Human IGF-I/IGF-1 Biotinylated Antibody Best Seller

Catalog # BAF291 | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Species Reactivity

Human

Applications

ELISA Detection (Matched Antibody Pair), Simple Western, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

Product Summary for Human IGF-I/IGF-1 Biotinylated Antibody

Immunogen

E. coli-derived recombinant human IGF-I/IGF-1

Specificity

Detects human IGF-I/IGF-1 in ELISAs and Western blots. In sandwich immunoassays, less than 0.1% recombinant human (rh) IGF-I/IGF-1 sR, recombinant mouse (rm) IGF-I/IGF-1, rmIGF‑II/IGF-2, rhIGF-II/IGF-2, rhIGFBP-1, rhIGFBP-2, rhIGFBP-3, rhIGFBP-4, rhIGFBP-5, and rhIGFBP-6 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human IGF-I/IGF-1 Biotinylated Antibody

Detection of Human IGF-I/IGF-1 by Simple WesternTM.

Simple Western lane view shows lysates of Recombinant Human IGF-1, loaded at 0.2 mg/mL. A specific band was detected for IGF-I/IGF-1 at approximately 6 kDa (as indicated) using 50 µg/mL of Goat Anti-Human IGF-I/IGF-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF291) . This experiment was conducted under reducing conditions and using the 2-40kDa separation system.

Applications for Human IGF-I/IGF-1 Biotinylated Antibody

Application
Recommended Usage

Simple Western

50 µg/mL
Sample: Recombinant Human IGF-1

Western Blot

0.1 µg/mL
Sample: Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1)

Human IGF-I/IGF-1 Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.1-0.4 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human IGF-I/IGF-1 Antibody (Catalog # MAB291)
  • Standard: Recombinant Human IGF-I/IGF-1 Protein, CF (Catalog # 291-G1)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Published Applications

Read 5 publications using BAF291 in the following applications:

Formulation, Preparation and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-I/IGF-1

Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I/IGF-1 is synthesized as two precursor isoforms with N- and alternate C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I/IGF-1 is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature human IGF-I/IGF-1 shares 94% and 96% aa sequence identity with mouse and rat IGF-I/IGF-1, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF-II/IGF-2. Circulating IGF-I/IGF-1 is produced by hepatocytes, while local IGF-I/IGF-1 is produced by many other tissues in which it has paracrine effects (1). IGF-I/IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I/IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4 - 7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I/IGF-1 association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).

References

  1. Philippou, A. et al. (2007) In Vivo 21:45.
  2. Sandberg-Nordqvist, A.C. et al. (1992) Brain Res. Mol. Brain Res. 12:275.
  3. Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
  4. Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
  5. Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
  6. Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
  7. Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
  8. Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
  9. LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
  10. Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.

Long Name

Insulin-like Growth Factor I/Insulin-like Growth Factor 1

Alternate Names

IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C

Entrez Gene IDs

3479 (Human); 16000 (Mouse); 24482 (Rat)

Gene Symbol

IGF1

Product Documents for Human IGF-I/IGF-1 Biotinylated Antibody

Certificate of Analysis

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Product Specific Notices for Human IGF-I/IGF-1 Biotinylated Antibody

For research use only

Citations for Human IGF-I/IGF-1 Biotinylated Antibody (5)

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Reconstitution Calculator

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