Skip to main content

Novus Biologicals products are now on bio-techne.com

Better as one

Recombinant Human BDNF Protein

Bio-Techne includes R&D Systems | Catalog # 248-BDB

Catalog #
Availability
Size / Price
Qty
Loading...
Carrier Free
248-BDB-010/CF
248-BDB-01M/CF
Show More Sizes
248-BDB-050/CF
248-BDB-250/CF
Show Fewer Sizes

Catalog #
Availability
Size / Price
Qty
With Carrier
248-BDB-005
248-BDB-010
Show More Sizes
248-BDB-01M
248-BDB-050
248-BDB-250
Show Fewer Sizes

Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Conjugate

Unconjugated

Applications

Bioactivity, Binding Activity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived BDNF protein
His129-Arg247
Accession # P23560
100% sequence homology with Mouse, Rat, Canine, Equine and all other mammalian proteins examined.

Purity

>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

His129

Predicted Molecular Mass

13.5 kDa

SDS-PAGE

13-14 kDa, under reducing conditions.

Activity

Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB.
The ED50 for this effect is 0.2-2 ng/mL.

Measured by its binding ability in a functional ELISA.
When Recombinant Human TrkB Fc Chimera (Catalog # 688-TK) is coated at 1 μg/mL, Recombinant Human BDNF binds with an apparent Kd <1 nM.

Scientific Data Images for Recombinant Human BDNF Protein

Recombinant Human BDNF Protein Bioactivity Comparison.

As an alternative, please consider our next generation CHO-derived Recombinant Human BDNF (11166-BD). It has equivalent bioactivity to Sf 21 (baculovirus)-derived Recombinant Human BDNF (Catalog # 248-BDB). It combines R&D Systems quality with scalability that allows for a solid supply chain. Both Recombinant Human BDNF proteins are measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 248-BDB
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and NaCl with BSA as a carrier protein.
Reconstitution Reconstitute 5 µg vials at 100 µg/mL in water. Reconstitute 10 µg or larger vials at 250 µg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 248-BDB/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Sodium Citrate and NaCl.
Reconstitution Reconstitute at 250 μg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: BDNF

Brain-derived neurotrophic factor (BDNF) is a member of the NGF family of neurotrophic factors (also named neurotrophins) that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including NGF, BDNF, NT-3, and NT-4/5. These secreted cytokines are synthesized as prepropeptides that are proteolytically processed to generate the mature proteins (1, 2). All neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds and all share approximately 55% sequence identity at the amino acid level. Similarly to NGF, bioactive BDNF is predicted to be a non-covalently linked homodimer. BDNF cDNA encodes a 247 amino acid residue precursor protein with a signal peptide and a proprotein that are cleaved to yield the 119 amino acid residue mature BDNF. The amino acid sequence of mature BDNF is identical in all mammals examined. High levels of expression of BDNF have been detected in the hippocampus, cerebellum, fetal eye and placenta. In addition, low levels of BDNF expression are also found in the pituitary gland, spinal cord, heart, lung and skeletal muscle. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor (3). The BDNF signaling pathway utilizes both AKT and ERK pathways to exert its pleiotrophic effects in the central nervous system. BDNF is widely used in cell culture to support neuronal cell differentiation and survival. It also promotes differentiation of pluripotent stem cells into neural progenitor cells.

References

  1. Eide, F.F. et al. (1993) Exp. Neurol. 121:200.
  2. Snider, W.D. (1994) Cell 77:627.
  3. Barbacid, M. (1994) J. Neurobiol. 25:1386.

Long Name

Brain-derived Neurotrophic Factor

Alternate Names

Abrineurin, ANON2, BULN2, Neurotrophin

Entrez Gene IDs

627 (Human); 12064 (Mouse); 24225 (Rat)

Gene Symbol

BDNF

UniProt

Additional BDNF Products

Product Documents for Recombinant Human BDNF Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human BDNF Protein

For research use only

Citations for Recombinant Human BDNF Protein (230)

Showing  1 - 10 of 230 publications Showing All
Filter By:
Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human BDNF Protein include:

There are no citations that match your criteria.

Showing  1 - 10 of 230 publications Showing All

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

÷
=