Recombinant Human TrkB Fc Chimera Protein, CF

Name: Recombinant Human TrkB Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 688-TK
Rating: 5 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 688-TK

Citations (51)

(3)

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Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	Q16620.1
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Human TrkB Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived human TrkB protein

HumanTrkB (Cys32-His430) Accession # Q16620.1	DIEGRMD	Human IgG₁ (Pro100-Lys330)	6-His tag
N-terminus			C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Cys32

Predicted Molecular Mass

71.7 kDa (monomer)

SDS-PAGE

120 kDa-130 kDa, reducing conditions

Activity

Measured by its ability to inhibit BDNF-induced proliferation of BaF‑TrkB‑BD mouse pro‑B cells transfected with TrkB. The ED₅₀ for this effect is 0.1‑0.4 µg/mL in the presence of 16 ng/mL of recombinant human BDNF.

Scientific Data Examples for Recombinant Human TrkB Fc Chimera Protein, CF

	Recombinant Human TrkB Fc Chimera Protein Bioactivity Recombinant Human TrkB Fc Chimera (Catalog # 688-TK) inhibits BDNF-induced cell proliferation of the BaF‑TrkB‑BD mouse pro-B cell line transfected with TrkB. The ED₅₀ for this effect is 0.1-0.4 μg/mL in the presence of 16 ng/mL of recombinant human BDNF.
	Recombinant Human TrkB Fc Chimera Protein SDS-PAGE 1 μg/lane of Recombinant Human TrkB Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing single bands at 120 kDa and 200 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

688-TK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TrkB

The neurotrophins, including NGF, BDNF, NT-3, and NT-4/5 constitute a group of structurally related, secreted proteins that play an important role in the development and function of the nervous system. The biological activities of the neurotrophins are mediated by binding to the different members of the Trk family tyrosine kinase receptors. Three Trk family proteins, TrkA, TrkB, and TrkC, exhibiting different ligand specificities, have been identified. TrkA binds NGF, TrkB binds BDNF and NT‑4/5 and TrkC binds NT-3. All Trk family proteins share a conserved complex subdomain organization consisting of a signal peptide, two cysteine-rich domains, a cluster of three leucine-rich motifs, and two immunoglobulin-like domains in the extracellular region, as well as an intracellular region that contains the tyrosine kinase domain. Natural splice variants of the different Trks, including TrkB variants lacking the first cysteine-rich domain, the first and second or all three of the leucine-rich motifs, or the tyrosine kinase domain, have been described. The role of the different extracellular subdomains of TrkB in mediating neurotrophin binding and discrimination is currently being investigated. At the protein sequence level, human and rat TrkB are greater than 90% identical and the proteins exhibit cross-species activity. TrkB is primarily expressed in the nervous system. However, low levels of TrkB expression have also been observed in a wide variety of tissues (pancreas, kidneys, ovary) outside the nervous system.

Item	Value
References	Ninkina, N. et al. (1997) J. Biol. Chem. 272:13019. Middlemas, D.S. et al. (1991) Mol. Cell Biol. 11:143. Soppet, D. et al. (1991) Cell 65:895.
Long Name	Neurotrophic Tyrosine Kinase Receptor B
Entrez Gene IDs	4915 (Human); 18212 (Mouse); 25054 (Rat)
Alternate Names	BDNF/NT-3 growth factors receptor, EC 2.7.10, EC 2.7.10.1, GP145-TrkB, NTRK2, Neurotrophic tyrosine kinase receptor type 2, TRKBBDNF-tropomyosine receptor kinase B, TrkB, TrkB tyrosine kinase, neurotrophic tyrosine kinase, receptor, type 2, trk-B, tyrosine kinase receptor B

Citations for Recombinant Human TrkB Fc Chimera Protein, CF (51)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human TrkB Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: In Vivo
Applications: Bioassay

MV Pavon et al. (2022), Inhibition of Nogo-A rescues synaptic plasticity and associativity in APP/PS1 animal model of Alzheimer's disease Seminars in cell developmental biology, 2022;0(0).
PMID: 35431138
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

J Zhou et al. (2022), NMDA receptor-dependent prostaglandin-endoperoxide synthase 2 induction in neurons promotes glial proliferation during brain development and injury Cell Reports, 38(13):110557.
PMID: 35354047
Species: Mouse
Sample Types: In Vivo
Applications: Bioassay

