Recombinant Rat CNTF Protein

Name: Recombinant Rat CNTF Protein
Brand: R&D Systems
SKU: 557-NT
Rating: 5 (1 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 557-NT

Citations (34)

(1)

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Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Accession #:	P20294.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Rat CNTF Protein

Product Specifications for Recombinant Rat CNTF Protein

Source	E. coli-derived rat CNTF protein Ala2-Met200
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala2
Predicted Molecular Mass	22.8 kDa
Activity	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 3‑15 ng/mL. Measured by its ability to support the survival and stimulate neurite outgrowth of dissociated chick embryonic dorsal root ganglia (DRG) neurons. Davies, A.M. (1989) in Neurotrophic Factor Bioassay Using Dissociated Neurons, Nerve Growth Factor. Rush, R.A. (eds): John Willey and Sons, Ltd. 95. The ED₅₀ for this effect is 0.1-0.3 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

557-NT

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

557-NT/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: CNTF

Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy.

The cDNA for CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL‑6, IL-11, LIF, and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes. For a list of CNTF references, see the R&D Systems’ cytokine catalog.

Item	Value
Long Name	Ciliary Neurotrophic Factor
Entrez Gene IDs	1270 (Human); 12803 (Mouse); 25707 (Rat)
Alternate Names	CNTF, HCNTF, ciliary neurotrophic factor

Citations for Recombinant Rat CNTF Protein (34)

Showing 1 - 10 of 34 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Rat CNTF Protein include:

Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

G D'Este et al. (2022), Latrotoxin-Induced Neuromuscular Junction Degeneration Reveals Urocortin 2 as a Critical Contributor to Motor Axon Terminal Regeneration International Journal of Molecular Sciences, 23(3).
PMID: 35163106
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Martin et al. (2022), Oligodendrocyte differentiation alters tRNA modifications and codon optimality-mediated mRNA decay Nature Communications, 13(1):5003.
PMID: 36008413
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

F Bucher et al. (2020), CNTF Prevents Development of Outer Retinal Neovascularization Through Upregulation of CxCl10 Invest. Ophthalmol. Vis. Sci., 61(10):20.
PMID: 32780864
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

KC Allan et al. (2020), Non-canonical Targets of HIF1a Impair Oligodendrocyte Progenitor Cell Function Cell Stem Cell, 0(0).
PMID: 33091368
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

KM Collura et al. (2020), The palmitoyl acyltransferases ZDHHC5 and ZDHHC8 are uniquely present in DRG axons and control retrograde signaling via the Gp130/JAK/STAT3 pathway J. Biol. Chem., 0(0).
PMID: 32958558
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

C Soulard et al. (2020), Spinal Motoneuron TMEM16F Acts at C-boutons to Modulate Motor Resistance and Contributes to ALS Pathogenesis Cell Rep, 30(8):2581-2593.e7.
PMID: 32101737
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

F Bucher et al. (2020), CNTF Prevents Development of Outer Retinal Neovascularization Through Upregulation of CxCl10 Invest. Ophthalmol. Vis. Sci., 61(10):20.
PMID: 32780864
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

C Wu et al. (2019), MAP4K4 Activation Mediates Motor Neuron Degeneration in Amyotrophic Lateral Sclerosis Cell Rep, 26(5):1143-1156.e5.
PMID: 30699345
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

N Funk et al. (2019), The Parkinson's disease-linked Leucine-rich repeat kinase 2 (LRRK2) is required for insulin-stimulated translocation of GLUT4 Sci Rep, 9(1):4515.
PMID: 30872638
Species: Rat
Sample Types: Whole Cells
Applications: Cell Culture

H Lindahl et al. (2019), IL-22 Binding Protein Promotes the Disease Process in Multiple Sclerosis J. Immunol., 0(0).
PMID: 31292217
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

H Munezane et al. (2019), Roles of Collagen XXV and Its Putative Receptors PTPsigma/delta in Intramuscular Motor Innervation and Congenital Cranial Dysinnervation Disorder Cell Rep, 29(13):4362-4376.e6.
PMID: 31875546
Species: Rat
Sample Types: Whole Tissue
Applications: Bioassay

R Rajagopal et al. (2018), Retinal de novo lipogenesis coordinates neurotrophic signaling to maintain vision JCI Insight, 3(1).
PMID: 29321376
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

KL Gibbs et al. (2018), Inhibiting p38 MAPK alpha rescues axonal retrograde transport defects in a mouse model of ALS Cell Death Dis, 9(6):596.
PMID: 29789529
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

J Di Pierdom et al. (2018), Neuroprotective Effects of FGF2 and Minocycline in Two Animal Models of Inherited Retinal Degeneration Invest. Ophthalmol. Vis. Sci., 59(11):4392-4403.
PMID: 30193320
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Schaller et al. (2017), Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons Proc. Natl. Acad. Sci. U.S.A, 114(12):E2486-E2493.
PMID: 28270618
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

SP Jenkinson et al. (2017), Embryonic Stem Cell-Derived Neurons Grown on Multi-Electrode Arrays as a Novel In vitro Bioassay for the Detection of Clostridium botulinum Neurotoxins Front Pharmacol, 8(0):73.
PMID: 28280466
Species: Rat
Sample Types: Whole Tissue
Applications: Bioassay

