Key Product Details
Product Summary for Human IGF-I/IGF-1 Antibody
Scientific Data Images for Human IGF-I/IGF-1 Antibody
Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Human IGF-I/IGF-1 Antibody.Recombinant Human IGF-I/IGF-1 (291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-I/IGF-1 (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA). The ND50 is typically 3-12 µg/mL.
IGF-I/IGF-1 in MDA‑MB‑231 Human Cell Line.IGF-I/IGF-1 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
IGF-I/IGF-1 in Human Placenta.IGF-I/IGF-1 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Applications for Human IGF-I/IGF-1 Antibody
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line
Sample: Immersion fixed paraffin-embedded sections of human placenta (chorionic villi) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Recombinant Human IGF-1
Sample: Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1)
Read 4 reviews rated 4.5 using AF-291-NA in the following applications:
- Immunocytochemistry/Immunofluorescence (1 Review)
- Siteclcik antibody biotinylation (1 Review)
- Western Blot (2 Reviews)
Read 27 publications using AF-291-NA in the following applications:
Formulation, Preparation and Storage
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Product Specific Notices for Human IGF-I/IGF-1 Antibody
For research use only