Species: Mouse, Rat
Sample Types: Whole Cells
Applications: Bioassay

AJ Szczepek et al. (2020-10-01), Mast Cells in the Auditory Periphery of Rodents Brain Sci, 2020-110(10).
PMID: 33019672
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

X Ding et al. (2020-06-16), Serum IgG-induced microglial activation enhances neuronal cytolysis via the NO/sGC/PKG pathway in children with opsoclonus-myoclonus syndrome and neuroblastoma J Neuroinflammation, 2020-017(1):190.
PMID: 32546235
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

H Chen et al. (2019-06-29), Role of Programmed Cell Death 4 (PDCD4)-Mediated Akt Signaling Pathway in Vascular Endothelial Cell Injury Caused by Lower-Extremity Ischemia-Reperfusion in Rats Med. Sci. Monit., 2019-025(0):4811-4818.
PMID: 31253757
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

JJ Yuan et al. (2019-05-17), Young plasma ameliorates aging-related acute brain injury after intracerebral hemorrhage Biosci. Rep., 2019-00(0).
PMID: 31040201
Species: Rat
Sample Types: In Vivo, Whole Cells
Applications: Bioassay, In Vivo

H Zhu et al. (2018-06-18), miR-129 controls axonal regeneration via regulating insulin-like growth factor-1 in peripheral nerve injury Cell Death Dis, 2018-09(7):720.
PMID: 29915198
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Fornaro et al. (2013-11-19), Mechano-growth factor peptide, the COOH terminus of unprocessed insulin-like growth factor 1, has no apparent effect on myoblasts or primary muscle stem cells. Am J Physiol Endocrinol Metab, 2013-1306(2):E150-6.
PMID: 24253050
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

(2012-05-17), Insulin-like growth factor-1 lowers spreading depression susceptibility and reduces oxidative stress. J. Neurochem., 2012-0122(1):221-9.
PMID: 22524542
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Asumda FZ et al. (2011-10-12), Age-related changes in rat bone-marrow mesenchymal stem cell plasticity. BMC Cell Biol., 2011-112(0):44.
PMID: 21992089
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Gilbert RE et al. (2011-03-09), Histone deacetylase inhibition attenuates diabetes-associated kidney growth: potential role for epigenetic modification of the epidermal growth factor receptor. Kidney Int., 2011-079(12):1312-21.
PMID: 21389970

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