Recombinant Human LIF Protein

Name: Recombinant Human LIF Protein
Brand: R&D Systems
SKU: 7734-LF
Rating: 4 (3 reviews)

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 7734-LF

Citations (24)

(3)

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Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Accession #:	P15018
Applications:	Bioactivity

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Product Specifications for Recombinant Human LIF Protein

Source	E. coli-derived human LIF protein Pro24-Phe202
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Pro24
Predicted Molecular Mass	19.6 kDa
SDS-PAGE	19 kDa, reducing conditions
Activity	Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED₅₀ for this effect is 0.02-0.12 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

7734-LF

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

7734-LF/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: LIF

LIF (leukemia inhibitory factor) is a widely expressed, highly and variably glycosylated, 32‑62 kDa, monomeric, pleiotropic cytokine in the IL‑6 family of helical cytokines (1‑4). The first exon encoding the signal sequence is alternately spliced, resulting in LIF-D, LIF-M, and LIF‑T mRNAs that produce secreted, extracellular matrix‑associated, and intracellular forms, respectively (5). LIF-D and LIF-M mRNAs produce identical 180 amino acid (aa) mature sequences (5). Mature human LIF (180 aa) shares 78%, 82%, 91%, 88 and 87% aa sequence identity with mouse, rat, canine, bovine, and porcine LIF, respectively. The LIF receptor is a heterodimer of a type I transmembrane ligand‑binding subunit, LIFR (gp190), and the type I transmembrane signal transducing subunit, gp130, signaling especially through STAT3 and JAK kinases (3, 4, 6). Gp130 and members of the LIFR family also mediate the biological effects of Oncostatin M, Cardiotrophin‑1, Galectin‑10, CNTF, IL‑6,
IL‑11, and IL‑27 (3, 6). A soluble LIFR has been reported in the mouse (7). Depending on the cells and their context, LIF either opposes or favors differentiation (3, 8). LIF produced by the uterine endometrium supports successful implantation of the embryo, promotes proliferation and maintenance of pluripotency in embryonic stem cells, and favors proliferation of progenitor cell types such as hematopoietic stem cells (3, 6, 8). However, excess LIF blocks differentiation of embryoid bodies, indicating the importance of LIF regulation (3, 6). LIF is produced by CD4⁺ T cells in response to activation, and is required by the thymic epithelium to support T cell maturation (3, 4). LIF expression is up‑regulated by neuronal injury, and promotes motor neuron survival and oligodendrocyte myelination (3, 4, 9). LIF is produced by the adrenal cortex and likely enhances its production of cortisol and aldosterone (10). LIF can function as an autocrine growth factor in some pancreatic cancers, but induces differentiation in the myeloid leukemic cell line M1 (2, 11). Tumor LIF can also induce formation of immunosuppressive tumor‑associated macrophages (12). LIF promotes endometrial remodeling and differentiation of adipocytes and cardiac smooth muscle cells (3, 4). It promotes regulatory T cell and inhibits Th17 cell differentiation, thus promoting tolerance, down‑regulating inflammation, and contributing to immune tolerance during pregnancy and in the nervous system (3, 4, 6, 8).

Item	Value
References	Gough, N.M. et al. (1988) Proc. Natl. Acad. Sci. USA 85:2623. Moreau, J.F. et al. (1988) Nature 336:690. Trouillas, M. et al. (2009) Eur. Cytokine Netw. 20:51. Metcalfe, S.M. (2011) Genes Immun. 12:157. Voyle, R.B. et al. (1999) Exp. Cell Res. 249:199. Cheng, J.G. et al. (2001) Proc. Natl. Acad. Sci. USA 98:8680. Tomida, M. et al. (1993) FEBS lett. 334:193. Paiva, P. et al. (2009) Cytokine Growth Factor Rev. 20:319. Slaets, H. et al. (2010) Trends Mol. Med. 16:493. Bamberger, A.M. et al. (2000) Mol. Cell. Endocrinol. 162:145. Kamohara, H. et al. (2007) Int. J. Oncol. 30:977. Duluc, D. et al. (2007) Blood 110:4319.
Long Name	Leukemia Inhibitory Factor
Entrez Gene IDs	3976 (Human); 16878 (Mouse); 403449 (Canine)
Alternate Names	CDF, D Factor, DIA, Differentiation-stimulating factor, Emfilermin, HILDA, HILDAcholinergic differentiation factor, LIF, MLPLI, Melanoma-derived LPL inhibitor, differentiation inhibitory activity, differentiation stimulating factor, leukemia inhibitory factor, leukemia inhibitory factor (cholinergic differentiation factor)

Citations for Recombinant Human LIF Protein (24)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Human LIF Protein include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SW Wang et al. (2022), CoSpar identifies early cell fate biases from single-cell transcriptomic and lineage information Nature Biotechnology, 0(0).
PMID: 35190690
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

M Lindkvist et al. (2022), Pleiotropic, Unique and Shared Responses Elicited by IL-6 Family Cytokines in Human Vascular Endothelial Cells International Journal of Molecular Sciences, 23(3).
PMID: 35163371
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

W Yuan et al. (2021), The histone demethylase KDM2B regulates human primordial germ cell-like cells specification International journal of biological sciences, 17(2):527-538.
PMID: 33613110
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

ME Clements et al. (2021), HDAC inhibitors induce LIFR expression and promote a dormancy phenotype in breast cancer Oncogene, 0(0).
PMID: 34247191
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Y Fan et al. (2021), Generation of human blastocyst-like structures from pluripotent stem cells Cell Discovery, 7(1):81.
PMID: 34489415
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

