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Recombinant Human IGF-II/IGF2 Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Recombinant Human IGF-II/IGF2 Protein Bioactivity
(2)
Recombinant Human IGF-II/IGF2 Protein SDS-PAGE
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292-G2-250
292-G2-050
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Key Product Details

Source: E. coli
Accession #: P01344.1
Applications: Bioactivity

Citations for Recombinant Human IGF-II/IGF2 Protein, CF (40)

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Customer Reviews for Recombinant Human IGF-II/IGF2 Protein, CF (1)

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  • Recombinant Human IGF-II Protein, CF
    Name: Anonymous
    Application: Binding inhibition assay. I used it as a competitor to saturate the IGF2 binding site to block the IGF2 receptor-mediated endocytosis.
    Verified Customer | Posted 08/03/2016
    Cells were seeded in 12-well plates and grown to confluence. Culture medium was replaced with 0.5 ml of EMEM without serum prior to the addition of uptake inhibitors. 5 µg/ml recombinant human IGF-II (R&D Systems; Minneapolis, MN) was applied to the cells for 10 minutes prior to applying purified rhNAGLU-IGF-II at a final concentration of 160 units/ml. Following 4 hours incubation at 37°C and 5% CO2, cells were harvested and intracellular NAGLU activity was measured as described above. The enzyme activity measured in MPS IIIB fibroblasts with rhNAGLU-IGF-II treatment alone was defined as 100% and the intracellular enzyme activity observed in the presence of inhibitor was normalized to rhNAGLU-IGF-II activity. (Biochem J. 2014 458(2):281-9.)

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Product Documents for Recombinant Human IGF-II/IGF2 Protein, CF

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