(2016), A system to study mechanisms of neuromuscular junction development and maintenance Development, 0(0).
PMID: 27226316
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), CNTF Attenuates Vasoproliferative Changes Through Upregulation of SOCS3 in a Mouse-Model of Oxygen-Induced Retinopathy Invest Ophthalmol Vis Sci, 57(10):4017-26.
PMID: 27494343
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Schmieg N et al. (2015), Novel Kidins220/ARMS Splice Isoforms: Potential Specific Regulators of Neuronal and Cardiovascular Development. PLoS ONE, 10(6):e0129944.
PMID: 26083449
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Terenzio M et al. (2014), Bicaudal-D1 regulates the intracellular sorting and signalling of neurotrophin receptors. EMBO J, 33(14):1582-98.
PMID: 24920579
Species: Zebrafish
Sample Types: Whole Tissue
Applications: Bioassay

Elsaeidi et al. (2014), Jak/Stat signaling stimulates zebrafish optic nerve regeneration and overcomes the inhibitory actions of Socs3 and Sfpq. J Neurosci, 34(7):2632-44.
PMID: 24523552
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Severi I et al. (2013), Opposite effects of a high-fat diet and calorie restriction on ciliary neurotrophic factor signaling in the mouse hypothalamus. Front Neurosci, 7(0):263.
PMID: 24409114
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Krencik R et al. (2011), Specification of transplantable astroglial subtypes from human pluripotent stem cells. Nat. Biotechnol., 29(6):528-34.
PMID: 21602806
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Dubovy P et al. (2011), Ciliary neurotrophic factor promotes motor reinnervation of the musculocutaneous nerve in an experimental model of end-to-side neurorrhaphy. BMC Neurosci, 12(0):58.
PMID: 21696588
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Kent TL et al. (2008), Chronic intravitreous infusion of ciliary neurotrophic factor modulates electrical retinal stimulation thresholds in the RCS rat. Invest. Ophthalmol. Vis. Sci., 49(1):372-9.
PMID: 18172115
McGill TJ et al. (2007), Intraocular CNTF reduces vision in normal rats in a dose-dependent manner. Invest. Ophthalmol. Vis. Sci., 48(12):5756-66.
PMID: 18055829
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

Nakanishi M et al. (2007), Microglia-derived interleukin-6 and leukaemia inhibitory factor promote astrocytic differentiation of neural stem/progenitor cells. Eur. J. Neurosci., 25(3):649-58.
PMID: 17328769
Species: Chicken
Sample Types: Whole Cells
Applications: Bioassay

Ko ML et al. (2007), The expression of L-type voltage-gated calcium channels in retinal photoreceptors is under circadian control. J. Neurochem., 103(2):784-92.
PMID: 17683482
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Murata T et al. (2006), Protective effects of neurotrophic factors on tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis of murine adrenal chromaffin cell line tsAM5D. J. Biol. Chem., 281(32):22503-16.
PMID: 16772303
Species: Avian
Sample Types: Whole Cells
Applications: Bioassay

Hapner SJ et al. (2006), NT-3 and CNTF exert dose-dependent, pleiotropic effects on cells in the immature dorsal root ganglion: neuregulin-mediated proliferation of progenitor cells and neuronal differentiation. Dev. Biol., 297(1):182-97.
PMID: 16784738
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Janoschek R et al. (2006), gp130 signaling in proopiomelanocortin neurons mediates the acute anorectic response to centrally applied ciliary neurotrophic factor. Proc. Natl. Acad. Sci. U.S.A., 103(28):10707-12.
PMID: 16818888
Species: Rat
Sample Types: Whole Tissue
Applications: Bioassay

Schmidt-Ott KM et al. (2005), Novel regulators of kidney development from the tips of the ureteric bud. J. Am. Soc. Nephrol., 16(7):1993-2002.
PMID: 15917337
Species: Chicken
Sample Types: In Vivo
Applications: In Vivo

Fischer AJ et al. (2004), Different aspects of gliosis in retinal Muller glia can be induced by CNTF, insulin, and FGF2 in the absence of damage. Mol. Vis., 10(0):973-86.
PMID: 15623987
Species: Rat
Sample Types: In Vivo
Applications: In Vivo

Mizisin AP et al. (2004), Ciliary neurotrophic factor improves nerve conduction and ameliorates regeneration deficits in diabetic rats. Diabetes, 53(7):1807-12.
PMID: 15220205

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Recombinant Rat CNTF Protein

Name: Michelle Chen

Application: CellProlif

Verified Customer | Posted 05/10/2017

Recombinant Rat CNTF Protein

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Product Specifications for Recombinant Rat CNTF Protein

Formulation, Preparation and Storage

Carrier Free

557-NT

Carrier

557-NT/CF

Carrier Free

Reconstitution Calculator

Background: CNTF

Citations for Recombinant Rat CNTF Protein (34)

Customer Reviews for Recombinant Rat CNTF Protein (1)

Recombinant Rat CNTF Protein

Product Documents for Recombinant Rat CNTF Protein

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