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Human IGF-I/IGF-1 Antibody
Catalog # AF-291-NA | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Neutralization, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Summary for Human IGF-I/IGF-1 Antibody
Immunogen
E. coli-derived recombinant human IGF-I/IGF-1
Specificity
Detects human IGF-I/IGF-1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant mouse IGF-I/IGF-1 and recombinant rat IGF-I/IGF-1 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human IGF-I/IGF-1 Antibody
Cell Proliferation Induced by IGF-I/IGF-1 and Neutralization by Human IGF-I/IGF-1 Antibody.
Recombinant Human IGF-I/IGF-1 (291-G1) stimulates proliferation in the MCF-7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IGF-I/IGF-1 (6 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA). The ND50 is typically 3-12 µg/mL.
IGF-I/IGF-1 in MDA‑MB‑231 Human Cell Line.
IGF-I/IGF-1 was detected in immersion fixed MDA-MB-231 human breast cancer cell line using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
IGF-I/IGF-1 in Human Placenta.
IGF-I/IGF-1 was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human IGF-I/IGF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-291-NA) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to syncytiotrophoblasts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human IGF-I/IGF-1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line
Sample: Immersion fixed MDA-MB-231 human breast cancer cell line
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human placenta (chorionic villi) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Sample: Immersion fixed paraffin-embedded sections of human placenta (chorionic villi) subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)
Simple Western
50 µg/mL
Sample: Recombinant Human IGF-1
Sample: Recombinant Human IGF-1
Western Blot
0.1 µg/mL
Sample: Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1)
Sample: Recombinant Human IGF-I/IGF-1 (Catalog # 291-G1)
Neutralization
Measured by its ability to neutralize IGF-I/IGF-1-induced proliferation in the MCF‑7 human breast cancer cell line. Karey, K.P. et al. (1988) Cancer Research 48:4083. The Neutralization Dose (ND50) is typically 3-12 µg/mL in the presence of 6 ng/mL Recombinant Human IGF-I/IGF-1.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 4 reviews rated 4.5 using AF-291-NA in the following applications:
- Immunocytochemistry/Immunofluorescence (1 Review)
- Siteclcik antibody biotinylation (1 Review)
- Western Blot (2 Reviews)
Published Applications
Read 27 publications using AF-291-NA in the following applications:
Formulation, Preparation and Storage
Purification
Antigen Affinity-purified
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IGF-I/IGF-1
Product Documents for Human IGF-I/IGF-1 Antibody
Product Specific Notices for Human IGF-I/IGF-1 Antibody
For research use only
Citations for Human IGF-I/IGF-1 Antibody (27)
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Citations are publications that use Bio-Techne products. Selected citations for Human IGF-I/IGF-1 Antibody include:
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Species: Rat
Sample Types: Whole Tissue
Applications: IHCGuan et al. (2023-07-27), Reversible Effects of Functional Mandibular Lateral Shift on Masticatory Muscles in Growing Rats Biomedicines
PMID: 37626623 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationKM Lugo-Cintr et al. (2021-10-18), Primary Head and Neck Tumour-Derived Fibroblasts Promote Lymphangiogenesis in a Lymphatic Organotypic Co-culture Model EBioMedicine, 2021-173(0):103634.
PMID: 34673450 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotQ Zhang et al. (2020-06-17), lncRNA NR2F1-AS1 promotes breast cancer angiogenesis through activating IGF-1/IGF-1R/ERK pathway J. Cell. Mol. Med., 2020-00(0).
PMID: 32548873 -
Species: Human
Sample Types: Whole Tissue
Applications: IHCF Alvarez-Ri et al. (2020-04-09), Controlled Release of rAAV Vectors from APMA-Functionalized Contact Lenses for Corneal Gene Therapy Pharmaceutics, 2020-012(4).
PMID: 32283694 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationS Lie et al. (2019-09-29), Molecular Basis of The Retinal Pigment Epithelial Changes That Characterize The Ocular Lesion in Toxoplasmosis Microorganisms, 2019-07(10).
PMID: 31569536 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PS Morscheid et al. (2019-05-27), Therapeutic Effects of rAAV-Mediated Concomittant Gene Transfer and Overexpression of TGF-? and IGF-I on the Chondrogenesis of Human Bone-Marrow-Derived Mesenchymal Stem Cells Int J Mol Sci, 2019-020(10).
PMID: 31137788 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationM Paku?a et al. (2019-05-13), A Unique Pattern of Mesothelial-Mesenchymal Transition Induced in the Normal Peritoneal Mesothelium by High-Grade Serous Ovarian Cancer Cancers (Basel), 2019-011(5).
PMID: 31086083 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCA Pristerà et al. (2019-02-11), Dopamine neuron-derived IGF-1 controls dopamine neuron firing, skill learning, and exploration Proc. Natl. Acad. Sci. U.S.A., 2019-0116(9):3817-3826.
PMID: 30808767 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationM Paku?a et al. (2019-01-02), The Epithelial-Mesenchymal Transition Initiated by Malignant Ascites Underlies the Transmesothelial Invasion of Ovarian Cancer Cells Int J Mol Sci, 2019-020(1).
PMID: 30609691 -
Species: Mouse
Sample Types: Whole Tissue
Applications: IHCL Dou et al. (2018-10-19), The effect of cinnamon on polycystic ovary syndrome in a mouse model Reprod. Biol. Endocrinol., 2018-116(1):99.
PMID: 30340496 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationD Wang et al. (2017-12-07), Protein C receptor stimulates multiple signaling pathways in breast cancer cells J. Biol. Chem., 2017-10(0).
