Human Endoglin/CD105 Antibody

Name: Human Endoglin/CD105 Antibody
Brand: R&D Systems
SKU: AF1097
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF1097

Citations (21)

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Key Product Details

Species Reactivity:	Human
Immunogen:	Mouse myeloma cell line NS0-derived recombinant human Endoglin/CD105 Glu26-Gly586 Accession # Q5T9B9
Applications:	CyTOF-ready, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Knockout Validated, Simple Western, Western Blot
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects human Endoglin in direct ELISAs and Western blots.

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Applications for Human Endoglin/CD105 Antibody

	Recommended Concentration	Sample
Western Blot	0.25 µg/mL	See below
Simple Western	20 µg/mL	See below
Flow Cytometry	0.25 µg/10⁶ cells	U937 human histiocytic lymphoma cell line
Immunohistochemistry	5-15 µg/mL	See below
CyTOF-ready	Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunocytochemistry	5-15 µg/mL	See below

Item	Value
Knockout Validated	Endoglin/CD105 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Endoglin/CD105 knockout HeLa cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Human Endoglin/CD105 Antibody

	Western Blot Detection of Human Endoglin/CD105 by Western Blot. Western blot shows lysates of human kidney tissue, human lung tissue, human placenta tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Endoglin/CD105 at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
	Immunocytochemistry Endoglin/CD105 in U937 Human Cell Line. Endoglin/CD105 was detected in immersion fixed U937 human histiocytic lymphoma cell line using Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
	Immunohistochemistry Endoglin/CD105 in Human Prostate. Endoglin/CD105 was detected in immersion fixed paraffin-embedded sections of human prostate using Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of immersion fixed paraffin-embedded Tissue Sections.
	Immunohistochemistry Endoglin/CD105 in Mouse Kidney. Endoglin/CD105 was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membranes in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
	Simple Western Detection of Human Endoglin/CD105 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Endoglin/CD105 knockout HeLa cell line (KO), loaded at 0.2 mg/mL. A specific band was detected for Endoglin/CD105 at approximately 121 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) was used at 20 µg/mL followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
	Knockout Validated Western Blot Shows Human Endoglin/CD105 Specificity by Using Knockout Cell Line. Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Endoglin/CD105 knockout HeLa cell line (KO). PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Human Endoglin/CD105 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1097) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Endoglin/CD105 at approximately 90 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: Endoglin/CD105

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1-3). Endoglin and Betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2-5). Human endoglin cDNA encodes 658 amino acids including a 25 amino acid (aa) signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two-part ZP domain, a TM domain, and a 47 aa cytoplasmic domain (1-3). An isoform with a 14 aa cytoplasmic domain (S-endoglin) can oppose effects of long (L) endoglin (6, 7). The human endoglin ECD shares 65-72% aa identity with mouse, rat, bovine, porcine, and canine endoglin. Endoglin homodimers interact with TGF-beta 1 and TGF-beta 3 (but not TGF-beta 2) but only after binding T beta RII (8). Similarly, they interact with activin-A and BMP-7 via activin type IIA or B receptors, and with BMP-2 via BMPR-1A/ALK-3 or BMPR-1B/ALK-6 (9). BMP-9, however, is reported to bind endoglin directly (10). Endoglin modifies ligand-induced signaling in multiple ways. For example, expression of endoglin can inhibit TGF-beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, endoglin inhibits T beta RI/ALK5, but enhances ALK1-mediated activation (12). Deletion of mouse endoglin causes lethal vascular and cardiovascular defects, and human endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2-4, 12-14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble endoglin and VEGF R1 (sFlt-1), along with low Placental Growth Factor (PlGF), are pathogenic due to antiangiogenic activity (15).

Item	Value
References	Gougos, A. and Letarte, M. (1990) J. Biol. Chem. 265:8361. ten Dijke, P. et al. (2008) Angiogenesis 11:79. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375. Mancini, M.L. et al. (2007) Dev. Biol. 308:520. Moody, J.L. et al. (2007) Stem Cells 25:2809. Velasco, S. et al. (2008) J. Cell Sci. 121:913. Perez-Gomez, E. et al. (2005) Oncogene 24:4450. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800. Arthur, H.M. et al. (2000) Dev. Biol. 217:42. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25. Venkatesha, S. et al. (2006) Nat. Med. 12:642.
Entrez Gene IDs	2022 (Human); 13805 (Mouse); 497010 (Rat)
Alternate Names	CD105, CD105 antigen, ENDOsler-Rendu-Weber syndrome 1, ENG, Endoglin, HHT1FLJ41744, ORW, ORW1

Citations for Human Endoglin/CD105 Antibody (21)

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Citations are publications that use Bio-Techne products. Selected citations for Human Endoglin/CD105 Antibody include:

Species: Human
Sample Types: Whole Tissue
Applications: IHC

DN Mitroi et al. (2022), Single-nucleus transcriptome analysis reveals disease- and regeneration-associated endothelial cells in white matter vascular dementia Journal of Cellular and Molecular Medicine, 0(0).
PMID: 35543222
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

PL Mendez et al. (2022), Atheroprone fluid shear stress-regulated ALK1-Endoglin-SMAD signaling originates from early endosomes Bmc Biology, 20(1):210.
PMID: 36171573
Species: Mouse
Sample Types: Cell Lysates
Applications: Western Blot

G Galaris et al. (2021), Thresholds of Endoglin Expression in Endothelial Cells Explains Vascular Etiology in Hereditary Hemorrhagic Telangiectasia Type 1 International Journal of Molecular Sciences, 22(16).
PMID: 34445652
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

