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Recombinant Human IGF-II/IGF2 Protein, CF Best Seller

Catalog # 292-G2 | R&D Systems, Inc. a Bio-Techne Brand
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292-G2-050
292-G2-250

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived human IGF-II/IGF2 protein
Ala25-Glu91

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala25

Predicted Molecular Mass

7.5 kDa

Activity

Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083.
The ED50 for this effect is 1.5‑6 ng/mL.

Scientific Data Images for Recombinant Human IGF-II/IGF2 Protein, CF

Recombinant Human IGF-II/IGF2 Protein Bioactivity

Recombinant Human IGF-II/IGF2 Protein Bioactivity

Recombinant Human IGF-II/IGF2 (Catalog # 292‑G2) stimulates cell proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 1.5‑6 ng/mL.
Recombinant Human IGF-II/IGF2 Protein SDS-PAGE

Recombinant Human IGF-II/IGF2 Protein SDS-PAGE

1 μg/lane of Recombinant Human IGF-II/IGF2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.

Formulation, Preparation and Storage

292-G2
Formulation Lyophilized from a 0.2 μm filtered solution in Acetic Acid.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGF-II/IGF2

Insulin-like growth factor I (also known as somatomedin C and somatomedin A) and insulin-like growth factor II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved (100% identity between human, bovine and porcine proteins) and exhibit cross-species activity.

IGF-II is a potent mitogenic growth factor. However, unlike IGF-I which has important postnatal roles, the growth-promoting function of IGF-II is limited to embryonic development.

Two specific cell surface receptors that bind IGF-I and IGF-II have been identified. The type I IGF receptor that participates in IGF signaling is structurally related to the insulin receptor. It is a disulfide-linked heterotetrameric transmembrane glycoprotein with an intracellular tyrosine kinase domain. Type I IGF receptor binds IGF-I with higher affinity than IGF-II. The type II IGF receptor which binds IGF-II with much higher affinity than IGF-I is also the cation-independent mannose 6-phosphate receptor. At the present time, it is not known if the type II IGF receptor participates in the IGF signaling pathway. An additional unknown receptor which mediates IGF‑II signaling has also been proposed. Circulating IGFs exist in complexes bound to IGF binding proteins. Currently, at least six high affinity binding proteins have been identified.

Long Name

Insulin-like Growth Factor II

Alternate Names

C11orf43, GRDF, IGF-2, IGF2, IGFII, MSA, PEG2, PP9974

Entrez Gene IDs

3481 (Human); 16002 (Mouse)

Gene Symbol

IGF2

UniProt

Product Documents for Recombinant Human IGF-II/IGF2 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human IGF-II/IGF2 Protein, CF

For research use only

Citations for Recombinant Human IGF-II/IGF2 Protein, CF (44)

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  • Recombinant Human IGF-II Protein, CF
    Name: Anonymous
    Application: Binding Inhibition
    Verified Customer | Posted 08/03/2016
    Cells were seeded in 12-well plates and grown to confluence. Culture medium was replaced with 0.5 ml of EMEM without serum prior to the addition of uptake inhibitors. 5 µg/ml recombinant human IGF-II (R&D Systems; Minneapolis, MN) was applied to the cells for 10 minutes prior to applying purified rhNAGLU-IGF-II at a final concentration of 160 units/ml. Following 4 hours incubation at 37°C and 5% CO2, cells were harvested and intracellular NAGLU activity was measured as described above. The enzyme activity measured in MPS IIIB fibroblasts with rhNAGLU-IGF-II treatment alone was defined as 100% and the intracellular enzyme activity observed in the presence of inhibitor was normalized to rhNAGLU-IGF-II activity. (Biochem J. 2014 458(2):281-9.) I used it as a competitor to saturate the IGF2 binding site to block the IGF2 receptor-mediated endocytosis.

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