Y Lin et al. (2021), Environmental enrichment implies GAT-1 as a potential therapeutic target for stroke recovery Theranostics, 11(8):3760-3780.
PMID: 33664860
Species: Human
Sample Types: Whole Cells
Applications: Neutralization

Y Zhang et al. (2021), Quantitative description of the interactions among kinase cascades underlying long-term plasticity of Aplysia sensory neurons Scientific Reports, 11(1):14931.
PMID: 34294802
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

PM Horvath et al. (2021), A subthreshold synaptic mechanism regulating BDNF expression and resting synaptic strength Cell Reports, 36(5):109467.
PMID: 34348149
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

A Petkova-Tu et al. (2021), Neuroligin-1 mediates presynaptic maturation through brain-derived neurotrophic factor signaling Bmc Biology, 19(1):215.
PMID: 34579720
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

X Li et al. (2020), Exercise training modulates glutamic acid decarboxylase-65/67 expression through TrkB signaling to ameliorate neuropathic pain in rats with spinal cord injury Mol Pain, 16(0):1744806920924.
PMID: 32418502
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

A Patnaik et al. (2020), Fingolimod Modulates Dendritic Architecture in a BDNF-Dependent Manner Int J Mol Sci, 21(9).
PMID: 32349283
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

G Fulgenzi et al. (2020), Novel metabolic role for BDNF in pancreatic &beta-cell insulin secretion Nat Commun, 11(1):1950.
PMID: 32327658
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

AG González et al. (2020), The Rab5-Rab11 endosomal pathway is required for BDNF-induced CREB transcriptional regulation in hippocampal neurons J. Neurosci., 0(0).
PMID: 32928890
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

LE Montroull et al. (2019), Loss of TrkB Signaling Due to Status Epilepticus Induces a proBDNF-Dependent Cell Death Front Cell Neurosci, 13(0):4.
PMID: 30800056
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

X Li et al. (2018), Blocking of BDNF-TrkB signaling inhibits the promotion effect of neurological function recovery after treadmill training in rats with spinal cord injury Spinal Cord, 0(0).
PMID: 30002472
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Q Wu et al. (2018), Human menstrual blood-derived stem cells promote functional recovery in a rat spinal cord hemisection model Cell Death Dis, 9(9):882.
PMID: 30158539
Species: Rat
Sample Types: In Vivo
Applications: In vivo

JM Hall et al. (2018), Nerve Growth Factor Is Responsible for Exercise-Induced Recovery of Septohippocampal Cholinergic Structure and Function Front Neurosci, 12(0):773.
PMID: 30443202
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

H Zhang et al. (2018), Modulation of AMPA receptor surface diffusion restores hippocampal plasticity and memory in Huntington's disease models Nat Commun, 9(1):4272.
PMID: 30323233
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

CRAF Diniz et al. (2018), Antidepressant-like effect of losartan involves TRKB transactivation from angiotensin receptor type 2 (AGTR2) and recruitment of FYN Neuropharmacology, 135(0):163-171.
PMID: 29550391
Species: N/A
Sample Types: Protein
Applications: Bioassay

M Sharma et al. (2017), Epigenetic regulation by G9a/GLP complex ameliorates amyloid-beta 1-42 induced deficits in long-term plasticity and synaptic tagging/capture in hippocampal pyramidal neurons Aging Cell, 0(0).
PMID: 28665013
Species: Human
Sample Types: Protein
Applications: Bioassay

A Suzuki et al. (2017), Inhibitory Effects of Polyacetylene Compounds from Panax ginseng on Neurotrophin Receptor-Mediated Hair Growth Biol. Pharm. Bull., 40(10):1784-1788.
PMID: 28966252
Species: N/A
Sample Types: Protein
Applications: Bioassay

JN Sleigh et al. (2017), Trk receptor signaling and sensory neuron fate are perturbed in human neuropathy caused by Gars mutations Proc. Natl. Acad. Sci. U.S.A, 0(0).
PMID: 28351971
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

LF Martins et al. (2017), Mesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF Sci Rep, 7(1):4153.
PMID: 28646200
Species: Rat
Sample Types: Cell Culture Supernates
Applications: Bioassay

Y Guo et al. (2017), BDNF/NT3 Regulate AIS Location and Affect Neuronal Excitability in Cultured Hippocampal Neurons J. Neurochem., 0(0).
PMID: 28440877
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

