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IL-6 Family Cytokines

Cell Proliferation Induced by R&D Systems Recombinant Human IL-6 and Neutralization by a Mouse Anti-Human IL-6 Monoclonal Antibody

Analysis of IL-6-induced proliferation by mouse plasmacytoma cells and neutralization by anti-human IL-6 monoclonal antibody

IL-6-induced Cell Proliferation is Neutralized Using a Mouse Anti-Human IL-6 Monoclonal Antibody. The T1165.85.2.1 mouse plasmacytoma cell line was treated with increasing concentrations of Recombinant Human IL-6 (R&D Systems, Catalog # 206-IL) and cell proliferation was assessed (orange line). The ED50 for this effect is 0.2-0.8 ng/mL. Proliferation stimulated by 2.5 ng/mL Recombinant Human IL-6 was neutralized by treating the cells with increasing concentrations of a Mouse Anti-Human/Primate IL-6 Monoclonal Antibody (R&D Systems, Catalog # MAB206R; blue line). The ND50 for this effect is typically 0.05 - 0.15 ug/mL.

Detection of gp130 in CD3+ Mouse Splenocytes by Flow Cytometry

Detection of gp130 in mouse splenocytes using a PE-conjugated rat anti-mouse gp130 monoclonal antibody and flow cytometry

Detection of gp130 in CD3+ Mouse Splenocytes by Flow Cyotmetry. Mouse splenocytes were stained with an APC-conjugated Rat Anti-Mouse CD3 Monoclonal Antibody (R&D Systems, Catalog # FAB4841A) and either a (A) PE-conjugated Rat Anti-Mouse gp130 Monoclonal Antibody (R&D Systems, Catalog # FAB4681A) or a (B) PE-conjugated Rat IgG2A Isotype Control Antibody (R&D Systems, Catalog # IC006P).

Cell Proliferation Induced by R&D Systems Recombinant Human IL-11 and Neutralization by a Mouse Anti-Human IL-11 Monoclonal Antibody

Analysis of IL-11-induced proliferation by mouse plasmacytoma cells and neutralization by anti-human IL-11 monoclonal antibody

IL-11-induced Cell Proliferation is Neutralized Using a Mouse Anti-Human IL-11 Monoclonal Antibody. The T11 mouse plasmacytoma cell line was treated with increasing concentrations of Recombinant Human IL-11 (R&D Systems, Catalog # 218-IL) and cell proliferation was assessed (orange line). The ED50 for this effect is 0.02-0.12 ng/mL. Proliferation stimulated by 1 ng/mL Recombinant Human IL-11 was neutralized by treating the cells with increasing concentrations of a Mouse Anti-Human IL-11 Monoclonal Antibody (R&D Systems, Catalog # MAB218; blue line). The ND50 for this effect is typically < 8 ug/mL.

Detection of IL-31RA in IFN-gamma-treated CD14+ Human PBMCs by Flow Cytometry

Detection of IL-31RA in IFN-gamma-treated CD14+ human PBMCs using a rat anti-human IL-31RA monoclonal antibody and flow cytometry

Detection of IL-31RA in IFN-gamma-treated Human PBMCs by Flow Cyotmetry. Human peripheral blood mononuclear cells (PBMCs) were (A) treated with 50 ng/mL Recombinant Human IFN-gamma (R&D Systems, Catalog # 285-IF) for 20 hours or (B) untreated, and then stained with an APC-conjugated Mouse Anti-Human CD14+ Monoclonal Antibody (R&D Systems, Catalog # FAB3832A) and a Rat Anti-Human IL-31 RA Monoclonal Antibody (R&D Systems, Catalog # MAB2769) followed by a PE-conjugated Goat Anti-Rat IgG Secondary Antibody (R&D Systems, Catalog # F0105B). Quadrant markers were set based on staining with a Rat IgG1 Isotype Control (R&D Systems, Catalog # MAB005).

Cell Proliferation Induced by R&D Systems Recombinant Human LIF and Neutralization by a Goat Anti-Human LIF R alpha Polyclonal Antibody

Analysis of LIF-induced proliferation by human erythroleukemic cells and neutralization by an anti-human LIF R alpha antibody

LIF-induced Cell Proliferation is Neutralized Using a Goat Anti-Human LIF R alpha Polyclonal Antibody. The TF-1 human erythroleukemic cell line was treated with increasing concentrations of Recombinant Human LIF (R&D Systems, Catalog # 7734-LF) and cell proliferation was assessed (orange line). The ED50 for this effect is 0.02-0.12 ng/mL. Proliferation stimulated by 0.3 ng/mL Recombinant Human LIF was neutralized by treating the cells with increasing concentrations of a Goat Anti-Human LIF R alpha Affinity-purified Polyclonal Antibody (R&D Systems, Catalog # AF-249-NA; blue line). The ND50 for this effect is typically 6-36 ug/mL.

