Compound E

Name: Compound E
Brand: Tocris Bioscience
SKU: 6476

Tocris Bioscience a Bio-Techne Brand

Catalog # 6476

Citations (2)

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Key Product Details

Description:	γ-secretase inhibitor; induces neuronal differentiation
Alternative Names:	γ-Secretase Inhibitor XXI
Chemical Name:	N-[(1S)-2-[[(3S)-2,3-Dihydro-1-methyl-2-oxo-5-phenyl-1H-1,4-benzodiazepin-3-yl]amino]-1-methyl-2-oxoethyl]-3,5-difluorobenzeneacetamide
Purity:	≥98% (HPLC)
Molecular Weight:	490.5

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Biological Activity for Compound E

Compound E is a γ-secretase and notch patchway inhibitor. Compound E enhances growth inhibition, differentiation and migration of neuroblastoma cells when used in a combination with DAPT (Cat. No. 2634) and 13-cis RA. In combination with hLIF, CHIR 99021 (Cat. No. 4423) and SB 431542 (Cat. No. 1614), Compound E accelerates the induction of a homogeneous, self-renewing primitive neuroepithelium population from hESCs. Compound E is active in vitro and in vivo.

For more information about how Compound E may be used, see our protocol: Generation of β cells from hPSCs

Technical Data for Compound E

M. Wt	490.5
Formula	C₂₇H₂₄F₂N₄O₃
Storage	Store at -20°C
Purity	≥98% (HPLC)
CAS Number	209986-17-4
PubChem ID	11306390
InChi Key	JNGZXGGOCLZBFB-IVCQMTBJSA-N
Smiles	C[C@@H](C(N[C@H]1N=C(C2=CC=CC=C2N(C1=O)C)C3=CC=CC=C3)=O)NC(CC4=CC(F)=CC(F)=C4)=O

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.

Tocris products are intended for laboratory research use only, unless stated otherwise.

Solubility for Compound E

	Solvent	Max Conc. mg/mL	Max Conc. mM
Solubility
Solubility	DMSO	49.05	100

Preparing Stock Solutions for Compound E

The following data is based on the product molecular weight 490.5.

Batch specific molecular weights may vary from batch to batch due to the degree of hydration, which will affect the solvent volumes required to prepare stock solutions.

Concentration / Solvent Volume / Mass	1 mg	5 mg	10 mg
1 mM	2.04 mL	10.19 mL	20.39 mL
5 mM	0.41 mL	2.04 mL	4.08 mL
10 mM	0.20 mL	1.02 mL	2.04 mL
50 mM	0.04 mL	0.20 mL	0.41 mL

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References for Compound E

References are publications that support the biological activity of the product.

Li Rapid induction and long-term self-renewal of primitive neural precursors from human embryonic stem cells by small molecule inhibitors. Proc.Natl.Acad.Sci.USA 2011 PMID: 21525408
Ferrari-Toninelli Targeting Notch pathway induces growth inhibition and differentiation of neuroblastoma cells. Neuro.Oncol. 2010 PMID: 20716592
Beher Pharmacological knock-down of the presenilin 1 heterodimer by a novel gamma -secretase inhibitor: implications for presenilin biology. J.Biol.Chem. 2001 PMID: 11574530

Citations for Compound E (2)

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Citations are publications that use Bio-Techne products. Selected citations for Compound E include:

Zhan et al. (2022) Pancreatic islet cryopreservation by vitrification achieves high viability, function, recovery and clinical scalability for transplantation. Nat.Med.. 28:798
PMID: 35288694
Sheng et al. (2018) A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation. Nat Commun. 9:4047
PMID: 30279449

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Product Documents for Compound E

COA

Certificate of Analysis

To download a Certificate of Analysis, please select a batch below.