PMID: 29217770 -
Species: Mouse
Sample Types: Whole Cells
Applications: NeutralizationL Song et al. (2017-08-30), Adipose stem cells from chronic pancreatitis patients improve mouse and human islet survival and function Stem Cell Res Ther, 2017-08(1):192.
PMID: 28854965 -
Species: Human
Sample Types: Whole Cells
Applications: ICCJ Frisch et al. (2017-05-03), Peripheral blood aspirates overexpressing IGF-I via rAAV gene transfer undergo enhanced chondrogenic differentiation processes J. Cell. Mol. Med., 2017-00(0).
PMID: 28467017 -
Species: Human
Sample Types: Whole Tissue
Applications: IHC-PMagali Cucchiarin (2015-07-22), Chondrogenic Differentiation Processes in Human Bone Marrow Aspirates upon rAAV-Mediated Gene Transfer and Overexpression of the Insulin-Like Growth Factor I Tissue Eng Part A, 2015-021(17):2460-71.
PMID: 26123891 -
Species: Human
Sample Types: Whole Cells
Applications: ICCBenabbou N et al. (2014-07-29), A subset of bone marrow stromal cells regulate ATP-binding cassette gene expression via insulin-like growth factor-I in a leukemia cell line. Int J Oncol, 2014-045(4):1372-80.
PMID: 25095896 -
Species: Human
Sample Types: Cell Culture Supernates
Applications: ELISA Development (Detection)Luo X et al. (2014-06-17), ATM regulates insulin-like growth factor 1-secretory clusterin (IGF-1-sCLU) expression that protects cells against senescence. PLoS ONE, 2014-09(6):e99983.
PMID: 24937130 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationDu et al. (2014-01-27), Hypoxia enhances protective effect of placental-derived mesenchymal stem cells on damaged intestinal epithelial cells by promoting secretion of insulin-like growth factor-1. Int J Mol Sci, 2014-015(2):1983-2002.
PMID: 24473145 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationNi et al. (2013-01-01), IGF-1 promotes the development and cytotoxic activity of human NK cells. Nat Commun, 2013-04(0):1479.
PMID: 23403580 -
Species: Human
Sample Types: Cell Lysates,Whole Cells
Applications: Western Blot,ICCWeimer A et al. (2012-05-09), Benefits of recombinant adeno-associated virus (rAAV)-mediated insulinlike growth factor I (IGF-I) overexpression for the long-term reconstruction of human osteoarthritic cartilage by modulation of the IGF-I axis. Mol. Med., 2012-018(1):346-58.
PMID: 22160392 -
Species: Human
Sample Types: Cell Culture Supernates,Cell Lysates,Whole Cells
Applications: ELISA Development,Immunoprecipitation,Western Blot,NeutralizationBanziger-Tobler NE et al. (2008-06-26), Growth hormone promotes lymphangiogenesis. Am. J. Pathol., 2008-0173(2):586-97.
PMID: 18583315 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationSkibinski G et al. (2007-03-23), Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor. Am. J. Physiol. Lung Cell Mol. Physiol., 2007-0293(1):L69-76.
PMID: 17384084 -
Species: Bovine
Sample Types: Cell Lysates
Applications: Western BlotYoon DM et al. (2006-09-18), Effect of construct properties on encapsulated chondrocyte expression of insulin-like growth factor-1. Biomaterials, 2006-028(2):299-306.
PMID: 16982090 -
Species: Human
Sample Types: Whole Cells
Applications: NeutralizationSchnapp LM et al. (2006-07-01), Mining the acute respiratory distress syndrome proteome: identification of the insulin-like growth factor (IGF)/IGF-binding protein-3 pathway in acute lung injury. Am. J. Pathol., 2006-0169(1):86-95.
PMID: 16816363 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotMcCaig C et al. (2006-03-06), The role of matrix metalloproteinase-7 in redefining the gastric microenvironment in response to Helicobacter pylori. Gastroenterology, 2006-0130(6):1754-63.
PMID: 16697739 -
Species: Human
Sample Types: Cell Lysates
Applications: Western BlotLee JH et al. (2006-03-01), Induced expression of insulin-like growth factor-1 by amniotic membrane-conditioned medium in cultured human corneal epithelial cells. Invest. Ophthalmol. Vis. Sci., 2006-047(3):864-72.
PMID: 16505018 -
Species: Human
Sample Types: Tissue Homogenates,Whole Tissue
Applications: Western Blot,IHC-FrWade KC et al. (2006-02-10), Gene induction during differentiation of human pulmonary type II cells in vitro. Am. J. Respir. Cell Mol. Biol., 2006-034(6):727-37.
PMID: 16474099 -
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-PBrenner N et al. (2004-04-01), Quantification, localization, and expression of IGF-I and TGF-beta1 during growth factor-stimulated fracture healing. Calcif. Tissue Int., 2004-074(4):388-97.
PMID: 15255077
There are no citations that match your criteria.
Customer Reviews for Human IGF-I/IGF-1 Antibody (4)
4.5 out of 5
4 customer
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Application: Immunocytochemistry/ImmunofluorescenceSample Tested: Pancreas tissueSpecies: CanineVerified Customer | Posted 03/08/2022Used at 1:50 concentration in Casein in PBS overnight at 4 C. HIER was performed for two hours at 75 C in a citrate buffer.
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Application: Siteclcik antibody biotinylationSample Tested: B16-F1 mouse melanoma cell lineSpecies: MouseVerified Customer | Posted 10/27/2017
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Application: Western BlotSample Tested: See PMID 24036101Species: HumanVerified Customer | Posted 01/07/2015
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Application: Western BlotSample Tested: See PMID 23424195Species: HumanVerified Customer | Posted 01/07/2015
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