Cell Proliferation Induced by R&D Systems Recombinant Mouse Oncostatin M and Neutralization by a Rat Anti-Mouse Oncostatin M Monoclonal Antibody

Analysis of OSM-induced proliferation of mouse embryonic fibroblast cells and neutralization by an anti-mouse OSM antibody

Oncostatin M-induced Cell Proliferation is Neutralized Using a Rat Anti-Mouse Oncostatin M Monoclonal Antibody. The NIH-3T3 mouse embryonic fibroblast cell line was treated with increasing concentrations of Recombinant Mouse Oncostatin M (R&D Systems, Catalog # 495-MO) and cell proliferation was assessed (orange line). The ED50 for this effect is 0.25-1 ng/mL. Proliferation stimulated by 1 ng/mL Recombinant Mouse Oncostatin M was neutralized by treating the cells with increasing concentrations of a Rat Anti-Mouse Oncostatin M Monoclonal Antibody (R&D Systems, Catalog # MAB4951; blue line). The ND50 for this effect is typically 0.2-1.2 ug/mL.

Immunostaining of CNTF in Perfusion-fixed Frozen Sections of Rat Cerebellum Using R&D Systems Goat Anti-Rat CNTF Polyclonal Antibody

Immunostaining of CNTF in perfusion-fixed frozen sections of rat cerebellum using a Goat Anti-Rat CNTF Polyclonal Antibody

Detection of CNTF in Rat Cerebellum. Perfusion-fixed frozen sections of rat cerebellum were stained for CNTF using a Goat Anti-Mouse/Rat CNTF Antigen Affinity-purified Polyclonal Antibody (R&D Systems, Catalog # AF-557-NA) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific CNTF staining was localized to the Purkinje neurons.

Featured Products for IL-6 Family Research

image of a ProDot protein, a recombinant protein packaged into an easy-to-use ball, in a vial

ProDots™ Proteins

Ensure optimal protein recovery and reduce media preparation time with our single-use vials of lyophilized ProDots Proteins. ProDots Proteins are R&D SystemsTM proteins packaged into easy-to-use balls that can be rolled into your cell culture media. This product will not only simplify your media preparation by eliminating aliquoting and freeze/thaw cycles, but it also ensures 100% protein recovery.

Sandwich ELISA assay principle showing an antigen bound between specific capture and detection antibodies

Immunoassays for IL-6 Family Cytokine Detection

From our complete, ready-to-use Quantikine ELISA Kits to our more flexible DuoSet ELISA Development Systems, we offer a wide selection of immunoassays for measuring IL-6 family cytokines. Whatever your needs, you can count on our immunoassays to deliver accurate, reproducible, high-quality data for every experimental sample that you test.

Ribbon structure of a recombinant protein

Custom Protein Libraries

Are you spending valuable research time aliquoting proteins into plates? Let us help. We offer Custom Protein Libraries, which come with the proteins that you select already aliquoted into the wells of a 96-well plate. This custom product allows you to select the proteins that you need from a portfolio of over 5,000 R&D Systems bioactive proteins and will significantly reduce the amount of time needed to set-up your experiments.

Featured IL-6 Family Resources

Thumbnail cover of Bio-Techne’s Cytokines and Proteins User’s Guide eBook

Cytokines and Proteins User’s Guide eBook

Download this guide to learn about the different applications for cytokines in cell biology research. From cell culture and lineage differentiation to antibody and immunoassay development, this guide highlights how recombinant proteins are being utilized in diverse research areas.

Periodic Table of Human Cytokine and Chemokine Poster Teaser Solo

Periodic Table of Cytokine & Chemokine Families Wall Poster

Learn about the members of different cytokine and chemokine families with our periodic table wall poster. This poster includes information about the structures, receptors, and native molecular weights of each cytokine, and will serve as a great reference tool and colorful addition to your lab space.

IL-6 belongs to a family of long-chain four alpha helix bundle cytokines that bind to gp130 and mediate a range of effects

Cytokine Signaling Pathways

Cytokines activate a diverse array of intracellular signaling pathways that can induce processes such as cell proliferation, differentiation, migration, and inflammation. Explore the signaling pathways that are activated by different cytokine families, the primary target cells that they affect, and the biological effects that they mediate using our interactive signaling pathways.