JI Hurtado-Za et al. (2017), TRPV1 regulates excitatory innervation of OLM neurons in the hippocampus Nat Commun, 8(0):15878.
PMID: 28722015
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

RR Nair et al. (2017), Dynamic Arc SUMOylation and Selective Interaction with F-Actin-Binding Protein Drebrin A in LTP Consolidation In Vivo Front Synaptic Neurosci, 9(0):8.
PMID: 28553222
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

(2016), Potentiation of Synaptic GluN2B NMDAR Currents by Fyn Kinase Is Gated through BDNF-Mediated Disinhibition in Spinal Pain Processing Cell Rep, 17(10):2753-2765.
PMID: 27926876
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

James W Grau (2016), Complete spinal cord injury (SCI) transforms how brain derived neurotrophic factor (BDNF) affects nociceptive sensitization Exp. Neurol., 288(0):38-50.
PMID: 27818188
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Yoshinori Hayashi (2016), BK channels in microglia are required for morphine-induced hyperalgesia Nat Commun, 7(0):11697.
PMID: 27241733
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Sorge R et al. (2015), Different immune cells mediate mechanical pain hypersensitivity in male and female mice. Nat Neurosci, 18(8):1081-3.
PMID: 26120961
Species: Rat
Sample Types: Whole Tissue
Applications: Bioassay

Obis T et al. (2015), The novel protein kinase C epsilon isoform at the adult neuromuscular synapse: location, regulation by synaptic activity-dependent muscle contraction through TrkB signaling and coupling to ACh release. Mol Brain, 8(0):8.
PMID: 25761522
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Zhu G et al. (2015), Different patterns of electrical activity lead to long-term potentiation by activating different intracellular pathways. J Neurosci, 35(2):621-33.
PMID: 25589756
Species: Rat
Sample Types: Whole Tissue
Applications: Bioassay

Rittase et al. (2014), Dynorphin up-regulation in the dentate granule cell mossy fiber pathway following chronic inhibition of GluN2B-containing NMDAR is associated with increased CREB (Ser 133) phosphorylation, but is independent of BDNF/TrkB signaling pathways. Mol Cell Neurosci, 60(0):63-71.
PMID: 24769103
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Bambah-Mukku D et al. (2014), A positive autoregulatory BDNF feedback loop via C/EBPbeta mediates hippocampal memory consolidation. J Neurosci, 34(37):12547-59.
PMID: 25209292
Species: Rat
Sample Types: Cell Culture Supernates
Applications: Bioassay

Vermehren-Schmaedick et al. (2014), Heterogeneous intracellular trafficking dynamics of brain-derived neurotrophic factor complexes in the neuronal soma revealed by single quantum dot tracking. PLoS ONE, 9(4):e95113.
PMID: 24732948
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Bao Y et al. (2014), PAR2-mediated upregulation of BDNF contributes to central sensitization in bone cancer pain. Mol Pain, 10(0):28.
PMID: 24886294
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Melemedjian O et al. (2013), BDNF regulates atypical PKC at spinal synapses to initiate and maintain a centralized chronic pain state. Mol Pain, 9(0):12.
PMID: 23510079
Species: Rat
Sample Types: In Vivo
Applications: Bioassay

Chen D et al. (2012), Glucocorticoid receptors recruit the CaMKIIalpha-BDNF-CREB pathways to mediate memory consolidation. Nat Neurosci, 15(12):1707-14.
PMID: 23160045
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Tsai CY et al. (2012), Brain-derived neurotrophic factor ameliorates brain stem cardiovascular dysregulation during experimental temporal lobe status epilepticus. PLoS ONE, 7(3):e33527.
PMID: 22442695
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Makino et al. (2012), Inhibition of uterine sarcoma cell growth through suppression of endogenous tyrosine kinase B signaling. PLoS ONE, 7(7):e41049.
PMID: 22911740
Species: N/A
Sample Types: Protein
Applications: Surface Plasmon Resonance

Vaegter CB et al. (2011), Sortilin associates with Trk receptors to enhance anterograde transport and neurotrophin signaling. Nat. Neurosci., 14(1):54-61.
PMID: 21102451
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Renn CL et al. (2011), Brain-derived neurotrophic factor modulates antiretroviral-induced mechanical allodynia in the mouse. J. Neurosci. Res., 89(10):1551-65.
PMID: 21647939
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Huang YT et al. (2010), BDNF mediated TrkB activation is a survival signal for transitional cell carcinoma cells. Int. J. Oncol., 36(6):1469-76.
PMID: 20428771
Species: Human
Sample Types: Recombinant Protein
Applications: Surface Plasmon Resonance