YC Wen et al. (2021), PCK1 regulates neuroendocrine differentiation in a positive feedback loop of LIF/ZBTB46 signalling in castration-resistant prostate cancer British Journal of Cancer, 0(0).
PMID: 34815524
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Wang et al. (2021), Leukemia inhibitory factor is involved in the pathogenesis of NSCLC through activation of the STAT3 signaling pathway Oncology Letters, 22(3):663.
PMID: 34386085
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

F Fang et al. (2020), Analysis of multi-lineage gene expression dynamics during primordial germ cell induction from human induced pluripotent stem cells Stem Cell Res Ther, 11(1):100.
PMID: 32127035
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

SR Lin et al. (2020), EGFR-upregulated LIFR promotes SUCLG2-dependent castration resistance and neuroendocrine differentiation of prostate cancer Oncogene, 0(0).
PMID: 32963351
Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

Z Li et al. (2019), Supplementation of vitamin C promotes early germ cell specification from human embryonic stem cells Stem Cell Res Ther, 10(1):324.
PMID: 31730021
Species: Human
Sample Types: Whole Cells
Applications: Surface Plasmon Resonance

S Viswanadha et al. (2019), EC359-A first-in-class small molecule inhibitor for targeting oncogenic LIFR signaling in triple negative breast cancer Mol. Cancer Ther., 0(0).
PMID: 31142661
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

L Alabdi et al. (2018), The transcription factor Vezf1 represses the expression of the antiangiogenic factor d2 in endothelial cells J. Biol. Chem., 0(0).
PMID: 29794136
Species: Bovine
Sample Types: Whole Cells
Applications: Bioassay

A Tscherner et al. (2018), STAT3 signaling stimulates miR-21 expression in bovine cumulus cells during in vitro oocyte maturation Sci Rep, 8(1):11527.
PMID: 30068990
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Li et al. (2018), LIFR increases the release of sEng via the up-regulation of MMP14 expression in PE Reproduction, 0(0).
PMID: 29363569
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

S Jiao et al. (2017), miR-124 promotes proliferation and differentiation of neuronal stem cells through inactivating Notch pathway Cell Biosci, 7(0):68.
PMID: 29238518
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

H Zhang et al. (2017), Combining Injectable Plasma Scaffold with Mesenchymal Stem/Stromal Cells for Repairing Infarct Cavity after Ischemic Stroke Aging Dis, 8(2):203-214.
PMID: 28400986
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Enrique Armijo (2016), Increased susceptibility to a? toxicity in neuronal cultures derived from familial Azheimer's disease (PSEN1-A246E) induced pluripotent stem cells Neurosci. Lett, 0(0).
PMID: 28034781
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016), Induction of LIFR confers a dormancy phenotype in breast cancer cells disseminated to the bone marrow Nat Cell Biol, 18(10):1078-1089.
PMID: 27642788
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Geng et al. (2015), Targeted Knockdown of RNA-Binding Protein TIAR for Promoting Self-Renewal and Attenuating Differentiation of Mouse Embryonic Stem Cells. Stem Cells Int, 2015(0):657325.
PMID: 25918534
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Hong S et al. (2015), A Novel Small-Molecule Inhibitor Targeting the IL-6 Receptor beta Subunit, Glycoprotein 130. J Immunol, 195(1):237-45.
PMID: 26026064
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kwofie K et al. (2015), Regulation of IL-17A responses in human airway smooth muscle cells by Oncostatin M. Respir Res, 16(0):14.
PMID: 25849622
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Lapeire L et al. (2014), Cancer-associated adipose tissue promotes breast cancer progression by paracrine oncostatin M and Jak/STAT3 signaling. Cancer Res, 74(23):6806-19.
PMID: 25252914
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Wagener E et al. (2014), The amino acid exchange R28E in ciliary neurotrophic factor (CNTF) abrogates interleukin-6 receptor-dependent but retains CNTF receptor-dependent signaling via glycoprotein 130 (gp130)/leukemia inhibitory factor receptor (LIFR). J Biol Chem, 289(26):18442-50.
PMID: 24802752
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Kondo T et al. (2014), Focal transplantation of human iPSC-derived glial-rich neural progenitors improves lifespan of ALS mice. Stem Cell Reports, 3(2):242-9.
PMID: 25254338

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Customer Reviews for Recombinant Human LIF Protein (3)

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Recombinant Human LIF Protein

Name: Anonymous

Application: Apoptosis assay

Verified Customer | Posted 04/17/2020
Recombinant Human LIF Protein

Name: Anonymous

Application: Cell migration/motility

Verified Customer | Posted 04/09/2017

Growth of MA-11 spheroids in serum-free condition in the absence (A) or presence (B) of Recombinant Human LIF (#7734-LF) after 72 hours. Medium: RPMI + 2% B-27 supplement + 1% L-glutamine + 1% Pen/Strep.
Recombinant Human LIF Protein

Name: Anonymous

Application: Stem/Immune cell maintenance or differentiation

Verified Customer | Posted 08/03/2016

Recombinant Human LIF Protein

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Complete Your Research

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Product Specifications for Recombinant Human LIF Protein

Formulation, Preparation and Storage

Carrier Free

7734-LF

Carrier

7734-LF/CF

Carrier Free

Reconstitution Calculator

Background: LIF

Citations for Recombinant Human LIF Protein (24)

Customer Reviews for Recombinant Human LIF Protein (3)

Customer Images

Recombinant Human LIF Protein

Recombinant Human LIF Protein

Recombinant Human LIF Protein

Product Documents for Recombinant Human LIF Protein

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