Recombinant Human Endoglin/CD105 Protein

Name: Recombinant Human Endoglin/CD105 Protein
Brand: R&D Systems
SKU: 1097-EN

Catalog # 1097-EN

Citations (8)

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New Version Available - 6578-EN

A New rh Endoglin is Available! It has ~100 fold better TGFbRI binding activity and has a Fc tag!

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Key Product Details

Source:	NS0
Accession #:	Q5T9C0
Applications:	Bioactivity

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Product Specifications

Source	Mouse myeloma cell line, NS0-derived human Endoglin/CD105 protein Glu26-Gly586
Purity	>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Glu26
Predicted Molecular Mass	61 kDa
SDS-PAGE	75-85 kDa, reducing conditions
Activity	Measured by its ability to inhibit BMP-10-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 0.06-0.36 μg/mL in the presence of 100 ng/mL of Recombinant Human BMP‑10 (Catalog # 2926-BP).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1097-EN

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1097-EN/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Endoglin/CD105

Endoglin (CD105) is a 90 kDa type I transmembrane glycoprotein of the zona pellucida (ZP) family of proteins (1‑3). Endoglin and betaglycan/T beta RIII are type III receptors for TGF beta superfamily ligands, sharing 71% aa identity in the transmembrane (TM) and cytoplasmic domains. Endoglin is highly expressed on proliferating vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta, with lower amounts on hematopoietic, mesenchymal and neural crest stem cells, activated monocytes, and lymphoid and myeloid leukemic cells (2 ‑ 5). Human endoglin cDNA encodes 658 amino acids (aa) including a 25 aa signal sequence, a 561 aa extracellular domain (ECD) with an orphan domain and a two‑part ZP domain, a TM domain and a 47 aa cytoplasmic domain (1‑3). An isoform with a 14 aa cytoplasmic domain (S‑endoglin) can oppose effects of long (L) endoglin (6, 7). The human endoglin ECD shares 65‑72% aa identity with mouse, rat, bovine, porcine and canine endoglin. Endoglin homodimers interact with TGF‑ beta 1 and TGF‑ beta 3 (but not TGF‑ beta 2), but only after binding T beta RII (8). Similarly, they interact with activin‑A and BMP‑7 via activin type IIA or B receptors, and with BMP‑2 via BMPR‑1A/ALK‑3 or BMPR‑1B/ALK‑6 (9). BMP‑9, however, is reported to bind endoglin directly (10). Endoglin modifies ligand‑induced signaling in multiple ways. For example, expression of endoglin can inhibit TGF‑ beta 1 signals but enhance BMP7 signals in the same myoblast cell line (11). In endothelial cells, endoglin inhibits T beta RI/ALK5, but enhances ALK1‑mediated activation (12). Deletion of mouse endoglin causes lethal vascular and cardiovascular defects, and human endoglin haploinsufficiency can a cause the vascular disorder, hereditary hemorrhagic telangiectasia type I (13, 14). These abnormalities confirm the essential function of endoglin in differentiation of smooth muscle, angiogenesis, and neovascularization (2‑4, 12‑14). In preeclampsia of pregnancy, high levels of proteolytically generated soluble endoglin and VEGF R1 (sFLT1), along with low placental growth factor (PlGF), are pathogenic due to antiangiogenic activity (15).

Item	Value
References	Gougos, A. and Letarte, M. (1990) J. Biol. Chem. 265:8361. ten Dijke, P. et al. (2008) Angiogenesis 11:79. Bernabeu, C. et al. (2007) J. Cell. Biochem. 102:1375. Mancini, M.L. et al. (2007) Dev. Biol. 308:520. Moody, J.L. et al. (2007) Stem Cells 25:2809. Velasco, S. et al. (2008) J. Cell Sci. 121:913. Perez-Gomez, E. et al. (2005) Oncogene 24:4450. Cheifetz, S, et al. (1992) J. Biol. Chem. 267:19027. Barbara, N.P. et al. (1999) J. Biol. Chem. 274:584. Scharpfenecker, M. et al. (2007) J. Cell Sci. 120:964. Scherner, O. et al. (2007) J. Biol. Chem. 282:13934. Pece-Barbara, N. et al. (2005) J. Biol. Chem. 280:27800. Arthur, H.M. et al. (2000) Dev. Biol. 217:42. Lebrin, F. and C.L. Mummery (2008) Trends Cardiovasc. Med. 18:25. Venkatesha, S. et al. (2006) Nat. Med. 12:642.
Entrez Gene IDs	2022 (Human); 13805 (Mouse); 497010 (Rat)
Alternate Names	CD105, CD105 antigen, ENDOsler-Rendu-Weber syndrome 1, ENG, Endoglin, HHT1FLJ41744, ORW, ORW1

Citations for Recombinant Human Endoglin/CD105 Protein (8)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human Endoglin/CD105 Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

E Gallardo-V et al. (2020), Potential Role of Circulating Endoglin in Hypertension via the Upregulated Expression of BMP4 Cells, 9(4).
PMID: 32316263
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

L Pérez-Roqu et al. (2020), Pregnancy-Induced High Plasma Levels of Soluble Endoglin in Mice Lead to Preeclampsia Symptoms and Placental Abnormalities International Journal of Molecular Sciences, 22(1).
PMID: 33375253
Species: Human
Sample Types: Protein
Applications: Bioassay

DG Walker et al. (2019), Microglial Phenotyping in Neurodegenerative Disease Brains: Identification of Reactive Microglia with an Antibody to Variant of CD105/Endoglin Cells, 8(7).
PMID: 31340569
Species: Human
Sample Types: Protein Array
Applications: Bioassay

E Gallardo-V et al. (2019), Endoglin Protein Interactome Profiling Identifies TRIM21 and Galectin-3 as New Binding Partners Cells, 8(9).
PMID: 31540324
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Ruiz-Remol et al. (2017), Circulating soluble endoglin modifies the inflammatory response in mice PLoS ONE, 12(11):e0188204.
PMID: 29145462
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Olsen O et al. (2014), Bone morphogenetic protein-9 suppresses growth of myeloma cells by signaling through ALK2 but is inhibited by endoglin. Blood Cancer J, 4(0):e196.
PMID: 24658374
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Rossi E et al. (2013), Endothelial endoglin is involved in inflammation: role in leukocyte adhesion and transmigration. Blood, 121(2):403-15.
PMID: 23074273
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hawinkels LJ et al. (2010), Matrix metalloproteinase-14 (MT1-MMP)-mediated endoglin shedding inhibits tumor angiogenesis. Cancer Res., 70(10):4141-50.
PMID: 20424116

Recombinant Human Endoglin/CD105 Protein

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Key Product Details

Complete Your Research

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Product Specifications

Formulation, Preparation and Storage

Carrier Free

1097-EN

Carrier

1097-EN/CF

Carrier Free

Reconstitution Calculator

Background: Endoglin/CD105

Citations for Recombinant Human Endoglin/CD105 Protein (8)

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