Fobian K et al. (2010), Peptides derived from the solvent-exposed loops 3 and 4 of BDNF bind TrkB and p75(NTR) receptors and stimulate neurite outgrowth and survival. J. Neurosci. Res., 88(6):1170-81.
PMID: 19908279
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Endo T et al. (2009), Early exercise in spinal cord injured rats induces allodynia through TrkB signaling. Biochem. Biophys. Res. Commun., 381(3):339-44.
PMID: 19222991
Fitzgerald M et al. (2008), Neurite responses to ephrin-A5 modulated by BDNF: evidence for TrkB-EphA interactions. Biochem. Biophys. Res. Commun., 374(4):625-30.
PMID: 18674513
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Gomez-Pinilla F et al. (2008), Brain-derived neurotrophic factor functions as a metabotrophin to mediate the effects of exercise on cognition. Eur. J. Neurosci., 28(11):2278-87.
PMID: 19046371
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Ying Z et al. (2008), BDNF-exercise interactions in the recovery of symmetrical stepping after a cervical hemisection in rats. Neuroscience, 155(4):1070-8.
PMID: 18672032
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Xapelli S et al. (2008), Interaction between neuropeptide Y (NPY) and brain-derived neurotrophic factor in NPY-mediated neuroprotection against excitotoxicity: a role for microglia. Eur. J. Neurosci., 27(8):2089-102.
PMID: 18412629
Species: Bacteria
Sample Types: Trypanosoma
Applications: PcIP

Lu B et al. (2008), A novel immunoprecipitation strategy identifies a unique functional mimic of the glial cell line-derived neurotrophic factor family ligands in the pathogen Trypanosoma cruzi. Infect. Immun., 76(8):3530-8.
PMID: 18541656
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Chytrova G et al. (2007), Exercise normalizes levels of MAG and Nogo-A growth inhibitors after brain trauma. Eur. J. Neurosci., 27(1):1-11.
PMID: 18093178
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Gomez-Pinilla F et al. (2007), BDNF and learning: Evidence that instrumental training promotes learning within the spinal cord by up-regulating BDNF expression. Neuroscience, 148(4):893-906.
PMID: 17719180
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Vaynman SS et al. (2006), Exercise differentially regulates synaptic proteins associated to the function of BDNF. Brain Res., 1070(1):124-30.
PMID: 16413508
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay

Esper RM et al. (2004), Rapid axoglial signaling mediated by neuregulin and neurotrophic factors. J. Neurosci., 24(27):6218-27.
PMID: 15240814

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Customer Reviews for Recombinant Human TrkB Fc Chimera Protein, CF (3)

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4 stars

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Recombinant Human TrkB Fc Chimera Protein, CF

Name: Anonymous

Application: In vitro bioactivity in cell culture

Verified Customer | Posted 09/07/2018

Rat cortical neurons treated with 4ug/ml TrkB at 37C for 30min then with 5ng/ml Bdnf for 1h, and harvested for RT-PCR with primers specific for cFos.
Recombinant Human TrkB Fc Chimera Protein, CF

Name: Anonymous

Application: In vivo study

Verified Customer | Posted 07/18/2016
Recombinant Human TrkB Fc Chimera Protein, CF

Name: Juan Zanin

Application: Apoptosis assay

Reason for Rating: it blocks the activation of TrkB via BDNF.

Verified Customer | Posted 05/18/2016

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Product Documents for Recombinant Human TrkB Fc Chimera Protein, CF

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Recombinant Human TrkB Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

Complete Your Experiment

Product Specifications for Recombinant Human TrkB Fc Chimera Protein, CF

Scientific Data Examples for Recombinant Human TrkB Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: TrkB

Citations for Recombinant Human TrkB Fc Chimera Protein, CF (51)

Customer Reviews for Recombinant Human TrkB Fc Chimera Protein, CF (3)

Customer Images

Recombinant Human TrkB Fc Chimera Protein, CF

Recombinant Human TrkB Fc Chimera Protein, CF

Recombinant Human TrkB Fc Chimera Protein, CF

Product Documents for Recombinant Human TrkB Fc Chimera Protein, CF

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