Human IGF-I/IGF-1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF-291-NA
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse, Rat, Porcine, Bovine
Applications
Validated:
Immunohistochemistry, Western Blot, Neutralization, Immunocytochemistry, Simple Western
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Neutralization, Immunocytochemistry, Immunoprecipitation, ELISA Development, ELISA Development (Detection), Neutralizing
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human IGF-I/IGF-1
Specificity
Detects human IGF-I/IGF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IGF-I/IGF-1 and recombinant rat IGF-I/IGF-1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human IGF-I/IGF-1 Antibody
Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Human IGF-I/IGF-1 Antibody.
Recombinant Human IGF-I/IGF-1 (291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-I/IGF-1 (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA). The ND50 is typically 3-12 µg/mL.
IGF-I/IGF-1 in MDA‑MB‑231 Human Cell Line.
IGF-I/IGF-1 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
IGF-I/IGF-1 in Human Placenta.
IGF-I/IGF-1 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human IGF-I/IGF-1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta (chorionic villi) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human placenta (chorionic villi) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Simple Western
50 µg/mL
Sample: Recombinant Human IGF-1
Sample: Recombinant Human IGF-1
Western Blot
0.1 µg/mL
Sample: Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1)
Sample: Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1)
Neutralization
Measured by its ability to neutralize IGF-I/IGF-1-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 6 ng/mL Recombinant Human IGF-I/IGF-1.
Reviewed Applications
Read 4 reviews rated 4.5 using AF-291-NA in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGF-I/IGF-1
Long Name
Insulin-like Growth Factor I/Insulin-like Growth Factor 1
Alternate Names
IGF-1, IGF1, IGFI, Somatomedin A, Somatomedin C
Gene Symbol
IGF1
Additional IGF-I/IGF-1 Products
Product Documents for Human IGF-I/IGF-1 Antibody
Product Specific Notices for Human IGF-I/IGF-1 Antibody
For research use only
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