Mouse/Rat CNTF Antibody

Name: Mouse/Rat CNTF Antibody
Brand: R&D Systems
SKU: AF-557-NA

R&D Systems, Inc. a Bio-Techne Brand

Catalog # AF-557-NA

Citations (5)

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Species Reactivity:	Mouse, Rat
Immunogen:	E. coli-derived recombinant rat CNTF Ala2-Met200 Accession # P20294.1
Applications:	Block/Neutralize, Immunohistochemistry
Label:	Unconjugated
Source:	Polyclonal Goat IgG
Specificity:	Detects rat CNTF in direct ELISAs and Western blots. Detects mouse and rat CNTF in immunohistochemistry. In direct ELISAs and Western blots, less than 15% cross-reactivity with recombinant human CNTF is observed.

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Applications for Mouse/Rat CNTF Antibody

	Recommended Concentration	Sample
Immunohistochemistry	5-15 µg/mL	See below

Item	Value
Neutralization	Measured by its ability to neutralize CNTF-induced proliferation in the TF‑1 human erythroleukemic cell line. The Neutralization Dose (ND₅₀) is typically 0.3-2.4 µg/mL in the presence of 20 ng/mL Recombinant Rat CNTF.

Please Note: Optimal dilutions should be determined by each laboratory for each application.

Scientific Data Examples for Mouse/Rat CNTF Antibody

	Immunohistochemistry CNTF in Mouse Cerebellum. CNTF was detected in perfusion fixed frozen sections of mouse cerebellum using Goat Anti-Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA) at 1.7 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
	Immunohistochemistry CNTF in Rat Cerebellum. CNTF was detected in perfusion fixed frozen sections of rat cerebellum using Goat Anti-Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
	Immunohistochemistry CNTF in Mouse Brain. CNTF was detected in perfusion fixed paraffin-embedded sections of mouse brain (hippocampus) using Goat Anti-Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes and cytoplasm of Purkinje cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
	Neutralization Cell Proliferation Induced by CNTF and Neutralization by Rat CNTF Antibody. Recom-binant Rat CNTF (Catalog # 557-NT) stimulates proliferation in the TF-1 human erythro-leukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat CNTF (20 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat CNTF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-557-NA). The ND₅₀ is typ-ically 0.3-2.4 µg/mL.

Formulation, Preparation and Storage

Purification	Antigen Affinity-purified
Endotoxin level	<0.10 EU per 1 μg of the antibody by the LAL method.
Reconstitution	Reconstitute at 0.2 mg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CNTF

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons, and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy. The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.

Item	Value
Long Name	Ciliary Neurotrophic Factor
Entrez Gene IDs	1270 (Human); 12803 (Mouse); 25707 (Rat)
Alternate Names	CNTF, HCNTF, ciliary neurotrophic factor

Citations for Mouse/Rat CNTF Antibody (5)

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Citations are publications that use Bio-Techne products. Selected citations for Mouse/Rat CNTF Antibody include:

Species: Mouse
Sample Types: Cell Lysate
Applications: Western Blot

P Gómez-Fern et al. (2020), The Rare IL22RA2 Signal Peptide Coding Variant rs28385692 Decreases Secretion of IL-22BP Isoform-1, -2 and -3 and Is Associated with Risk for Multiple Sclerosis Cells, 9(1).
PMID: 31936765
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

GJ Moon et al. (2019), Therapeutic Potential of AAV1-Rheb(S16H) Transduction Against Alzheimer's Disease J Clin Med, 8(12).
PMID: 31766645
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Severi I et al. (2013), Opposite effects of a high-fat diet and calorie restriction on ciliary neurotrophic factor signaling in the mouse hypothalamus. Front Neurosci, 7(0):263.
PMID: 24409114
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC

Leaver SG et al. (2006), AAV-mediated expression of CNTF promotes long-term survival and regeneration of adult rat retinal ganglion cells. Gene Ther., 13(18):1328-41.
PMID: 16708079
Species: Mouse
Sample Types: Tissue Homogenates
Applications: Western Blot

Gard AL et al. (2004), Cardiotrophin-1 in choroid plexus and the cerebrospinal fluid circulatory system. Neuroscience, 127(1):43-52.
PMID: 15219667

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Mouse/Rat CNTF Antibody

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Applications for Mouse/Rat CNTF Antibody

Scientific Data Examples for Mouse/Rat CNTF Antibody

Immunohistochemistry

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Immunohistochemistry

Neutralization

Formulation, Preparation and Storage

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Background: CNTF

Citations for Mouse/Rat CNTF Antibody (5)

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