JV Korpershoe et al. (2021), Selection of Highly Proliferative and Multipotent Meniscus Progenitors through Differential Adhesion to Fibronectin: A Novel Approach in Meniscus Tissue Engineering International Journal of Molecular Sciences, 22(16).
PMID: 34445320
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

CSB Singh et al. (2021), Reversing pathology in a preclinical model of Alzheimer's disease by hacking cerebrovascular neoangiogenesis with advanced cancer therapeutics EBioMedicine, 71(0):103503.
PMID: 34534764
Species: Human
Sample Types: Whole Cells
Applications: ICC

KJG Kenswil et al. (2021), Endothelium-derived stromal cells contribute to hematopoietic bone marrow niche formation Cell Stem Cell, 0(0).
PMID: 33561425
Species: Human
Sample Types: Whole Tissue
Applications: IHC

T Gerrits et al. (2020), Endoglin Promotes Myofibroblast Differentiation and Extracellular Matrix Production in Diabetic Nephropathy Int J Mol Sci, 21(20).
PMID: 33081058
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Q Zhang et al. (2018), CD90 determined two subpopulations of glioma-associated mesenchymal stem cells with different roles in tumour progression Cell Death Dis, 9(11):1101.
PMID: 30368520
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry

HF Moffett et al. (2017), Hit-and-run programming of therapeutic cytoreagents using mRNA nanocarriers Nat Commun, 8(1):389.
PMID: 28855514
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Y Jin et al. (2017), Endoglin prevents vascular malformation by regulating flow-induced cell migration and specification through VEGFR2 signalling Nat. Cell Biol., 0(0).
PMID: 28530660
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

ML García-Her et al. (2017), A Unique Cellular and Molecular Microenvironment Is Present in Tertiary Lymphoid Organs of Patients with Spontaneous Prostate Cancer Regression Front Immunol, 8(0):563.
PMID: 28567040
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

(2016), Novel Rat Model of Repetitive Portal Venous Embolization Mimicking Human Non-Cirrhotic Idiopathic Portal Hypertension PLoS ONE, 11(9):e0162144.
PMID: 27589391
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

(2016), Evaluation of Nonperfused Retinal Vessels in Ischemic Retinopathy Invest Ophthalmol Vis Sci, 57(11):5031-5037.
PMID: 27661854
Species: Human
Sample Types: Whole Cells
Applications: IHC

(2016), Altered Biomarkers in Trophoblast Cells Obtained Noninvasively Prior to Clinical Manifestation of Perinatal Disease Sci Rep, 6(0):32382.
PMID: 27660926
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Riordan D et al. (2015), Automated Analysis and Classification of Histological Tissue Features by Multi-Dimensional Microscopic Molecular Profiling. PLoS ONE, 10(7):e0128975.
PMID: 26176839
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Martinez L et al. (2015), CD105 expression on CD34-negative spindle-shaped stromal cells of primary tumor is an unfavorable prognostic marker in early breast cancer patients. PLoS ONE, 10(3):e0121421.
PMID: 25803686
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Kim J et al. (2015), Umbilical cord mesenchymal stromal cells affected by gestational diabetes mellitus display premature aging and mitochondrial dysfunction. Stem Cells Dev, 24(5):575-86.
PMID: 25437179
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot

Gore B et al. (2014), Key role of the endothelial TGF-beta/ALK1/endoglin signaling pathway in humans and rodents pulmonary hypertension. PLoS ONE, 9(6):e100310.
PMID: 24956016
Species: Human
Sample Types: Whole Tissue
Applications: IHC

Boonstra M et al. (2014), Expression of uPAR in tumor-associated stromal cells is associated with colorectal cancer patient prognosis: a TMA study. BMC Cancer, 14(0):269.
PMID: 24742002
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P

Barsoum IB et al. (2011), Glyceryl trinitrate inhibits hypoxia-induced release of soluble fms-like tyrosine kinase-1 and endoglin from placental tissues. Am. J. Pathol., 178(6):2888-96.
PMID: 21641407
Species: Human
Sample Types: Whole Cells
Applications: ICC

Glavaski-Joksimovic A et al. (2010), Glial cell line-derived neurotrophic factor-secreting genetically modified human bone marrow-derived mesenchymal stem cells promote recovery in a rat model of Parkinson's disease. J. Neurosci. Res., 88(12):2669-81.
PMID: 20544825

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Human Endoglin/CD105 Antibody

Name: Anonymous

Application: WB

Sample Tested: Pancreatic cancer cells

Species: Human

Verified Customer | Posted 12/11/2019

Lysates and supernatants of pancreatic cancer cells AsPC-1 and MIA PaCa-2 were examined under denaturing/reducing conditions by SDS-PAGE/western blots and subsequent antibody stainings. Nitrocellulose membrane was probed with 0.13 ug/ml. This antibody stains endoglin as expected.

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Product Documents for Human Endoglin/CD105 Antibody

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Human Endoglin/CD105 Antibody

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Applications for Human Endoglin/CD105 Antibody

Scientific Data Examples for Human Endoglin/CD105 Antibody

Western Blot

Immunocytochemistry

Immunohistochemistry

Immunohistochemistry

Simple Western

Knockout Validated

Formulation, Preparation and Storage

Reconstitution Calculator

Background: Endoglin/CD105

Citations for Human Endoglin/CD105 Antibody (21)

Customer Reviews for Human Endoglin/CD105 Antibody (1)

Customer Images

Human Endoglin/CD105 Antibody

Product Documents for Human Endoglin/CD105 Antibody

Product Datasheets

COA

Certificate of Analysis

SDS

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