Human IGF-I/IGF-1 Biotinylated Antibody Best Seller
Catalog # BAF291 | R&D Systems, Inc. a Bio-Techne Brand
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Antibody Source
Product Summary for Human IGF-I/IGF-1 Biotinylated Antibody
Immunogen
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Scientific Data Images for Human IGF-I/IGF-1 Biotinylated Antibody
Detection of Human IGF-I/IGF-1 by Simple WesternTM.
Simple Western lane view shows lysates of Recombinant Human IGF-1, loaded at 0.2 mg/mL. A specific band was detected for IGF-I/IGF-1 at approximately 6 kDa (as indicated) using 50 µg/mL of Goat Anti-Human IGF-I/IGF-1 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF291) . This experiment was conducted under reducing conditions and using the 2-40kDa separation system.Applications for Human IGF-I/IGF-1 Biotinylated Antibody
Simple Western
Sample: Recombinant Human IGF-1
Western Blot
Sample: Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1)
Human IGF-I/IGF-1 Sandwich Immunoassay
Published Applications
Read 5 publications using BAF291 in the following applications:
Formulation, Preparation and Storage
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Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGF-I/IGF-1
Insulin-like growth factor I, also known as somatomedin C, is the dominant effector of growth hormone and is structurally homologous to proinsulin. Human IGF-I/IGF-1 is synthesized as two precursor isoforms with N- and alternate C-terminal propeptides (1). These isoforms are differentially expressed by various tissues (1). The 7.6 kDa mature IGF-I/IGF-1 is identical between isoforms and is generated by proteolytic removal of the N- and C-terminal regions. Mature human IGF-I/IGF-1 shares 94% and 96% aa sequence identity with mouse and rat IGF-I/IGF-1, respectively (2), and exhibits cross-species activity. It shares 64% aa sequence identity with mature human IGF-II/IGF-2. Circulating IGF-I/IGF-1 is produced by hepatocytes, while local IGF-I/IGF-1 is produced by many other tissues in which it has paracrine effects (1). IGF-I/IGF-1 induces the proliferation, migration, and differentiation of a wide variety of cell types during development and postnatally (3). IGF-I/IGF-1 regulates glucose and fatty acid metabolism, steroid hormone activity, and cartilage and bone metabolism (4 - 7). It plays an important role in muscle regeneration and tumor progression (1, 8). IGF-I binds IGF-I R, IGF-II R, and the insulin receptor, although its effects are mediated primarily by IGF-I R (9). IGF-I/IGF-1 association with IGF binding proteins increases its plasma half-life and modulates its interactions with receptors (10).
References
- Philippou, A. et al. (2007) In Vivo 21:45.
- Sandberg-Nordqvist, A.C. et al. (1992) Brain Res. Mol. Brain Res. 12:275.
- Guvakova, M.A. (2007) Int. J. Biochem. Cell Biol. 39:890.
- Clemmons, D.R. (2006) Curr. Opin. Pharmacol. 6:620.
- Bluher, S. et al. (2005) Best Pract. Res. Clin. Endocrinol. Metab. 19:577.
- Garcia-Segura, L.M. et al. (2006) Neuroendocrinology 84:275.
- Malemud, C.J. (2007) Clin. Chim. Acta 375:10.
- Samani, A.A. et al. (2007) Endocrine Rev. 28:20.
- LeRoith, D. and S. Yakar (2007) Nat. Clin. Pract. Endocrinol. Metab. 3:302.
- Denley, A. et al. (2005) Cytokine Growth Factor Rev. 16:421.
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Product Documents for Human IGF-I/IGF-1 Biotinylated Antibody
Product Specific Notices for Human IGF-I/IGF-1 Biotinylated Antibody
For research use only
Citations for Human IGF-I/IGF-1 Biotinylated Antibody (5)
Citations are publications that use Bio-Techne products. Selected citations for Human IGF-I/IGF-1 Biotinylated Antibody include:
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Species: Mouse
Sample Types: Serum
Applications: ELISA DetectionJ Li et al. (2018-02-14), Tough Composite Hydrogels with High Loading and Local Release of Biological Drugs Adv Healthc Mater, 2018-00(0).
PMID: 29441702 -
Species: Bovine
Sample Types: Milk
Applications: Array DevelopmentLudwig S et al. (2015-08-26), Calling Biomarkers in Milk Using a Protein Microarray on Your Smartphone. PLoS ONE, 2015-010(8):e0134360.
PMID: 26308444 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)Luo X et al. (2014-06-17), ATM regulates insulin-like growth factor 1-secretory clusterin (IGF-1-sCLU) expression that protects cells against senescence. PLoS ONE, 2014-09(6):e99983.
PMID: 24937130 -
Species: Human
Sample Types: Serum
Applications: ELISA Microarray DevelopmentGonzalez RM et al. (2008-04-19), Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-07(6):2406-14.
PMID: 18422355 -
Species: Human
Sample Types: Plasma
Applications: ELISA DevelopmentYin D et al. (2007-02-01), Clinical pharmacodynamic effects of the growth hormone receptor antagonist pegvisomant: implications for cancer therapy. Clin. Cancer Res., 2007-013(3):1000-9.
PMID: 17289896
There are no citations that match your criteria.
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