DL-AP5 Sodium salt

Name: DL-AP5 Sodium salt
Brand: Tocris Bioscience
SKU: 3693
Rating: 5 (2 reviews)

Tocris Bioscience a Bio-Techne Brand

Catalog # 3693

Citations (8)

(2)

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Key Product Details

Description:	Potent and selective NMDA antagonist; sodium salt of DL-AP5
Chemical Name:	DL-2-Amino-5-phosphonopentanoic acid sodium salt
Purity:	≥98% (HPLC)
Molecular Weight:	219.11

View Full Technical Details for DL-AP5 Sodium salt

Biological Activity for DL-AP5 Sodium salt

DL-AP5 Sodium salt is the sodium salt of DL-AP5 (Cat. No. 0105) and a racemic mixture of the D- and L-isomers of AP5, a selective NMDA receptor antagonist that competes with glutamate binding and is commonly used to inhibit NMDA-dependent synaptic plasticity. D-AP5 (Cat. No. 0106) is the more active isomer and displays approximately 52-fold higher potency than the L-isomer, L-AP5 (Cat. No. 0107). In vitro D-AP5 reduces NMDA-induced depolarization of cortical neurons, with no effect on the response to L-Quisqualic acid (Cat. No. 0188) or Kainic acid (Cat. No. 0222). Following spinal injection of D-AP5, NMDA-response is rapidly reduced, with no effect seen on spontaneously active neurons.

DL mixture, D-isomer and L-isomer also available.

Technical Data for DL-AP5 Sodium salt

M. Wt	219.11
Formula	C₅H₁₁NNaO₅P
Storage	Desiccate at RT
Purity	≥98% (HPLC)
CAS Number	1303993-72-7
PubChem ID	52974251
InChi Key	KWRCYAPNGUCHOE-UHFFFAOYSA-M
Smiles	[Na+].NC(CCCP(O)([O-])=O)C(O)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for DL-AP5 Sodium salt

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	water	21.91	100

Preparing Stock Solutions for DL-AP5 Sodium salt

The following data is based on the product molecular weight 219.11.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Select a batch to recalculate based on the batch molecular weight:

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	4.56 mL	22.82 mL	45.64 mL
5 mM	0.91 mL	4.56 mL	9.13 mL
10 mM	0.46 mL	2.28 mL	4.56 mL
50 mM	0.09 mL	0.46 mL	0.91 mL

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References for DL-AP5 Sodium salt

References are publications that support the biological activity of the product.

Evans The effect of a series of ω-phosphonic-α-carboxylic amino acids on electrically evoked and amino acid induced responses in isolated spinal cord preparations. Br.J.Pharmacol. 1982 PMID: 7042024
Davies and Watkins Actions of D and L forms of 2-amino-5-phosphonovalerate and 2-amino-4-phosphonobutyrate in the cat spinal cord. Brain Res. 1982 PMID: 6145492

Citations for DL-AP5 Sodium salt (8)

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Citations are publications that use Bio-Techne products. Selected citations for DL-AP5 Sodium salt include:

Pati et al. (2020) Chronic postnatal chemogenetic activation of forebrain excitatory neurons evokes persistent changes in mood behavior ELife. 9
PMID: 32955432
Zhao et al. (2018) Soluble Aβ Oligomers Impair Dipolar Heterodendritic Plasticity by Activation of mGluR in the Hippocampal CA1 Region. iScience. 6:138
PMID: 30240608
Athilingam et al. (2017) Serotonin enhances excitability and gamma frequency temporal integration in mouse prefrontal fast-spiking interneurons. Elife. 6
PMID: 29206101
Maschi and Klyachko (2017) Spatiotemporal Regulation of Synaptic Vesicle Fusion Sites in Central Synapses. Neuron. 94:65
PMID: 28343869
Thomsen et al. (2016) Fixed single-cell transcriptomic characterization of human radial glial diversity. Sci Rep. 13:87
PMID: 26524239
Pan et al. (2016) Postnatal development of the electrophysiological properties of somatostatin interneurons in the anterior cingulate cortex of mice. PLoS One. 6:28137
PMID: 27319800
Cazares et al. (2016) Dynamic Partitioning of Synaptic Vesicle Pools by the SNARE-Binding Protein Tomosyn. J Neurosci. 36:11208
PMID: 27807164
Falgairolle and O'Donovan (2015) Pharmacological Investigation of Fluoro-Gold Entry into Spinal Neurons. Purinergic Signal. 10:e0131430
PMID: 26102354

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Customer Reviews for DL-AP5 Sodium salt (2)

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Works as expected.

Name: Anonymous

Species: Mouse

Assay Type: Ex Vivo

Cell Line/Tissue: Paraventricular nucleus of hypothalamus

DL-AP5 (40 micromolar) blocked spontaneous excitatory postsynaptic currents in an ex-vivo slice preparation.

PMID: 32133707
NMDA glutamate site antagonist

Name: Syed Ali

Species: Mouse

Assay Type: Ex Vivo

This small molecule was used as NMDA glutamate site antagonist in ex-vivo patch clamp electrophysiology to investigate neuronal excitability.

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Product Documents for DL-AP5 Sodium salt

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.