Purmorphamine
Tocris Bioscience, part of Bio-Techne | Catalog # 4551
Smo receptor agonist
Product Description
Purmorphamine is a smoothened (Smo) receptor agonist (EC50 ~ 1 μM) and activates the Hedgehog pathway. Purmorphamine induces osteogenesis in mouse mesenchymal progenitor cells (C3H10T1/2). When combined with BMP-4, Purmorphamine can transdifferentiate pre-adipocytes (3T3-L1) and myoblasts (C2C12) into osteoblasts. Purmorphamine induces differentiation of multipotent mesenchymal progenitor cells into osteoblasts, and of spinal motor neurons from pluripotent human stem cells, and protects dopaminergic neurons in a mouse model of Parkinson's disease.Scientific Data Images for Purmorphamine
Applications of Purmorphamine in Neurons Derived from hiPSCs
Dopaminergic neurons derived from human induced pluripotent stem cells (hiPSC) using SB 431542 (Catalog # 1614, Tocris), CHIR 99021 (Catalog # 4423, Tocris), DAPT (Catalog # 2634, Tocris) and Purmorphamine. Cells cultured in Neuronal Media supplemented with TGF-3 (Catalog # 8420-B3, R&D Systems), cAMP, GDNF (Catalog # 212-GD, R&D Systems), BDNF (Catalog # 248-BDB, R&D Systems), N21-MAX (Catalog # AR008, R&D Systems). Differentiation shown at days 55 (left) and 62 (right). Images courtesy of Kevin Flynn, Bio-Techne.Product Specifications
Molecular Weight
520.62
Formula
C31H32N6O2
Storage
Store at -20°C
Purity
≥98% (HPLC)
Chemical Name
9-Cyclohexyl-N-[4-(4-morpholinyl)phenyl]-2-(1-naphthalenyloxy)-9H-purin-6-amine
CAS Number
483367-10-8
PubChem ID
5284329
InChI Key
FYBHCRQFSFYWPY-UHFFFAOYSA-N
SMILES
C1(OC4=CC=CC5=C4C=CC=C5)=NC(NC3=CC=C(N6CCOCC6)C=C3)=C2C(N(C7CCCCC7)C=N2)=N1
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solvent | Max Conc. mg/mL | Max Conc. mM | |
|---|---|---|---|
| Solubility | |||
| DMSO | 52.06 | 100 |
Preparing Stock Solutions
for Purmorphamine
The following data is based on the product molecular weight 520.62
Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which all affect the solvent volumes required to prepare stock solutions.
| Concentration / Solvent Volume / Mass | 1 mg | 5 mg | 10 mg |
|---|---|---|---|
| 1 mM | 1.92 mL | 9.60 mL | 19.21 mL |
| 5 mM | 0.38 mL | 1.92 mL | 3.84 mL |
| 10 mM | 0.19 mL | 0.96 mL | 1.92 mL |
| 50 mM | 0.04 mL | 0.19 mL | 0.38 mL |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Background References
References are publications that support the biological activity of the product.
- Sinha Purmorphamine activates the Hedgehog pathway by targeting Smoothened. Nat.Chem.Biol. 2006 PMID: 16408088
- Wu A small molecule with osteogenesis-inducing activity in multipotent mesenchymal progenitor cells. J.Am.Chem.Soc. 2002 PMID: 12465946
- Wu Purmorphamine induces osteogenesis by activation of the hedgehog signaling pathway. Chem.Biol. 2004 PMID: 15380183
Product Specific Notices for Purmorphamine
For research use only
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