Recombinant Human BDNF Protein, CF

Name: Recombinant Human BDNF Protein, CF
Brand: R&D Systems
SKU: 11166-BD
Price: 239 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 11166-BD

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	CHO
Accession #:	P23560.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human BDNF Protein, CF

Product Specifications for Recombinant Human BDNF Protein, CF

Source	Chinese Hamster Ovary cell line, CHO-derived human BDNF protein His129-Arg247
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	His129
Predicted Molecular Mass	13.5 kDa
SDS-PAGE	11-15 kDa, under reducing conditions.
Activity	Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED₅₀ for this effect is 0.200-2.00 ng/mL.

Scientific Data Examples for Recombinant Human BDNF Protein, CF

	Recombinant Human BDNF Protein Bioactivity. Measured in a cell proliferation assay using BaF mouse pro-B cells transfected with TrkB. The ED₅₀ for this effect is 0.200-2.00 ng/mL.
	Recombinant Human BDNF Protein SDS-PAGE. 2 μg/lane of Recombinant Human BDNF Protein (Catalog # 11166-BD) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 11-15 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

11166-BD

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 100-500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: BDNF

Brain-derived neurotrophic factor (BDNF) is a member of the neurotrophin family of growth factors that are required for the differentiation and survival of specific neuronal subpopulations in both the central as well as the peripheral nervous system. The neurotrophin family is comprised of at least four proteins including BDNF, nerve growth factor (NGF), neurotrophin-3 (NT3), and NT4/5. Human BDNF is initially expressed as a proprotein, which is then cleaved to yield a mature protein. Mature BDNF is a non-covalently linked homodimer, with each monomer containing antiparallel beta -strands and a characteristic cystine knot motif. Within the mature domain, human BDNF shares the identical amino acid sequence with mature mouse and rat BDNF. BDNF is strongly expressed in various regions of the brain, including the hippocampus and cerebellum, and weaker expression has been detected in the thymus, liver, spleen, heart, and lung. BDNF participates in axonal growth and pathfinding and in the modulation of dendritic growth and morphology and in later stages of development regulates synaptic transmission and plasticity and acts as a central modulator of pain. BDNF binds with high affinity and specifically activates the TrkB tyrosine kinase receptor. BDNF signaling via TrkB is essential for adult synaptic plasticity and the formation of memories. The BDNF signaling pathway utilizes both AKT and ERK pathways to exert its pleiotrophic effects in the central nervous system. Decreased expression of BDNF is seen in many neurological diseases such as Alzheimer's disease, Parkinson's disease, Huntington's disease, and autism. Further, BDNF is proposed as a biomarker for psychiatric disorders such as bipolar disease and depression and has been implicated in posttraumatic stress disorder, phobia, and panic disorder. A single amino acid substitution, Val66Met, has been shown to lead to reduced, activity-dependent BDNF secretion and memory impairment.

Item	Value
References	Numakawa, T. et al. (2010) Histol. Histopathol. 25:237. Egan, M.F. et al. (2003) Cell 112:257. Pruunsild, P. et al. (2007) Genomics 90:397. Bathina, S. and Das, U.N. (2015) AMS 11:1164. Binder, D.K. and Scharfman, H.E.(2004) Growth Factors 22:123. Cattaneo, A. et al. (2016) Transl. Psychiatry 6:e958. Andero, R. et al. (2011) Am J. Psychiatry 168:163.
Long Name	Brain-derived Neurotrophic Factor
Entrez Gene IDs	627 (Human); 12064 (Mouse); 24225 (Rat)
Alternate Names	ANON2, Abrineurin, BDNF, BULN2, MGC34632, Neurotrophin, brain-derived neurotrophic factor

Citations for Recombinant Human BDNF Protein, CF

Please visit our Instrument Citation Database to search our collection of scientific articles that feature our instruments.

Recombinant Human BDNF Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Product Specifications for Recombinant Human BDNF Protein, CF

Scientific Data Examples for Recombinant Human BDNF Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: BDNF

Citations for Recombinant Human BDNF Protein, CF

Product Documents for Recombinant Human BDNF Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

Bio-Techne Brands

Stay Connected