Immuno-Oncology: Detection & Analysis

Single-Cell Analysis

Quantitative single-cell measurement techniques are essential for gaining valuable insight into the underlying mechanisms of heterogeneity. Our solutions range from transcriptomic to cytometric, and even overcome the hurdles single-cell proteomic approaches have faced to date!

Cytometric and Proteomic:

• Build your next Flow panel with a wide selection of specific marker antibodies that can be conjugated to an assortment of fluorescent dyes
• Determine the frequency of cytokine secretion for a single cell with the most accurate and sensitive ready-to-go ELISpot Kits available
• Characterize FACS-sorted, highly enriched, low abundance, cell populations with Single-Cell Westerns on Milo™

Transcriptomic and Proteomic:

• Acquire the spatial signature of single-cell transcriptomes in tissue using the RNAscope™ and BaseScope™ assays 
• Validate single-cell RNA sequencing data using the RNAscope™ Technology, followed by protein level analysis via Single-Cell Westerns on Milo and conventional Western blot antibodies

Single and Multiplex Immunoassays

We created Quantikine^® ELISAs, the most trusted and cited on the market. Our range has expanded to accommodate all your immunoassay needs, delivering reliable results the first time and every time.

• Detect changes in up to 119 analytes in one sample using Proteome Profiler™ Antibody Arrays, then analyze results using FluorChem™ Imagers and AlphaView^® Software
• Multiplex up to 50 analytes using bead-based Luminex^® Assays
• Measure single- or multi-analyte levels in just 1 hour using highly sensitive Simple Plex™ assays on Ella that have a 3 to 5 log dynamic range and single-digit inter- and intra-assay CV values 
• Use the industry gold-standard, Quantikine^® ELISA Kits for single-analyte confirmation 
• Choose from over 800 flexible, single-analyte DuoSet^® ELISA Development Systems

Gene Expression

Verify gene expression within intact tissue, choose from a library of gene knockout-validated products, or dig further into the scientific literature to uncover an unbiased review of your scientific interests.

• Advance your biomarker discovery program with proprietary exosome-based technology to extract exosomal RNA from serum, plasma, and urine samples
• Use the RNAscope™ and BaseScope™ assays to detect and quantify RNA molecules at single-cell resolution
• Utilize the BaseScope™ assay for detection of short targets, highly homologous sequences, and splice variants
• Use Single-Cell Westerns on Milo to verify hypotheses based on single-cell RNA expression data  
• Shop gene knockout-validated antibodies and custom cell lines and cell lysates generated using CRISPR/Cas9 
• Validate CRISPR gene-edited cells with Jess’ automated capillary electrophoresis immunoassays
• Use Laverne, a bioinformatics tool, to facilitate scientific exploration of related genes, disease states and types, and pathways based on co-citations

Protein Expression

Look no further, our innovative fully automated platforms are versatile, efficient and quantitative; they’re sure to conquer all your protein analysis frustrations.

• Simple Western™ Assays on Jess™ and Wes™
  • Use just 3 µL of sample for picogram-level sensitivity 
  • Get quantitative data on up to 25 samples in just 3 hours 
  • Automated data analysis using Compass for Simple Western Software
  • Supported for use in QC and GMP environments
  • Thousands of validated antibodies available
  • Simple Western high-throughput assay platforms for size- and/or charge-based separation: Sally Sue™, Peggy Sue™, and NanoPro™ 1000
• Single-Cell Westerns on Milo
  • World’s first Single-Cell Western platform 
  • Measure protein expression heterogeneity in ~1,000 single cells in 4 hours
  • Automated and quantitative data analysis using Scout software 
  • Validated antibodies available
• FluorChem™ Systems for traditional Western blot imaging:
  • 5-log dynamic range lets you detect faint and bright bands at once
  • Complete analysis with AlphaView^® Software