DAPT

Product Description

DAPT is a γ-secretase inhibitor. DAPT reduces Aβ40 and Aβ42 levels in human primary neuronal cultures (IC₅₀ values are 115 and 200 nM for total Aβ and Aβ42 respectively) and in brain extract, cerebrospinal fluid and plasma in vivo. DAPT has no effect on APPα and APPβ levels. DAPT blocks Notch signaling in hybrid human-mouse fetal thymus organ culture (FTOC) and causes ESCs to commit to neuronal differentiation. DAPT can be used in a small molecule cocktail to derive cortical and dopaminergic neurons from hPSCs (see our protocols below). Used in protocols to generate myoblasts from human human iPSCs. DAPT also promotes the formation of cone photoreceptors in retinal organoids.

For more information about how DAPT may be used, see our protocols: Accelerated Induction of Cortical Neurons from hiPSCs, Human iPSC-derived Myoblasts, Generating Midbrain Dopaminergic Neurons from hPSCs.

DAPT synthesized to Ancillary Material Grade also available.

Solubility

Preparing Stock Solutions for DAPT

The following data is based on the product molecular weight 432.46

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

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Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.31 mL	11.56 mL	23.12 mL
5 mM	0.46 mL	2.31 mL	4.62 mL
10 mM	0.23 mL	1.16 mL	2.31 mL
50 mM	0.05 mL	0.23 mL	0.46 mL

Calculators

Background References

References are publications that support the biological activity of the product.

• Xiang Long-term functional maintenance of primary human hepatocytes in vitro. Science 2019 PMID: 31023926
• Zerti Developing a simple method to enhance the generation of cone and rod photoreceptors in pluripotent stem cell-derived retinal organoids. Stem Cells 2020 PMID: 31670434
• Qi Combined small-molecule inhibition accelerates the derivation of functional cortical neurons from human pluripotent stem cells. Nat.Biotechnol. 2017 PMID: 28112759
• Sato Snapshot: growing organoids from stem cells. Cell 2015 PMID: 26091044
• Crawford and Roelink The notch response inhibitor DAPT enhances neuronal differentiation in embryonic stem cell-derived embryoid bodies independently of sonic hedgehog signaling. Dev.Dyn. 2007 PMID: 17295317
• De Smedt Different thresholds of notch signaling bias human precursor cells towards B-, NK-, monocytic/dendritic, or T-cell lineage in thymus microenvironment. Blood 2005 PMID: 16030192
• Dovey Functional gamma-secretase inhibitors reduce beta-amyloid peptide levels in brain. J.Neurochem. 2001 PMID: 11145990